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Базарнова (Bazarnova), Наталья (Natal'ia) Григорьевна (Grigor'evna); Ильичёва (Il'icheva), Татьяна (Tat'iana) Николаевна (Nikolaevna); Тихомирова (Tikhomirova), Людмила (Liudmila) Ивановна (Ivanovna); Синицина (Sinitsyna), Анастасия (Anastasiia) Александровна (Aleksandrovna) (2016)
Publisher: Altai State University
Journal: Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)
Languages: Russian
Types: Article
Subjects: I. sibirica L.; medicinal plant raw material; screening of chemical composition; biological activity; grade Cambridge; the herpes simplex virus, Iris sibirica L.; лекарственное растительное; скрининг химического состава; биологическая активность; сорт Cambridge; вирус простого герпеса
The aim of this work was to conduct an initial analysis of the chemical composition and biological activity of raw materials I. sibirica L. grade Cambridge spring and fall collection. Identification of key biologically active components of Siberian iris is made in accordance with the recommendation of Muzychkina R. A. with colleagues. To analyze antiviral activity in vitro used method for measuring absorption in vivo by cells of the dye neutral red (NC). As a result of successive extraction of raw materials I. sibirica different solvents established that the leaves and rhizomes contain a minimum fraction extractable with chloroform, and the dominant fraction was extracted with water, regardless of the timing of raw material collection (spring, fall). The maximum value of content of extractive substances extracted with hexane and chloroform were determined in leaves of spring flush within 4% for and.with.in. The content of extractives allocated 96% ethanol, the maximum was determined in the leaves of the spring collection – 7,51%, the minimum value detected in the rhizomes of the autumn collection – 1,91%. All the samples were found flavonoids, tannins, glycosides, phenolic acids, coumarins, xanthones, saponins, terpenes. Detected in leaves glucose and galactose, and in the rhizomes of arabinose, glucose, galactose.For the first time determined the biological activity of extracts of I. sibirica raw material against the herpes viruses. The selected samples are promising for further studies in animal experiments.Further studies of the chemical composition of raw materials I. sibirica L. with the aim of determining the quantitative composition of biologically active substances. To determine the biological activity of extracts against influenza viruses.
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