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7147 research data, page 1 of 715

Mitigation Measures for Pandemic Influenza in Italy: An Individual Based Model Considering Different Scenarios

BackgroundIndividual-based models can provide the most reliable estimates of the spread of infectious diseases. In the present study, we evaluated the diffusion of pandemic influenza in Italy and the impact of various control measures, coupling a global SEIR model for importation of cases with an individual based model (IBM) describing the Italian epidemic.Methodology/Principal FindingsWe co-located the Italian population (57 million inhabitants) to households, schools and workplaces and we a...

Genomic Instability Is Associated with Natural Life Span Variation in Saccharomyces cerevisiae

Qin, Hong; Lu, Meng; S. Goldfarb, David (2008)
Publisher: Figshare
Increasing genomic instability is associated with aging in eukaryotes, but the connection between genomic instability and natural variation in life span is unknown. We have quantified chronological life span and loss-of-heterozygosity (LOH) in 11 natural isolates of Saccharomyces cerevisiae. We show that genomic instability increases and mitotic asymmetry breaks down during chronological aging. The age-dependent increase of genomic instability generally lags behind the drop of viability and t...

The Effect of 24S-Hydroxycholesterol on Cholesterol Homeostasis in Neurons: Quantitative Changes to the Cortical Neuron Proteome

In humans, the brain represents only about 2% of the body’s mass but contains about one-quarter of the body’s free cholesterol. Cholesterol is synthesized de novo in brain and removed by metabolism to oxysterols. 24S-Hydoxycholesterol represents the major metabolic product of cholesterol in brain, being formed via the cytochrome P450 (CYP) enzyme CYP46A1. CYP46A1 is expressed exclusively in brain, normally by neurons. In this study, we investigated the effect of 24S-hydroxycholesterol on the ...

Cross-Species Functionality of Pararetroviral Elements Driving Ribosome Shunting

BackgroundCauliflower mosaic virus (CaMV) and Rice tungro bacilliform virus (RTBV) belong to distinct genera of pararetroviruses infecting dicot and monocot plants, respectively. In both viruses, polycistronic translation of pregenomic (pg) RNA is initiated by shunting ribosomes that bypass a large region of the pgRNA leader with several short (s)ORFs and a stable stem-loop structure. The shunt requires translation of a 5′-proximal sORF terminating near the stem. In CaMV, mutations knocking o...

SDF-1α production in Tac1 knockdown T47D.

T47D cells were stably knockdown for Tac1. Controls were transfected with mutant siRNA. At 80% confluence, culture media were replaced with fresh media and after aliquots of media were collected and then quantitated for SDF-1α levels by ELISA. The results are presented as the mean±SD of four different experiments.*p

Global Regulation of Nucleotide Biosynthetic Genes by c-Myc

BackgroundThe c-Myc transcription factor is a master regulator and integrates cell proliferation, cell growth and metabolism through activating thousands of target genes. Our identification of direct c-Myc target genes by chromatin immunoprecipitation (ChIP) coupled with pair-end ditag sequencing analysis (ChIP-PET) revealed that nucleotide metabolic genes are enriched among c-Myc targets, but the role of Myc in regulating nucleotide metabolic genes has not been comprehensively delineated.Met...

Cell cycle parameters of wt and XL151 strains.

Times are in minutes. Data are the mean of at least 3 independent experiments.(1)data measured.(2)data calculated using the Helmstetter's model (origin per cell = 2(C+D)/t; Helmstetter, 1996). Note that this model originally designed for E. coli B/r has been validated for K12 strains grown in these conditions [56]. Details of origin per cell calculation are in TableS1.(3)Data from flow cytometry experiments.NA: not applicable.

MDT-15 mutation compromises induction of select detoxification genes by fluoranthene (FLA).

Values represent fold-inductions of indicated detoxification genes relative to DMSO-treated N2 worms. Each value is an average±SEM calculated from three independent worm growths and RNA isolations from L4 stage N2 or mdt-15(tm2182) animals, as determined by qPCR; mRNA levels were normalized to act-1. Columns on the right indicate the fold-induction by toxins vs. DMSO within the same genetic background. mdt-15 mutation compromises expression of select detoxification genes; genes whose inductio...