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Reid, Gregor (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article

Classified by OpenAIRE into

mesheuropmc: food and beverages
The association between depletion of vaginal lactobacilli and increased risk of various urogenital infections and complications such as preterm labor, has been known for some time. Restoration of depleted lactobacilli by administration of probiotic strains has been demonstrated in humans using Lactobacillus rhamnosus GR-1 and L. reuteri RC-14. These organisms also augment antibiotic efficacy, lessen the side effects of these drugs, and alleviate diarrhea in AIDS patients. The mechanisms appear to be multi-factorial, and include production of: (i) anti-microbial factors such as lactic acid, bacteriocins, hydrogen peroxide, (ii) biosurfactants or other components that affect pathogen colonization and biofilm formation, (iii) signalling compounds that influence pathogen virulence expression, and (iv) signalling compounds that modulate immunity. Recombinant strains have been produced that inhibit or kill HIV, offering hope for microbicide applications that can be self-used by women. As more information becomes available about the ‘normal’ versus ‘disease prone’ vaginal microbiota and strains that confer the most benefits, current and new probiotic remedies will potentially provide improved restorative and therapeutic options to lower the one billion urogenital infections currently afflicting women around the world.Key words: probiotics, lactobacillus, urogenital infections, mechanisms
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1. Boyd MA, Antonio MA, Hillier SL. Comparison of API 50 CH strips to whole-chromosomal DNA probes for identification of Lactobacillus species. J Clin Microbiol. 2005;/43(10):/ 5309 11.
    • 2. Burton JP, Cadieux PA, Reid G. Improved understanding of the bacterial vaginal microbiota of women before and after probiotic instillation. Appl Environ Microbiol. 2003;/69(1):/ 97 101.
    • 3. Vasquez A, Jakobsson T, Ahrne S, Forsum U, Molin G. Vaginal Lactobacillus flora of healthy Swedish women. J Clin Microbiol. 2002;/40(8):/2746 9.
    • 4. Devillard E, Burton JP, Hammond JA, Lam D, Reid G. Novel insight into the vaginal microflora in postmenopausal women under hormone replacement therapy as analyzed by PCRdenaturing gradient gel electrophoresis. Eur J Obstet Gynecol Reprod Biol. 2004;/117(1):/76 81.
    • 5. Burton JP, Devillard E, Cadieux PA, Hammond JA, Reid G. Detection of Atopobium vaginae in postmenopausal women by cultivation-independent methods warrants further investigation. J Clin Microbiol. 2004;/42(4):/1829 31.
    • 6. Verstraelen H, Verhelst R, Claeys G, Temmerman M, Vaneechoutte M. Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosis. Am J Obstet Gynecol. 2004;/191(4):/ 1130 2.
    • 7. Fredricks DN, Fiedler TL, Marrazzo JM. Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med. 2005;/353(18):/1899 911.
    • 8. Heinemann C, Reid G. Vaginal microbial diversity among postmenopausal women with and without hormone replacement therapy. Can J Microbiol. 2005;/51(9):/777 81.
    • 9. Raz R, Stamm WE. A controlled trial of intravaginal estriol in postmenopausal women with recurrent urinary tract infections. N Engl J Med. 1993;/329(11):/753 6.
    • 10. Tao L, Pavlova SI. Images in infectious diseases in obstetrics and gynecology. Vaginal Lactobacillus phage plaques and electron micrograph. Infect Dis Obstet Gynecol. 1998;/6(6):/ 236.
    • 11. Kelly MC, Mequio MJ, Pybus V. Inhibition of vaginal lactobacilli by a bacteriocin-like inhibitor produced by Enterococcus faecium 62 6: potential significance for bacterial vaginosis. Infect Dis Obstet Gynecol. 2003;/11(3):/147 56.
    • 12. Reid G, Beuerman D, Heinemann C, Bruce AW. Probiotic Lactobacillus dose required to restore and maintain a normal vaginal flora. FEMS Immunol Med Microbiol. 2001;/32(1):/ 37 41.
    • 13. Guerra B, Ghi T, Quarta S, Morselli-Labate AM, Lazzarotto T, Pilu G, Rizzo N. Pregnancy outcome after early detection of bacterial vaginosis. Eur J Obstet Gynecol Reprod Biol. 2006 Feb 2.
    • 14. Sewankambo N, Gray RH, Wawer MJ, et al. HIV-1 infection associated with abnormal vaginal flora morphology and bacterial vaginosis. Lancet. 1997;/350(9077):/546 50.
    • 15. Wiesenfeld HC, Hillier SL, Krohn MA, Landers DV, Swet RL. Bacterial vaginosis is a strong predictor of Neisseria gonorroeae and Chlamydia trachomatis infection. Clin Infect Dis. 2003;/36:/663 8.
    • 16. Schoeman J, Steyn PS, Odendaal HJ, Grove D. Bacterial vaginosis diagnosed at the first antenatal visit better predicts preterm labour than diagnosis later in pregnancy. J Obstet Gynaecol. 2005;/25(8):/751 3.
    • 17. Reid G, Cook RL, Bruce AW. Examination of strains of lactobacilli for properties that may influence bacterial interference in the urinary tract. J Urol. 1987;/138(2):/330 5.
    • 18. Bruce AW, Reid G. Intravaginal instillation of lactobacilli for prevention of recurrent urinary tract infections. Can J Microbiol. 1988;/34(3):/339 43.
    • 19. Zhong W, Millsap K, Bialkowska-Hobrzanska H, Reid G. Differentiation of Lactobacillus species by molecular typing. Appl Environ Microbiol. 1998;/64(7):/2418 23.
    • 20. FAO/WHO. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Food and Agriculture Organization of the United Nations and World Health Organization Expert Consultation Report. 2001. http:// www.fao.org/es/ESN/Probio/probio.htm
    • 21. Reid G, Bruce AW, Taylor M. Instillation of Lactobacillus and stimulation of indigenous organisms to prevent recurrence of urinary tract infections. Microecol Ther. 1995;/23:/32 45.
    • 22. Reid G, Millsap K, Bruce AW. Implantation of Lactobacillus casei var rhamnosus into the vagina. The Lancet. 1994;/344:/ 1229.
    • 23. Kontiokari T, Sundqvist K, Nuutinen M, Pokka T, Koskela M, Uhari M. Randomised trial of cranberry-lingonberry juice and Lactobacillus GG drink for the prevention of urinary tract infections in women. BMJ. 2001;/322(7302):/1571.
    • 24. Colodner R, Edelstein H, Chazan B, Raz R. Vaginal colonization by orally administered Lactobacillus rhamnosus GG. Isr Med Assoc J. 2003;/5(11):/767 9.
    • 25. Baerheim A, Larsen E, Digranes A. Vaginal application of lactobacilli in the prophylaxis of recurrent lower urinary tract infection in women. Scand J Prim Health Care. 1994;/12(4):/ 239 43.
    • 26. Pirotta M, Gunn J, Chondros P, Grover S, O'Malley P, Hurley S, Garland S. Effect of Lactobacillus in preventing post-antibiotic vulvovaginal candidiasis: a randomised controlled trial. BMJ. 2004;/329(7465):/548.
    • 27. Eschenbach DA, Thwin SS, Patton DL, Hooton TM, Stapleton AE, Agnew K, Winter C, Meier A, Stamm WE. Influence of the normal menstrual cycle on vaginal tissue, discharge, and microflora. Clin Infect Dis. 2000;/30(6):/901 7.
    • 28. Chan RC, Bruce AW, Reid G. Adherence of cervical, vaginal and distal urethral normal microbial flora to human uroepithelial cells and the inhibition of adherence of gramnegative uropathogens by competitive exclusion. J Urol. 1984;/131(3):/596 601.
    • 29. Huff BA. Caveat emptor. ''Probiotics'' might not be what they seem. Can Fam Physician. 2004;/50:/583 7.
    • 30. Reid G, Hammond JA. Beware of ''natural'' products. Can Fam Physician. 2004;/50:/1081.
    • 31. Reid G. Food and Agricultural Organization of the United Nation and the WHO. The importance of guidelines in the development and application of probiotics. Curr Pharm Des. 2005;/11(1):/11 6.
    • 32. Reid G, Hammond J-A. Probiotics:some evidence of their effectiveness. Can Fam Phys. 2005;/51:/1487 93.
    • 33. Reid G, Devillard E. Probiotics for the mother and child. J Clin Gastroenterol. 2004;/38(Suppl. 2):/S94 101.
    • 34. Cadieux P, Burton J, Kang CY, Gardiner G, Braunstein I, Bruce AW, Reid G. Lactobacillus strains and vaginal ecology. JAMA. 2002;/287:/1940 1.
    • 35. Morelli L, Zonenenschain D, Del Piano M, Cognein P. Utilization of the intestinal tract as a delivery system for urogenital probiotics. J Clin Gastroenterol. 2004:38(6 Suppl):S107 10.
    • 36. Reid G, Charbonneau D, Erb J, Kochanowski B, Beuerman D, Poehner R, Bruce AW. Oral use of Lactobacillus rhamnosus GR-1 and L. fermentum RC-14 significantly alters vaginal flora: randomized, placebo-controlled trial in 64 healthy women. FEMS Immunol Med Microbiol. 2003;/35(2):/131 4.
    • 37. Reid G, Burton J, Hammond JA, Bruce AW. Nucleic acidbased diagnosis of bacterial vaginosis and improved management using probiotic lactobacilli. J Med Food. 2004 Summer;/ 7(2):/223 8.
    • 38. Anukam KC, Osazuwa E, Osemene GI, Ehigiagbe F, Bruce AW, Reid G. Clinical study comparing probiotic Lactobacillus GR-1 and RC-14 with metronidazole vaginal gel to treat symptomatic bacterial vaginosis. Microbes Infect. 2006; 8(12 13):2772 76.
    • 39. Chang TL, Chang CH, Simpson DA, Xu Q, Martin PK, Lagenaur LA, Schoolnik GK, Ho DD, Hillier SL, Holodniy M, Lewicki JA, Lee PP. Inhibition of HIV infectivity by a natural human isolate of Lactobacillus jensenii engineered to express functional two-domain CD4. Proc Natl Acad Sci USA. 2003;/100(20):/11672 7.
    • 40. Pusch O, Boden D, Hannify S, Lee F, Tucker LD, Boyd MR, Wells JM, Ramratnam B. Bioengineering lactic acid bacteria to secrete the HIV-1 virucide cyanovirin. J Acquir Immune Defic Syndr. 2005;/40(5):/512 20.
    • 41. Liu JJ, Reid G, Jiang Y, Turner MS, Tsai C-C. Expression of HIV-inactivating proteins by recombinant human vaginal probiotic Lactobacillus reuteri RC-14. Cell. Microbiol. 2006; online Jul 31.
    • 42. Reid G, Anand S, Bingham MO, Mbugua G, Wadstrom T, Fuller R, Anukam K, Katsivo M. Probiotics for the developing world. J Clin Gastroenterol. 2005;/39(6):/485 8.
    • 43. Cummins J, Ho M-W. Genetically modified probiotics should be banned. Microbial Ecol. Health Dis. 2005;/17:/66 8.
    • 44. Steidler L. Delivery of therapeutic proteins to the mucosa using genetically modified microflora. Expert Opin Drug Deliv. 2005;/2(4):/737 46.
    • 45. Beigi RH, Wiesenfeld HC, Hillier SL, Straw T, Krohn MA. Factors associated with absence of H2O2-producing Lactobacillus among women with bacterial vaginosis. J Infect Dis. 2005;/191(6):/924 9.
    • 46. Valore EV, Park CH, Igreti SL, Ganz T. Antimicrobial components of vaginal fluid. Am J Obstet Gynecol. 2002;/ 187(3):/561 8.
    • 47. Boskey ER, Cone RA, Whaley KJ, Moench TR. Origins of vaginal acidity: high D/L lactate ratio is consistent with bacteria being the primary source. Hum Reprod. 2001;/ 16(9):/1809 13.
    • 48. Velraeds MM, van de Belt-Gritter B, van der Mei HC, Reid G, Busscher HJ. Interference in initial adhesion of uropathogenic bacteria and yeasts to silicone rubber by a Lactobacillus acidophilus biosurfactant. J Med Microbiol. 1998;/47(12):/ 1081 5.
    • 49. Millsap K, Reid G, van der Mei HC, Busscher HJ. Displacement of Enterococcus faecalis from hydrophobic and hydrophilic substrata by Lactobacillus and Streptococcus spp. as studied in a parallel plate flow chamber. Appl Environ Microbiol. 1994;/60(6):/1867 74.
    • 50. Reid G, Tieszer C. Preferential adhesion of bacteria from a mixed population to a urinary catheter. Cells Materials. 1993;/ 3:/171 6.
    • 51. Laughton JM, Devillard E, Heinrichs DE, Reid G, McCormick JK. Inhibition of expression of a staphylococcal superantigen-like protein by a soluble factor from Lactobacillus reuteri . Microbiology. 2006;/152(Pt 4):/1155 67.
    • 52. Gan BS, Kim J, Reid G, Cadieux P, Howard JC. Lactobacillus fermentum RC-14 inhibits Staphylococcus aureus infection of surgical implants in rats. J Infect Dis. 2002;/185(9):/1369 72.
    • 53. Ding W, Wang H, Griffiths MW. Probiotics down-regulate flaA sigma28 promoter in Campylobacter jejuni . J Food Prot. 2005;/68(11):/2295 300.
    • 54. Pessi T, Sutas Y, Hurme M, Isolauri E. Interleukin-10 generation in atopic children following oral Lactobacillus rhamnosus GG. Clin Exp Allergy. 2000;/30(12):/1804 8.
    • 55. Kim SO, Sheik HI, Ha S-D, Martins A, Reid G. G-CSF mediated inhibition of JNK is a key mechanism for Lactobacillus rhamnosus -induced anti-inflammatory effects in macrophages. Cell. Microbiol. 2006; online Aug. 2; Nov 6;8(12): 1958 71.
    • 56. Ma D, Forsythe P, Bienenstock J. Live Lactobacillus reuteri is essential for the inhibitory effect on tumor necrosis factor alpha-induced interleukin-8 expression. Infect Immun. 2004;/ 72(9):/5308 14.
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