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Accessing the third dimension in scanning electron microscopy for rapid, high resolution tomography of large samples

Title
Accessing the third dimension in scanning electron microscopy for rapid, high resolution tomography of large samples
Funding
ARC | Linkage Infrastructure, Equipment and Facilities
Contract (GA) number
LE110100203
Start Date
2011/01/01
End Date
2011/12/31
Open Access mandate
no
Organizations
-
More information
http://purl.org/au-research/grants/arc/LE110100203

 

  • 3-D EM exploration of the hepatic microarchitecture – lessons learned from large-volume in situ serial sectioning

    Shami, Gerald John; Cheng, Delfine; Huynh, Minh; Vreuls, Celien; Wisse, Eddie; Braet, Filip (2016)
    Projects: ARC | Accessing the third dimension in scanning electron microscopy for rapid, high resolution tomography of large samples (LE110100203)
    To-date serial block-face scanning electron microscopy (SBF-SEM) dominates as the premier technique for generating three-dimensional (3-D) data of resin-embedded biological samples at an unprecedented depth volume. Given the infancy of the technique, limited literature is currently available regarding the applicability of SBF-SEM for the ultrastructural investigation of tissues. Herein, we provide a comprehensive and rigorous appraisal of five different SBF-SEM sample preparation protocols fo...
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