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144317 documents, page 1 of 14432

Regulation and activity-dependence of N-cadherin, NCAM isoforms, and polysialic acid on chick myotubes during development

(1993)
Muscle development in vivo involves a complex sequence of cell-cell interactions in which secondary myotubes first form in association with primary myotubes and subsequently separate from them. We show here that during this process N-cadherin and the different structural forms of NCAM are regulated in a pattern that involves both temporal changes in expression and localization to particular regions of the muscle cell surface. In particular, levels of N-cadherin on maturing myotubes are decrea...

Calcium influx mediated by the Escherichia coli heat-stable enterotoxin B (STB).

Dreyfus, L A; Harville, B; Howard, D E; Shaban, R; Beatty, D M; Morris, S J (1993)
The heat-stable enterotoxin B (STB) of Escherichia coli is a 48-amino acid extracellular peptide that induces rapid fluid accumulation in animal intestinal models. Unlike other E. coli enterotoxins that elicit cAMP or cGMP responses in the gut [heat-labile toxin (LT) and heat-stable toxin A (STA), respectively], STB induces fluid loss by an undefined mechanism that is independent of cyclic nucleotide elevation. Here we studied the effects of STB on intracellular calcium concentration ([Ca2+]i...

Excess intracellular concentration of the pSC101 RepA protein interferes with both plasmid DNA replication and partitioning.

Ingmer, H; Cohen, S N (1993)
RepA, a plasmid-encoded gene product required for pSC101 replication in Escherichia coli, is shown here to inhibit the replication of pSC101 in vivo when overproduced 4- to 20-fold in trans. Unlike plasmids whose replication is prevented by mutations in the repA gene, plasmids prevented from replicating by overproduction of the RepA protein were lost rapidly from the cell population instead of being partitioned evenly between daughter cells. Removal of the partition (par) locus increased the ...

Comparison of techniques for antimicrobial susceptibility testing of mycobacteria.

Limb, D I; Wheat, P F; Spencer, R C; Harris, G S; Rayner, A B; Watt, B (1993)
AIMS--To evaluate adenosine triphosphate (ATP) bioluminescence as a rapid technique for antimicrobial susceptibility testing of Mycobacterium spp by comparing it with conventional and radiometric methods, and to assess its potential for use in clinical microbiology laboratories. METHODS--115 clinical isolates from a wide range of mycobacterial species and four control organisms of known susceptibility were tested against six antimicrobial agents. Minimum inhibitory concentrations (MICs) were ...

Effects of relative plate motion on the deep structure and penetration depth of slabs below the Izu-Bonin and Mariana island arcs

Hilst, R.D. van der; Seno, Tetsuzo (1993)
An increasing number of seismological studies indicate that slabs of subducted lithosphere penetrate the Earth's lower mantle below some island arcs but are deflected, or, rather, laid down, in the transition zone below others. Recent numerical simulations of mantle flow also advocate a hybrid form of mantle convection, with intermittent layering. We present a multi-disciplinary analysis of slab morphology and mantle dynamics in which we account explicitly for the history of subduction below ...

Characterization of a novel chicken heat shock transcription factor, heat shock factor 3, suggests a new regulatory pathway.

Nakai, A; Morimoto, R I (1993)
We have cloned three avian heat shock transcription factor (HSF) genes corresponding to a novel factor, HSF3, and the avian homologs of mammalian HSF1 and HSF2. The predicted amino acid sequence of HSF3 is approximately 40% related to the sequence of HSF1 and HSF2. The sequences for all three factors exhibit extensive identify in the DNA binding motifs and the heptad repeats of hydrophobic amino acids which are common to all eukaryotic HSFs. Despite these overall similarities, each avian HSF ...

Elevated expression of basic fibroblast growth factor in an immortalized rabbit smooth muscle cell line.

Winkles, J. A.; Friesel, R.; Alberts, G. F.; Janat, M. F.; Liau, G. (1993)
Intimal smooth muscle cell accumulation is regarded as an important component of atherosclerotic plaque formation, angioplasty-induced restenosis, and vascular graft occlusion. Vascular smooth muscle cells can both express and respond to acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF); therefore, under certain conditions these polypeptides may regulate smooth muscle cell growth in an autocrine manner. Previous studies using smooth muscle cells cultured in vitr...