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54070 documents, page 2 of 5407

Cleavage of Bacteriophage f1 DNA by the Restriction Enzyme of Escherichia coli B

We studied the cleavage of the replicative-form DNA (RF I) of bacteriophage f1 and its SB mutants by purified restriction endonuclease of E. coli B. The results indicate that: (i) Circular replicative forms are broken once to yield full-length linear molecules (RF III). Such linear molecules are less susceptible than RF I to endonuclease R-B. (ii) The genetic sites (SB sites) that confer on the DNA susceptibility to B-restriction are not the actual sites of cleavage. The number of possible cl...

Preliminary observations on the use of the latex agglutination test for detecting circulating trypanosomal antibodies in game animals in the Lambwe Valley

The first use of the latex agglutination test (LAT) for determining the frequency of circulating trypanosomal antibodies in a sample of game animals collected in the Lambwe Valley, Kenya, is described. The immunological examination of animals belonging to 9 species revealed an incidence of circulating antibody of 25.5%, which was only slightly higher than, and therefore in close agreement with, the results of a concurrent parasitological survey. It is concluded that the LAT is very suitable f...

Theory of optimum shapes in free-surface flows. Part 2. Minimum drag profiles in infinite cavity flow

The problem considered here is to determine the shape of a symmetric two-dimensional plate so that the drag of this plate in infinite cavity flow is a minimum. With the flow assumed steady and irrotational, and the effects due to gravity ignored, the drag of the plate is minimized under the constraints that the frontal width and wetted arc-length of the plate are fixed. The extremization process yields, by analogy with the classical Euler differential equation, a pair of coupled nonlinear sin...