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Полуконова, Наталья; Дурнова, Наталья; Курчатова, Мария; Наволокин, Никита; Голиков, Алексей (2013)
Publisher: Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования «Алтайский государственный университет»
Journal: Химия растительного сырья
Languages: Russian
Types: Article
Subjects: Sammlung Dietmar Brandes [Digitale Pflanzenbilder], Chemistry, Q, авран лекарственный, химический состав, биофлавоноиды, способ экстракции, кверцетин, QD1-999, АВРАН ЛЕКАРСТВЕННЫЙ,ХИМИЧЕСКИЙ СОСТАВ,БИОФЛАВОНОИДЫ,СПОСОБ ЭКСТРАКЦИИ,КВЕРЦЕТИН, Science, Gratiola officinalis L., hyssop, bioflavonoids, chemical composition, extraction method, Quercetin, ЭКСТРАКТ АВРАНА ЛЕКАРСТВЕННОГО, GRATIOLA OFFICINALIS L., ФЛАВОНОИДЫ, ПРОПИДИЙ ЙОДИСТЫЙ, КЛЕТКИ ПОЧЕК ЭМБРИОНОВ СВИНЬИ (SPEV-2), АПОПТОЗ
ddc: ddc:580, ddc:58, ddc:5
Active dry extract solution Gratiola officinalis L. investigated on pigs embryo kidney cells in culture SPEV-2 using propidium iodide (PI). Concentration of 32 and 320 ug/ml had a cytostatic effect on cells SPEV-2. Concentrations of 3,2 and 32 mg/ml had a cytotoxic effect. During the day the extract induced apoptosis in cells at concentrations of 32 and 320 ug/ml. Overlaying still images allowed us to describe in different microscopic scattering and fluorescence registration, cytomorphological parameters under the influence of the extract, marking process of cell death. These indicators reflect the DNA damage in cells under conditions in vitro. We described the following indicators: the appearance of dark structures in the cytoplasm, indistinct outlines of the cells themselves and their acquisition of star-shaped or rounded preservation, but the fragmentation of the cell membrane and spillage; formation of conglomerates dead cells, Reducing the emission intensity of propidium iodide indicates the decay of the nucleus DNA. Index numbers of dead cells (CMC) can be used an index of cytotoxicity.