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Куркин, Владимир Александрович; Акушская, Алина Сергеевна (2013)
Publisher: Altai State University
Journal: Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)
Languages: Russian
Types: Article
Subjects: ginseng, Panax ginseng C.A. Meyer, tincture, saponins, thin-layer chromatography, spectrophotometry, standardization, женьшень настоящий, Panax ginseng C.A.Meyer, настойка, сапонины, тонкослойная хроматография, спектрофотометрия, стандартизация
There were developed the techniques of qualitative and quantitative analysis of ginseng tinctures (Panax ginseng C.A. Meyer). Thin-layer chromatography (in the solvent system chloroform – methanol – water, 26 : 14 : 3; detection in the visible range after reaction with phosphowolframic acid) and UV/Visible spectroscopy of native tincture (maximum of absorption at 268±2 nm) and total saponins, purified on polyamide and reacted with 70% sulphuriс acid (maximum of absorption at 280±2 nm, 400±2 nm and 526±2 nm). The method of quantitative determination of total saponins in tinctura of ginseng by means of direct spectrophotometry (analytical wave-length at 526 nm) was developed. The contents of the total saponins are varied with 0,42% to 0,61% (calculated on ginsenoside Rg1)
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    • Received February 7, 2012
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