Wu, Na; Wei, Huanhuan; Zhang, Lizhi (2012)
We demonstrated that mesoporous titania beads of uniform
450 μm) and high surface area could be synthesized via an alginate
biopolymer template method. These mesoporous titania beads could efficiently
remove Cr(VI), Cd(II), Cr(III), Cu(II), and Co(II) ions from simulated
wastewater with a facile subsequent solid–liquid separation
because of their large sizes. We chose Cr(VI) removal as the case
study and found that each gram of these titania beads could ...
Shlyakhtenko, Luda S.; Lushnikov, Alexander Y.; Miyagi, Atsushi; Li, Ming; Harris, Reuben S.; Lyubchenko, Yuri L. (2012)
The DNA cytosine deaminase APOBEC3G (A3G) is capable
of blocking retrovirus replication by editing viral cDNA and impairing
reverse transcription. However, the biophysical details of this host–pathogen
interaction are unclear. We applied atomic force microscopy (AFM)
and hybrid DNA substrates to investigate properties of A3G bound to
single-stranded DNA (ssDNA). Hybrid DNA substrates included ssDNA
with 5′ or 3′ ends attached to DNA duplexes (tail-DNA)
and gap-DNA substra...
Kang, Jeon Woong; Nguyen, Freddy
T.; Lue, Niyom; Dasari, Ramachandra R.; Heller, Daniel A. (2016)
Carbon nanotube uptake was measured via high-speed confocal
Raman imaging in live cells. Spatial and temporal tracking of two
cell-intrinsic and nine nanotube-derived Raman bands was conducted
simultaneously in RAW 264.7 macrophages. Movies resolved single (n, m) species, defects, and aggregation
states of nanotubes transiently as well as the cell position, denoted
by lipid and protein signals. This work portends the real-time molecular
imaging of live cells and tissues usin...
Cheng, Shih-Hsun; Li, Feng-Chieh; Souris, Jeffrey S.; Yang, Chung-Shi; Tseng, Fan-Gang; Lee, Hsuan-Shu; Chen, Chin-Tu; Dong, Chen-Yuan; Lo, Leu-Wei (2016)
Nanoparticles that do not undergo renal excretion or in vivo degradation into biocompatible debris often accumulate in the reticuloendothelial system, also know as the mononuclear phagocyte system, with undesired consequences that limit their clinical utility. In this work, we report the first application of intravital multiphoton fluorescence microscopy to dynamically track the hepatic metabolism of nanoparticles with subcellular resolution in real time. Using fluorescently labeled mesoporou...
W. J. de Folter, Julius; Hutter, Eline M.; I. R. Castillo, Sonja; Klop, Kira E.; Philipse, Albert P.; Kegel, Willem K. (2014)
We have investigated the effect of
particle shape in Pickering
emulsions by employing, for the first time, cubic and peanut-shaped
particles. The interfacial packing and orientation of anisotropic
microparticles are revealed at the single-particle level by direct
microscopy observations. The uniform anisotropic hematite microparticles
adsorb irreversibly at the oil–water interface in monolayers
and form solid-stabilized o/w emulsions via the process of limited
L.; Harvey, Katherine L.; Stewart, Rebecca J.; Azevedo, Mauro F.; Crabb, Brendan S.; Jennings, Ian G.; Sanders, Paul R.; Manallack, David T.; Thompson, Philip E.; Tonkin, Christopher J.; Gilson, Paul R. (2015)
parasites, including Plasmodium falciparum and Toxoplasma gondii, the causative agents of
severe malaria and toxoplasmosis, respectively, undergo several critical
developmental transitions during their lifecycle. Most important for
human pathogenesis is the asexual cycle, in which parasites undergo
rounds of host cell invasion, replication, and egress (exit), destroying
host cell tissue in the process. Previous work has identified important
roles for Protein ...
Milionis, Athanasios; Fragouli, Despina; Martiradonna, Luigi; Anyfantis, George C.; Cozzoli, P. Davide; Bayer, Ilker
S.; Athanassiou, Athanassia (2014)
Water wetting and
adhesion control on polymeric patterns are achieved
by tuning the configuration of their surface’s structural characteristics
from single to dual and triple length-scale. In particular, surfaces
with combined micro-, submicrometer-,and nanoroughness are developed,
using photolithographically structured SU-8 micro-pillars as substrates
for the consecutive spray deposition of polytetrafluoroethylene (PTFE)
submicrometer particles and hydrophobically capped...
Noh, Hyeran; Phillips, Scott T. (2010)
This article describes an integrated approach to tracking the end point of a time-based assay that is conducted on an analytical device made out of paper. The timing mechanism is built directly into a paper-based analytical device and does not require starting, stopping, reset buttons, batteries, or maintenance; the timer simply starts once the sample is added to the device. These “fluidic timers” are composed of paraffin wax and a signaling feature (e.g., a dye). The timing function is made ...
A.; Yang, Hengwen; Saludes, Jonel P.; Fiorini, Zeno; Beninson, Lida; Chapman, Edwin
R.; Fleshner, Monika; Xue, Ding; Yin, Hang (2013)
Membrane curvature and lipid composition regulates important
processes within a cell. Currently, several proteins have been reported
to sense and/or induce membrane curvatures, e.g.,
Synaptotagmin-1 and Amphiphysin. However, the large protein scaffold
of these curvature sensors limits their applications in complex biological
systems. Our interest focuses on identifying and designing peptides
that can sense membrane curvature based on established elements observ...
Lousa, Diana; Baptista, António M.; Soares, Cláudio M. (2016)
One of the drawbacks of nonaqueous enzymology is the
enzymes tend to be less stable in organic solvents than in water.
There are, however, some enzymes that display very high stabilities
in nonaqueous media. In order to take full advantage of the use of
nonaqueous solvents in enzyme catalysis, it is essential to elucidate
the molecular basis of enzyme stability in these media. Toward this
end, we performed μs-long molecular dynamics simulations using