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Publisher: Altai State University
Journal: Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)
Languages: Russian
Types: Article
Subjects: Epimedium Brevicornum, extraction with supercritical carbon dioxide, icariin, icariside II, epimedins A, B, C, high performance liquid chromatography, mass spectrometry, горянка коротконожковая; сверхкритическая экстракция диоксидом углерода; икариин; икаризид II; эпимедин A, В C; высокоэффективная жидкостная хроматография; масс-спектрометрия
Advantages of supercritical extraction in comparison with other extract methods for active components from plant materials were discussed. The possibility of utilization of extraction with supercritical carbon dioxide for the isolation of biologically active substances from the leaves of Epimedium Brevicornum was demonstrated. The dependence of efficiency of isolation of flavonoids from experimental conditions (temperature, pressure, extraction, time) was investigated. A maximal extraction yield of active components was obtained under optimal conditions: a temperature of 50 °С, a pressure of 30 MPa and an extraction time of 30 min. The comparison of the contents of icariin, icariside II, epimedins A, B, C in the leaves of Epimedium Brevicornum extracts obtained by extraction with supercritical carbon dioxide and by ultrasound liquid extraction procedures was carried out by of liquid chromatography/mass spectrometry. The superiority of proposed method to traditional ultrasound liquid-liquid extraction in 2,5 times was demonstrated.

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