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Method development and acid degradation study of doxofylline by rp-hplc and lc-ms/ms

Gupta, Akhilesh (2016)
Publisher: Figshare
A simple, rapid and accurate RP-HPLC method was developed for the determination of doxofylline and aciddegradation product. The method showed a linear response for concentrations in the range of 1-200 g/ml usingacetonitrile: formic Acid (90: 10); pH-3.0 as the mobile phase with detection at 274 nm and a flow rate of 1 ml/minand retention time 2.9 min. The method was statistically validated for accuracy, precision, linearity, ruggedness,robustness, forced degradation, solution stability and se...

Summary of accessions sampled forWaxy gene analysis.

LR: Landrace; IL: Inbred line; CAAS: Chinese Academy of Agricultural Sciences; SAGC: Shanghai Agrobiological Gene Center; SAAS: Shanghai Academy of Agricultural Sciences.

Lgr5 Methylation in Cancer Stem Cell Differentiation and Prognosis-Prediction in Colorectal Cancer

ObjectiveLeucine-rich-repeat-containing G-protein-coupled receptor 5 (lgr5) is a candidate marker for colorectal cancer stem cells (CSC). In the current study, we investigated the methylation status within thelgr5 promoter and evaluated its relationship with CSC differentiation, prognosis for colorectal cancer, and its clinicopathological features.MethodsThe methylation status within Lgr5 promoter was detected with a methylation-specific PCR in six colorectal cancer cell lines as well as 169 ...

Factors associated with a positive PCR among 565 snake bite victims.

Unadjusted Risk Ratio (RR) and their 95% Confidence Interval (95% CI) were calculated with respect to the baseline category, i.e., absence of the risk factor (RR = 1).

Demographic data and results.

Demographic data, [Na+] and H2O content in the muscle, the skin and the lower leg, Na+/NT-proBNP blood levels and body weight in patients with acute heart failure before and after diuretic (furosemide) therapy and on the control group are presented (mean ± SD). P-values (two-tailed student’s t-test) and confidence intervals (95% confidence level) are shown. BT = before therapy, AT = after therapy, C = controls (healthy subjects). AU = arbitrary unit. NA = not applicable.Demographic data and r...

Model spore trajectories for seven basidiomycetes species based on measurements of spore size and launch speed and using Stokes model of viscous drag.

To aid visualization, spores were launched horizontally from arbitrary heights. Positions of spores at 50 µs intervals indicated by dots. The variation in horizontal range predicted from the measured variation in launch speeds (±standard error; Table 1) for each species is represented by the shaded region around each trajectory. Species initials: G.j.-v., Gymnosporangium juniperi-virginianae; T.c., Tilletia caries; S.s., Sporobolomyces salmonicolor; A.a., Auricularia auricula; P.s., Polyporus...

Mitochondrial Haplogroup H1 in North Africa: An Early Holocene Arrival from Iberia

The Tuareg of the Fezzan region (Libya) are characterized by an extremely high frequency (61%) of haplogroup H1, a mitochondrial DNA (mtDNA) haplogroup that is common in all Western European populations. To define how and when H1 spread from Europe to North Africa up to the Central Sahara, in Fezzan, we investigated the complete mitochondrial genomes of eleven Libyan Tuareg belonging to H1. Coalescence time estimates suggest an arrival of the European H1 mtDNAs at about 8,000–9,000 years ago,...