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Publisher: Altai State University
Journal: Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)
Languages: Russian
Types: Article
Subjects: толокнянка обыкновенная; Arctostaphylos uva-ursi (L.) Spreng.; арбутин; ВЭЖХ-анализ; спектрофотометрия; йодометрия; стандартизация., bearberry, Arctostaphylos uva-ursi (L.) Spreng., leaves, arbutin, HPLC-analysis, spectrophotometry, iodimetry, standardization
The method of quantitative determination of arbutin in leaves of bearberry (Arctostaphylos uva-ursi (L.) Spreng.) using reverse-phase HPLC and justified optimal conditions of extraction of arbutin from raw materials of plants (extractant – 40% ethyl alcohol; the extraction time – 60 min a boiling water bath; the ratio of «raw material : extractant» – 1 : 50). Conditions of carrying out of the analysis: the mobile phase 0,01 M KH2PO4 acidified Н3РО4 to pH 3,00±of 0,01, and acetonitrile (9 : 1); the flow of the mobile phase: 0,6 ml/min; detection at 280 nm. It is determined that the error of a single definition of the contents of arbutin in leaves of bearberry with confidence probability of 95% is +3,14%. The content of arbutin in leaves of bearberry varies from 10,85±0,24% to 11,16±0,35%.

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