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84617 documents, page 1 of 8462

The I-Ab mutant B6.C-H-2bm12 allows definition of multiple T cell epitopes on I-A molecules

The experiments presented in this study demonstrate that there exist at least two functional epitopes on an I-A molecule that can be recognized by T cell clones. By comparing the abilities of spleen cells from C57BL/6 mice and the congenic I-A mutant line B6.C-H-2bm12 to stimulate alloreactive T cell clones specific for the I-Ab molecule, we have discriminated two sets of clones, those recognizing the I-Ab and I- Abm12 molecule equally well and those able to recognize only the I-Ab molecule. ...

Improved preservation and staining of HeLa cell actin filaments, clathrin-coated membranes, and other cytoplasmic structures by tannic acid-glutaraldehyde-saponin fixation

Fixation of HeLa cells with a mixture of 100 mM glutaraldehyde, 2 mg/ml tannic acid and 0.5 mg/ml saponin allows the tannic acid to penetrate intact cells without disruption of membranes or extraction of the cytoplasmic matrix. After subsequent treatment with OsO4 cytoplasmic structures are stained so densely that fine details are visible even in very thin (dark gray) sections. Actin filaments are protected from disruption by OsO4 so that straight, densely stained filaments are seen in the ce...

Action of taxol on mitosis: modification of microtubule arrangements and function of the mitotic spindle in Haemanthus endosperm

We have studied the effect of taxol on mitosis in Haemanthus endosperm. Immuno-Gold Stain (IGS), a new immunocytochemical method (17), was used to visualize microtubules (MTs) in the light microscope. Observations on MT arrangements were correlated with studies in vivo. Chromosome movements are affected in all stages of mitosis which progresses over at least 10(4) range of taxol concentrations. The three most characteristic effects on MTs are: (a) enhancement of the lateral associations betwe...


Bone graftings were performed to two patients of cleft lip and palate. The first case was a 18-year-old female with nasal deformities, and was reconstructed using alveolar bone of the Г region. The second was a 14-year-old male with the floating premaxilla, and was reconstructed by Obwegeser's osteotomy of the premaxilla and fixation with grafted iliac bone. Satisfactory results were obtained for facial deformities and functional disorders of them, and usefulness of bone grafts to cleft lip a...