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Khan, Rahmat Ali; Alkreathy, Huda Mohammad; Saboorshah, Abdus; Ahmed, Mushtaq; Khan, Samiullah (2016)
Publisher: Co-Action Publishing
Journal: Food & Nutrition Research
Languages: English
Types: Article
Subjects: Nutrition. Foods and food supply, Original Article, TX341-641, CCl4, lungs, Trifolium alexandrinum, lipid peroxidation

Classified by OpenAIRE into

mesheuropmc: digestive system
Background: In Pakistan numerous medicinal floras has used in the treatment of various human ailments. Among them Trifolium alexandrinum L. is traditionally used in the curing of disease. Presently we designed to ascertain the protective role of Trifolium alexandrinum methanolic extracts (TAME) against carbon tetrachloride (CCl4)-induced lung injury and oxidative stress in rats.Methods: Exposure to CCl4 induces oxidative stress and causes tissue damage by the induction of CCl4 free radicals. Twenty-four male albino rats were divided equally into four groups. Rats in group I had free access to drinking water and laboratory food. Group II was treated with 1 ml/kg body weight (b.w.) CCl4 (30% in olive oil). Groups III and IV rats were fed (p.o.) 200 mg/kg b.w. TAME and 50 mg/kg b.w. silymarin after 24 h of CCl4 treatment for 2 weeks.Results: Administration of CCl4 caused a significant (p<0.01) decrease in the activities of antioxidant enzymes (catalase, peroxidase, glutathione peroxidase, glutathione-S-transferase), and glutathione contents were decreased; however, thiobarbituric acid-reactive substances were increased (p<0.01). The alterations caused by CCl4 were significantly (p<0.01) reversed toward control levels by supplementation of TAME and silymarin.Conclusion: These results suggest that in rats TAME and silymarin could protect the lungs against CCl4-induced oxidative damage.Keywords: lungs; CCl4; lipid peroxidation; Trifolium alexandrinum(Published: 7 November 2016)Citation: Food & Nutrition Research 2016, 60: 30433 - http://dx.doi.org/10.3402/fnr.v60.30433
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    • 1. Jackson RM. Molecular, pharmacologic, and clinical aspects of oxygen-induced lung injury. Clin Chest Med 1990; 11: 73 86.
    • 2. Fisher AB, Forman HJ, Glass M. Mechanisms of pulmonary oxygen toxicity. Lung 1984; 162: 255 9.
    • 3. Clark JM, Lambertsen CJ. Pulmonary oxygen toxicity: a review. Pharmacol Rev 1971; 23: 37 133.
    • 4. Crystal RG. Oxidants and respiratory tract epithelial injury: pathogenesis and strategies for therapeutic intervention. Am J Med 1991; 91: 39S 44S.
    • 5. Conner EM, Grisham MB. Inflammation, free radicals, and antioxidants. Nutrition 1996; 12: 274 7.
    • 6. Mannino DM, Homa DM, Akinbami LJ, Ford ES, Redd SC. Chronic obstructive pulmonary disease surveillance United States, 1971 2000. MMWR Surveill Summ 2002; 51: 1 16.
    • 7. Cerutti PA. Oxidant stress and carcinogenesis. Eur J Clin Invest 1991; 21: 1 5.
    • 8. Abraham P, Wilfred G, Cathrine SP. Oxidative damage to lipids and proteins of the lungs, testis and kidney of rats during CCl4 intoxication. Clin Acta 1999; 289: 177 9.
    • 9. Daniels WM, Reiter RJ, Melchiorri D, Sewerynek E, Pablos MI, Ortiz GG. Melatonin counter acts lipid peroxidation induced by carbon tetrachloride but does not restore glucose6-phosphatase activity. J Pineal Res 1995; 19: 1 6.
    • 10. Weber LW, Boll M, Stampfl A. Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Crit Rev Toxicol 2003; 33: 105 36.
    • 11. Dogukan A, Akpolat N, Celiker H, Ilhan N, Bahcecioglu IH, Gunal AI. Protective effect of interferon-alpha on carbon tetrachloride-induced nephrotoxicity. J Nephrol 2003; 16: 81 4.
    • 12. Adewole SO, Salako AA, Doherty OW, Naicker T. Effect of melatonin on carbon tetrachloride-induced kidney injury in Wistar rats. Afr J Biomed Res 2007; 10: 153 64.
    • 13. Pradeep K, Mohan CV, Gobianand K, Karthikeyan S. Silymarin modulates the oxidant-antioxidant imbalance during diethylnitrosamine induced oxidative stress in rats. Eur J Pharmacol 2007; 560(2 3): 110 16.
    • 14. Danciu C, Vlaia L, Fetea F, Hancianu M, Coricovac DE, Ciurlea SA, et al. Evaluation of phenolic profile, antioxidant and anticancer potential of two main representants of Zingiberaceae family against B164A5 murine melanoma cells. Biol Res. 2015; 48(1): 1.
    • 15. Antunes LMG, Darin JDC, Bianchi MLP. Effects of the antioxidants curcumin or selenium on cisplatin-induced nephrotoxicity and lipid peroxidation in rats. Pharm Res 2001; 43(2): 145 50.
    • 16. Khar A, Ali AM, Pardhasaradhi BV. Induction of stress response renders human tumor cell lines resistant to curcuminmediated apoptosis: role of reactive oxygen intermediates. Cell Stress Chap 2001; 6(4): 368 76.
    • 17. Khan AV, Ahmed QU, Shukla I, Khan AA. Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases. Asian Pac J Trop Biomed 2012; 3: 189 94.
    • 18. Lowry OH, Rosenberg NJ, Farr AL, Randall RJ. Protein measurement with Folin Phenol reagent. J Biol Chem 1951; 193: 265 75.
    • 19. Chance B, Maehly AC. Assay of catalase and peroxidases. Methods Enzymol 1955; 11: 764 75.
    • 20. Habig WH, Pabst MJ, Jakoby WB. Glutathione-S-transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 249: 7130 9.
    • 21. Mohandas J, Marshal JJ, Duggin GG, Horvath JS, Tiller DJ. Differential distribution of glutathione and glutathione-related enzymes in rabbit kidney. Possible implications in analgesic nephropathy. Biochem Pharmacol 1984; 33: 1801 7.
    • 22. Carlberg I, Mannervik EB. Glutathione level in rat brain. J Biol Chem 1975; 250: 4475 80.
    • 23. Wright JR, Colby HD, Miles PR. Cytosolic factors that affect microsomal lipid peroxidation in lung and liver. Arch Biochem Biophys 1981; 206: 296 304.
    • 24. Venkatesan N, Punithavathi V, Chandrakasan G. Curcumin protects bleomycin-induced lung injury in rats. Life Sci 1997; 61(6): 51 8.
    • 25. Khan RA, Khan MR, Sahreen S. Protective effect of Sonchus asper extracts against experimentally-induced lung injuries in rats: a novel study. Exp Toxicol Pathol 2012; 64: 725 31. doi: http://dx.doi.org/10.1016/j.etp.2011.01.007
    • 26. Singh N, Kamath V, Narasimhamurthy K, Rajini PS. Protective effects of potato peel extract against carbon tetrachlorideinduced liver injury in rats. Environ Toxicol Pharmacol 2008; 26: 242 6.
    • 27. Ohkawa H, Ohishi N, Yogi K. Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351 8.
    • 28. Vardavas CI, Majchrzak D, Wagner K-H, Elmadfa I, Kafatos A. The antioxidant and phylloquinone content of wildly grown greens in Crete. Food Chem 2006; 99: 813 21.
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