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56848 documents, page 4 of 5685

Preparation and some properties of a suspension of fragmented tubules from rat kidney

A method is described whereby short fragments of rat kidney tubule were obtained when kidney slices were gently dispersed by exposure to collagenase and hyaluronidase. When suspended in buffered saline the fragmented tubules respired actively over a period of several hours, the rate of oxygen consumption being proportional to the amount of cell protein. Oxygen uptake was stimulated by the addition of glucose, lactate, butyrate, α-oxoglutarate and other substrates and was decreased by the omis...

QUANTITATIVE FEATURES OF A SANDWICH RADIOIMMUNOLABELING TECHNIQUE FOR LYMPHOCYTE SURFACE RECEPTORS

The present study was designed to devise and characterize an indirect or sandwich radioimmunolabeling technique for the study of lymphocyte surface receptors of immunoglobulin nature. Mouse lymphocytes from various sources were treated by the method of Shortman et al. to remove debris and damaged cells. This was an important preliminary step, as without it, little meaning could be attached to bulk scintillation counting of labeled cell suspensions, in view of the marked tendency of dead or da...

Autoradiography as a tool in primary production research

Autoradiography of C-14 labelled phytoplankton collected onto a millipore filter allows the determination of the number of photosynthetically active cells in a water sample and thus gives useful information on the standing crop in primary production studies.

Bubble nucleation from heated glass cavities.

http://archive.org/details/bubblenucleation00scha NA NA