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Shivanna, Gunashree B.; Govindarajulu, Venkateswaran (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article

Classified by OpenAIRE into

mesheuropmc: fungi
Objective: Aspergillus niger is one of the fungi exploited industrially for the production of various metabolites including the enzyme phytase. Intensive sporulation is a serious crisis in using this fungus, hence, in the present study an attempt was made to obtain an asporulating strain.Materials and methods: A. niger CFR 335, a local phytase-producing isolate, was induced to physical mutation by ultraviolet (UV) radiation and chemical mutation using different concentrations of ethyl methane sulphonate (EMS) and N-methyl N?-nitro N-nitrosoguanidine (NTG).Results: UV exposure for 30 min, 10 mM EMS and 25 ug ml^-1 of NTG were found to be optimum for maximum killing effect of 26.3%, 95% and 80% of the spores, respectively. In this experiment, a stable auxotrophic mutant (ala) of A. niger CFR 335 with suppressed sporulation was obtained. The auxotrophic mutant M-1 (A. niger CFR 335 ala) showed an improved specific activity of 1.26 U mg^-1 as compared with the parent strain (0.85 U mg1).Conclusion: Sporulation was drastically reduced in the mutant M-1 without affecting phytase yield, whereas the production rate was proportional to the rate of sporulation in the parent strain (A. niger CFR 335).Key words: asporulation, Aspergillus niger CFR 335, phytase, ethyl methane sulphonate, N- methyl N?-nitro N-nitrosoguanidine, ultraviolet radiation
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    • 1. Suzuki M, Tanaka K, Kuwano M, Yoshida KT. Expression pattern of inositol phosphate-related enzymes in rice (Oryza sativa L.): implications for the phytic acid biosynthetic pathway. Gene. 2007;/405:/55 64.
    • 2. Piddington CS, Houston CS, Paloheimo M, Cantrell M, Miettinen-Oinonen A, Nevalainen H, et al. The cloning and sequencing of the genes encoding phytase (phy) and pH 2.5- optimum acid phosphatase (aph) from Aspergillus niger var. awamori. Gene. 1993;/133:/55 62.
    • 3. Haddad J, Greiner R, Allaf K. Effect of instantaneous controlled pressure drop on the phytate content of lupin. LWT Food Sci Technol. 2007;/40:/448 53.
    • 4. Grases F, Perello J, Prieto RM, Simonet BM, Torres JJ. Dietary myo-inositol hexaphosphate prevents dystrophic calcifications in soft tissues: a pilot study in Wistar rats. Life Sci. 2004;/75:/11 9.
    • 5. Pagano AR, Yasuda K, Roneker KR, Crenshaw TD, Lei XG. Supplemental Escherichia coli phytase and strontium enhance bone strength of young pigs fed a phosphorus- adequate diet. J Nutr. 2007;/137:/1795 801.
    • 6. Reddy NR, Sathe SK, Salunkhe DK. Phytates in legumes and cereals. Adv Food Res. 1982;/28:/1 92.
    • 7. Wang C, Eufemi M, Turano C, Giartosio A. Influence of the carbohydrate moiety on the stability of glycoproteins. Biochemistry. 1996;/35:/7299 307.
    • 8. Mitchell DB, Vogel K, Weimann B, Pasamontes L, van Loon APGM. The phytase subfamily of histidine acid phosphatases: isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila. Microbiology. 1997;/143:/245 52.
    • 9. Hara S, Kitamoto K, Gomi K. New developments in fermented beverages and foods with Aspergillus. In: Bennett JW, Klich MA. editors. Aspergillus: biology and industrial applications. Boston: Butterworth-Heinemann; 1992. p. 133.
    • 10. Banerjee AC, Kundu A, Ghosh SK. Genetic manipulation of filamentous fungi. In: Roussos S, editor. New horizons in biotechnology. Dordrecht, The Netherlands: Kluwer Academic Publishers; 2003. p. 193 8.
    • 11. Gunashree BS, Venkateswaran G. Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations. J Ind Microbiol Biotechnol. 2008;/35:/1587 96.
    • 12. Omogbenigun FO, Nyachoti CM, Slominski BA. The effect of supplementing microbial phytase and organic acids to a corn-soybean based diet fed to early-weaned pigs. J Anim Sci. 2003;/81:/1806 13.
    • 13. Sariyska MV, Gargova SA, Koleva LA, Angelov AI. Aspergillus niger phytase: purification and characterization. Biotechnol Biotechnol Eq. 2005;/19:/98 105.
    • 14. Ha NC, Kim YO, Oh TK, Oh BH. Preliminary X-ray crystallographic analysis of a novel phytase from a Bacillus amyloliquefaciens strain. Acta Crystallogr D Biol Crystallogr. 1999;/55:/691 3.
    • 15. Ehrlich KC, Montalbano BG, Mullaney EJ, Dischinger HC, Ullah AHJ. Identification and cloning of a second phytase gene (Phyt B) from Aspergillus niger (ficuum). Biochem Biophys Res Commun. 1993;/195:/53 6.
    • 16. Hideaki H, Shigeru T, Hayato K, Takashi S, Hirotsugu T, Akihisa F, et al. Allergic bronchopulmonary aspergillosis due to Aspergillus niger without bronchial asthma. Respiration. 1999;/66:/369 72.
    • 17. Mu¨ ller A, Lehmann I, Seiffart A, Diez U, Wetzig H, Bort M, et al. Increased incidence of allergic sensitisation and respiratory diseases due to mould exposure: results of the Leipzig Allergy Risk children Study (LARS). Int J Hyg Environ Health. 2002;/204:/363 5.
    • 18. Howson SJ, Davis RP. Production of phytate-hydrolyzing enzyme by some fungi. Enzyme Microbiol Technol. 1983;/5:/ 377 82.
    • 19. Usha T, Venkateshwaran G, Sarada R, Ravishankar GA. Studies on Haematococcus pluvialis for improved production of astaxanthin by mutagenesis. World J Microbiol Biotechnol. 2001;/17:/143 8.
    • 20. Venkateswaran G. Lipid modification in Rhodotorula gracilus: biochemical and genetic studies. PhD thesis, University of Mysore, India, 1997.
    • 21. Holliday RA. New method for the identification of biochemical mutants of microorganisms. Nature. 1956;/178:/987.
    • 22. Heinonen JK, Lahti RJ. A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatases. Anal Biochem. 1981;/113:/313 7.
    • 23. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal of protein dye binding. Anal Biochem. 1976;/72:/ 248 54.
    • 24. SAS Institute. SAS/STAT guide or personal computers. Ver. 6.03. SAS Inst., Cary, NC, 1988.
    • 25. Rodriguez E, Wood ZA, Karpus PA, Lie XG. Site-directed mutagenesis improves catalytic efficiency and thermostability of Escherichia coli pH 2.5 acid phosphatase/phytase expressed in Pichia pastoris. Arch Biochem Biophys. 2000;/382:/105 12.
    • 26. Gu WN, Yang PL, Wang YR, Luo HY, Meng K, Wu NF, et al. Mutation research on Q23L and Q23LG272E in phytase derivated from Aspergillus fumigatus. Sheng Wu Gong Cheng Xue Bao. 2007;/23:/273 7.
    • 27. Mullaney EJ, Daly CB, Kim T, Porres JM, Lei XG, Sethumadhavan K. Site-directed mutagenesis of Aspergillus niger NRRL 3135 phytase at residue 300 to enhance catalysis at pH 4.0. Biochem Biophys Res Commun. 2002;/297:/ 1016 20.
    • 28. Chelius MK, Wodzinski RJ. Strain improvement of Aspergillus niger for phytase production. Appl Microbiol Biotechnol. 1994;/41:/78 83.
    • 29. Petter M, Schnu¨ rer J, Gerhart E, Wagner H. Characterization of phiA, a gene essential for phialide development in Aspergillus nidulans. Fungal Genet Biol. 2003;/40:/234 41.
    • 30. Teepe AG, Loprete DM, He Z, Hoggard TA, Hill TW. The protein kinase C orthologue PkcA plays a role in cell wall integrity and polarized growth in Aspergillus nidulans. Fungal Genet Biol. 2007;/44:/554 62.
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