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Publisher: Altai State University
Journal: Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)
Languages: Russian
Types: Article
Subjects: phenol-sulfuric acid method; renewable vegetable raw materials; polysaccharides; monosaccharides; lignin, возобновляемое растительное сырье; полисахариды;моносахариды; лигнин
Opportunities phenol-sulfuric acid method  for the analysis of vegetable raw materials are investigated. It is established that the method allows rather simply and with high precision to define polysaccharides as in vegetable raw materials (agricultural plants and softwood and hardwood), and in various preparations of a lignin (the laboratory-scale and technical). Color reaction of monosaccharides with phenol in the presence of the concentrated sulfuric acid is thebasis of a method. The developed modification phenol - sulfuric acid method is universal in the sense that allows to define polysaccharides in samples as with the high content of polysaccharides (vegetable raw materials), and with low (lignin preparations). The method possesses high sensitivity: concentration of monosaccharides in the mix prepared for the analysis averages 1×10-4 mol×l-1. For the analysis 0.25 cm3 of the hydrolyzate which is previously diluted by 10 times there are enough. Duration of the analysis of a hydrolyzate including mix preparation, record of aspectrum and calculation for a formula doesn't exceed  30 min. The method can be used for the analysis of a chemical composition of renewable vegetable raw materials when developing technologies of receiving alternative energy sources.
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