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39 documents, page 4 of 4

Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3

Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation. Plasmid loss during bacterial cell divisions and structural changes in the plasmid DNA can dramatically reduce the yield of the desired recombinant plasmid DNA. While generating an HIV-based gene vector containing a bicistronic expression cassette 5?-Olig2cDNA-IRES-dsRed2-3?, we encountered ...

Electromagnetic model and calculations of the surface-enhanced Raman-shifted emission from Langmuir-Blodgett films on metal nanostructures

We present a theoretical study of the electromagnetic contribution to surface-enhanced Raman scattering (SERS) from a Langmuir-Blodgett film close to a metal surface. This macroscopic dipolar model fully accounts for the Raman-shifted emission so that meaningful SERS (electromagnetic) enhancement factors that do not depend only on the local electromagnetic field enhancement at the pump frequency are defined. For a plane metal surface, analytical SERS enhancement factors that are consistent fo...

Long-range surface polaritons in ultra-thin films of silicon

08.10.14 KB. Ok to add published version to spiral, OSA policy

Innovating standards through informal consortia: The case of wireless telecommunications

We empirically examine the effects of industry consortia on the coordination of innovation strategies of the members. Our analyses utilize membership data from 32 consortia in wireless telecommunication technology subfields from 2000 to 2005 and prior art citations in standards-essential patents. We find that connections among firms in informal technically-oriented consortia significantly increase the likelihood that firms cite each other's patents in subsequent patents essential for the UMTS...

Bus Modelling in Zoned Disks RAID Storage Systems

09.07.14 KB. Ok to add accepted version to spiral.

Challenges and Frugal Remedies for Lowering Facility Based Neonatal Mortality and Morbidity: A Comparative Study

05.09.14 KB. OK to add published version to spiral, OA paper Millennium development goal target on infant mortality (MDG4) by 2015 would not be realised in some low-resource countries. This was in part due to unsustainable high-tech ideas that have been poorly executed. Prudent but high impact techniques could have been synthesised in these countries. A collaborative outreach was initiated to devise frugal measures that could reduce neonatal deaths in Nigeria. Prevailing issues of concern ...

Skin tribology: Science friction?

The application of tribological knowledge is not just restricted to optimizing mechanical and chemical engineering problems. In fact, effective solutions to friction and wear related questions can be found in our everyday life. An important part is related to skin tribology, as the human skin is frequently one of the interacting surfaces in relative motion. People seem to solve these problems related to skin friction based upon a trial-and-error strategy and based upon on our sense for touch....

Modelling land cover change in the Brazilian Amazon: temporal changes in drivers and calibration issues

Land cover change (LCC) models are used in many studies of human impacts on the environment, but knowing how well these models predict observed changes in the landscape is a challenge. We used nearly three decades of LCC maps to run several LCC simulations to: (1) determine which parameters associated with drivers of LCC (e.g. roads) get selected for which transition (forest to deforested, regeneration to deforested or deforested to regeneration); (2) investigate how the parameter values vary...