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Ionic Permeability of the Inhibitory Postsynaptic Membrane of Lobster Muscle Fibers

Reversal potentials (E IPSP) of the inhibitory postsynaptic potential and the membrane resting potentials (EM ) of lobster muscle fibers were determined with intracellular recording under a variety of ionic conditions. E IPSP is solely dependent on the electromotive force of anionic batteries; i.e., on the electrochemical gradient for a "mobile" fraction of intracellular Cl (Cl i ) which is considerably smaller than the total intracellular Cl. The active inhibitory membrane is more permeable ...

Control of Isoleucine, Valine, and Leucine Biosynthesis VI. Effect of 5′,5′,5′-Trifluoroleucine on Repression in Salmonella typhimurium

The leucine analogue 5′,5′,5′,-trifluoroleucine (fluoroleucine) replaced leucine for repression of the isoleucine-valine biosynthetic enzymes in Salmonella typhimurium. In contrast, the analogue had no effect on derepression of the leucine biosynthetic enzymes in leucine auxotrophs grown on limiting amounts of leucine. The effect of fluoroleucine on repression appeared to be specific for leucine since derepression of the isoleucine-valine enzymes due to an isoleucine or valine limitation was ...

The integration of donor and recipient deoxyribonucleic acid during transformation of Bacillus subtilis

1. DNA labelled with 5-bromo[3H]uracil was used to transform auxotrophic strains of Bacillus subtilis. 2. After various times of incubation, DNA was extracted from the transformed culture and subjected to equilibrium sedimentation in caesium chloride gradients. 3. In addition to heavy donor DNA and light recipient DNA, a component with an intermediate density was found and is believed to consist of a biological hybrid of donor and recipient. 4. The component of intermediate density was isolat...