LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Adawi, Diya; Molin, Göran; Ahrné, Siv; Jeppsson, Bengt (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
Subjects:

Classified by OpenAIRE into

mesheuropmc: fluids and secretions, food and beverages
Objective: To evaluate the effects of the administration of different bacterial strains on the extent of liver injury and bacterial translocation in an acute liver injury model. Design: Experimental study. Setting: University hospital, Sweden. Subjects: Sprague–Dawley rats. Interventions: Six different bacterial strains (Bacteroides fragilis ATCC 25285T, Enterococcus faecium No.1, Enterococcus faecium No.2, Escherichia coli F131, Lactobacillus plantarum DSM 6595, and Bifidobacterium longum ATCC 15707T) were administered rectally daily, for 8 days. Liver injury induced on the 8th day by intraperitoneal injection of D-galactosamine (1.1 g/kg BW). Main outcome measures: Samples were collected 24 h after the liver injury. Liver enzymes and bilirubin serum levels, bacterial translocation (to arterial and portal blood, liver and mesenteric lymph nodes), and intestinal microflora were evaluated. Results: The incidence of bacterial translocation to arterial and portal blood decreased significantly in Lb. plantarum and Bd. longum groups compared to the liver injury. The number of translocated bacteria to portal blood decreased in Bd. longum, Lb. plantarum, Ent. Faecium No.1, Ent. Faecium No.2 groups compared to E. coli group. In the arterial blood it decreased in Lb. plantarum and B. longum groups compared to Bact. fragilis and E. coli groups. Bacterial translocation to the liver increased significantly in E. coli group compared to liver injury, while it decreased in Lb. plantarum and Bd. longum groups compared to liver injury, Bact. fragilis and E. coli groups. In mesenteric lymph nodes, bacterial translocation decreased in Lb. plantarum group compared to liver injury, Bact. fragilis and E. coli groups. The release of liver enzymes increased in Bact. fragilis group compared to all the other groups. It decreased in Lb. plantarum compared to liver injury, Bact. fragilis and E. coli groups. Conclusion: Modulation of the intestinal microflora by different bacterial types has different effects on the extent of liver injury and bacterial translocation. Administration of Bact. fragilis and E. coli increased bacterial translocation and the extent of the liver injury. Administration of Lb. plantarum and Bd. longum reduced bacterial translocation, while Lb. plantarum reduced hepatocyte damage indicated by decreased liver enzyme release.Keywords: liver injury, bacterial translocation, D-alactosamine, intestinal microflora, Lactobacillus plantarum.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1. Bismuth H, Samuel D, Gugenheim J. Emergency liver transplantation for fulminant hepatitis. Ann Intern Med 1987; 107: 337 - 41.
    • 2. Rakela J, Lange SM, Ludwig J. Fulminant hepatitis: Mayo Clinics experience with 34 cases. Mayo Clin Proc 1985; 60: 289 - 92.
    • 3. Gardiner KR, Erwin PJ, Anderson NH, Barr JG, Halliday MI, Rowlands BJ. Colonic bacteria and bacterial translocation in experimental colitis. Br J Surg 1993; 80: 512 - 6.
    • 4. Walker WA. Role of the mucosal barrier in toxin:microbial attachment to the gastrointestinal tract. In: Microbiol Toxins and Diarrhoeal Disease. Ciba Foundation Symposium 112. London: Pitman, 1985: 34 - 47.
    • 5. van der Waiij D. Colonization resistance of the digestive tract - mechanisms and clinical consequences. Nahrung 1987; 31: 507 - 17.
    • 6. Ma L, Deitch E, Spcian R, Steffen E, Berg R. Translocation of Lactobacillus murinus from the Gastrointestinal Tract. Curr Microbiol 1990; 20: 177 - 84.
    • 7. Fernandes CF, Shahani KM, Amer MA. Therapeutic role of dietary lactobacilli and lactobacillic fermented diary products. FEMS Microbiol Rev 1987; 46: 343 - 56.
    • 8. Wells CL, Maddaus MA, Reynolds CM, Jechorek RP, Simmons RL. Role of anaerobic flora in the translocation of aerobic and facultative anaerobic intestinal bacteria. Infect Immun 1987; 55: 2689 - 94.
    • 9. Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota: Introducin the concept of prebiotics. J Nutr 1995; 125: 1401 - 12.
    • 10. Goldstein EJ. Anaerobic bacteremia. Clin Infect Dis 1996; 23 (Suppl 1): S97 - 101.
    • 11. Wade JJ. Enterococcus faecium in hospitals. Eur J Clin Microbiol Infect Dis 1997; 16 (2): 113 - 9.
    • 12. Moellering RC Jr. Vancomycin resistant enterococci. Clin Infect Dis 1998; 26 (5): 1196 - 9.
    • 13. De-Vera ME. Antibiotic resistant enterococci and the changing face of surgical infections. Arch Surg 1996; 131 (3): 338 - 42.
    • 14. Molin F, Jeppsson B, Ahrne S, et al. Numerical taxonomy of Lactobacillus spp. Associated with healthy and diseased mucosa of the human intestines. J App Bacteriol 1993; 74: 314 - 23.
    • 15. Adawi D, Kasravi FB, Molin G, Jeppsson B. Effect of Lactobacillus administration with and without arginine on liver damage and bacterial translocation in an acute liver injury model in the rat. Hepatology 1997; 25: 642 - 7.
    • 16. Sommer BG, Sutherland DER, Matas AJ, Simmons RL, Najarian JS. Hepatocellular transplantation for treatment of D-galactosamine induced acute liver failure in rats. Transplant Proc 1979; 11 (1): 578 - 84.
    • 17. Johansson ML, Molin G, Jeppsson B, Nobaek S, Ahrne S, Bengmark S. Administration of different Lactobacillus strains in fermented oatmeal soap. Appl & Environ Microbiol 1993; 59: 15 - 20.
    • 18. The Committee on Enzymes of the Scandinavian Society for Clinical Chemistry and Clinical Physiology. Recommended method for the determination of four enzymes in the blood. Scand J Clin Lab Inves 1974; 33: 291 - 306.
    • 19. Godfrey K. Comparing the means of several groups. In: Bailar III JC, Mosteller F, eds. Medical uses of statistics. Waltham, Massachusetts USA: New England Journal of Medicine Books, 1986: 205 - 34.
    • 20. Billiar T, Maddaus M, West M, et al. Intestinal gram-negative bacterial overgrowth in vivo augments the in vitro response of Kupffer cells to endotoxin. Ann Surg 1988; 208: 532 - 40.
    • 21. Billiar T, Maddaus M, West M, et al. The role of intestinal flora on the interaction between nonparenchynal cells and hepatocytes in coculture. J Surg Res 1988; 44: 397 - 403.
    • 22. Johansson M-L, Quednau M, Ahrne S, Molin G. Classification of Lactobacillus plantarum by restriction endonuclease analysis of total chromosomal DNA using conventional agarose gel electrophoresis. Int J Syst Bacteriol 1995; 45: 670 - 5.
    • 23. Rowland IR, Rumney CJ, Coutts JT, Lievense LC. Effect of Bifidobacterium longum and inulin on gut bacterial metabolism and carcinogen-induced aberrant crypt foci in rats. Carcinogenesis. 1998; 19 (2): 281 - 5.
    • 24. Holdeman LV, Holdeman RW, Moore EC. (1984) Anaerobic Gram-negative straight curved and helical rods. pp. 602 - 662. In: Bergey's Manual of Systemic Bacteriology. Vol. 1. Eds.: Krieg NR and Holt JG. Williams & Wilkins, Baltimore.
    • 25. Marshall JC, Christou NV, Meakins JL. Immunomodlation by altered gastrointestinal tract flora. Arch Surg 1988; 123: 1465 - 9.
    • 26. Marshall JC, Christou NV, Meakins JL. The gastrointestinal tract: The ''undrained abscess'' of multiple organ failure. Ann Surg 1993; 218 (2): 111 - 9.
    • 27. Mao Y, Yu J-L, Ljungh A˚ , Molin G, Jeppsson B. Intestinal immune response to oral administration of Lactobacillus reuteri R2LC, Lactobacillus DSM 9843, pectin and oatbase on methotrexate-induced enterocolitis in rats. Microbial Ecol Health Dis 1996; 9: 261 - 70.
    • 28. Mao Y, Nobaek S, Kasravi B, et al. The effects of Lactobacillus strains and oat fiber on methotrexate-induced enterocolitis in rats. Gastroenterology 1996; 111: 334 - 44.
    • 29. Van-Leeuwen PA, Boemeester MA, Houdijk AP, et al. Clinical significance of translocation. Gut 1994; 35 (supp): 328 - 34.
    • 30. Berg RD, Garlington AW. Translocation of certain indigenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and other organs in a gnotobiotic mouse model. Infect Immun 1979; 23 (2): 403 - 11.
    • 31. Berg RD, Wommack E, Deitch RA. Immunosuppression and intestinal bacterial overgrowth synergistically promote bacterial translocation. Arch Surg 1988; 123: 1359 - 64.
    • 32. Hughes RD, Wendon J, Gimson AES. Acute liver failure. Gut Supp 1991; S86 - S91.
    • 33. Canalese J, Gove CD, Gimson AES, Wilkinson SP, Wardle EN, Williams R. Reticuloendothelial system and hepatocyte function in fulminant hepatic failure. Gut 1982; 23: 265 - 9.
    • 34. Kasravi FB, Wang L, Wang X, Molin G, Bengmark S, Jeppsson B. Bacterial translocation in acute liver injury induced by D-galactosamine. Hepatology 1996; 23: 97 - 103.
    • 35. Kasravi FB, Wang X, Andersson R, Norgren L, Jeppsson B, Bengmark S. Reticuloendothelial system function in acute liver injury induced by D-galactosamine. J Hepatol 1995; 727 - 33.
    • 36. Wells CL. Relationship between intestinal microecology and the translocation of intestinal bacteria. Antonie van Leeuwenhoek 1990; 58: 87 - 93.
    • 37. Prescot LM, Harley JP, Klein DA. The bacteria: Gram-negative bacteria of general, medical, or industrial importance. In: Microbiology. Englandn: Wm C Brown publisher, 1993: 426 - 49.
    • 38. Zaleznik DF, Kasper DL. Bacteroides species. In: Mandell GL, Douglas RG, Bennett JE. Principles and practice of infectious diseases. Churchill Livingstone ed. New York 1990; 1860 - 1867.
    • 39. Nicas TI, Wu CYE, Hobbs NR Jr, Preston DA, Allen NE. Characterization of vancomycin resistance in Enterococcus faecium and Enterococcus faecalis. Antimicrob Agents Chemother 1989; 33: 1121 - 4.
    • 40. Boyle JM, Soumakis SA, Rendo A, et al. Epidemiologic analysis and genotypic characterization of a nosocomial outbreak of vancomycin-resistant enterococci. J Clin Microbiol 1993; 31 (5): 1280 - 5.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article

Collected from