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Discovery Projects - Grant ID: DP110101580

Title
Discovery Projects - Grant ID: DP110101580
Funding
ARC | Discovery Projects
Contract (GA) number
DP110101580
Start Date
2011/01/01
End Date
2015/12/31
Open Access mandate
no
Organizations
-
More information
http://purl.org/au-research/grants/arc/DP110101580

 

  • A green fluorescent protein containing a QFG tri-peptide chromophore: optical properties and X-ray crystal structure.

    Jion M Battad; Daouda A K Traore; Emma Byres; Jamie Rossjohn; Rodney J Devenish; Seth Olsen; Matthew C J Wilce; Mark Prescott
    Projects: ARC | Discovery Projects - Grant ID: DP110101580 (DP110101580)
    Rtms5 is an deep blue weakly fluorescent GFP-like protein ([Formula: see text], 592 nm; [Formula: see text], 630nm; Φ(F), 0.004) that contains a (66)Gln-Tyr-Gly chromophore tripeptide sequence. We investigated the optical properties and structure of two variants, Rtms5(Y67F) and Rtms5(Y67F/H146S) in which the tyrosine at position 67 was substituted by a phenylalanine. Compared to the parent proteins the optical spectra for these new variants were significantly blue-shifted. Rtms5(Y67F) spectr...

    Valence-bond Non-equilibrium Solvation Model for a Twisting Monomethine Cyanine

    McConnell, Sean; McKenzie, Ross H.; Olsen, Seth (2014)
    Projects: ARC | Discovery Projects - Grant ID: DP110101580 (DP110101580)
    We propose and analyze a two-state valence-bond model of non-equilibrium solvation effects on the excited-state twisting reaction of monomethine cyanines. Suppression of this reaction is thought responsible for environment-dependent fluorescence yield enhancement in these dyes. Fluorescence is quenched because twisting is accompanied via the formation of dark twisted intramolecular charge-transfer (TICT) states. For monomethine cyanines, where the ground state is a superposition of structures...
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