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Beighton, D.; Smith, K.; Glenister, D. A.; Salamon, K.; Keevil, C. W. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
Complex communities of dental plaque bacteria were grown under a variety of continuous culture conditions including one in which hog gastric mucin constituted the principal carbon and nitrogen sources. Washed cells and culture supernatants were assayed for fifteen exoglycosidase activities and for the ability to hydrolyse eleven synthetic peptidase substrates. Under all conditions, cultures elaborated a wide range of exoglycosidases and several peptidase activities that were principally cell-associated. Highest exoglycosidase activities were obtained with cultures growing under conditions in which hog gastric mucin constituted the principal carbon and nitrogen sources. Under these conditions, the principal species isolated were Bacteroides spp. and there was extensive utilisation of protein and carbohydrate moieties of the hog gastric rnucin. These data support the hypothesis that supra-gingival plaque bacteria are able in vivo to obtain their nutritional requirements from salivary glycoproteins.Keywords: Dental plaque; Chemostat; Glycosidases; Proteases; Mucin.
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    • 1. Abiko Y , Hayakawa M, Murai S, Takiguchi H. (1985).Glycylprolyl dipeptidylaminopeptidasefrom Bacteroides gingivalis. Journal of Dental Research 64,106-1 11.
    • 2 . Allen A. (1978). Structure of gasterointestinal mucus glycoproteins and the viscous and gel-forming properties of mucus. British Medical Bulletin 34, 28-33.
    • 3. Beckers HJA, Van Der Hoeven JS. (1982). Growth rates of Actinomyces viscosus and Streptococcus mutans during early colonization of tooth surfaces in gnotobiotic rats. Infection and Immunity 35, 583-587.
    • 4. Beighton D, Hayday H. (1986). The influence of the availability of dietary food on the growth of streptococcion the molar teeth of monkeys (Maraca fascicularis). Archives of Oral Biology 31,449-454.
    • 5. Beighton D, Smith K. (1986). The modulation of exoglycosidic enzymes in the supragingival plaque of macaque monkeys. FEMS Microbiology Letters 34,319-322.
    • 6. Beighton D, Smith K, Hayday H. (1986). The growth of bacteria and the production of exoglycosidic enzymes in the dental plaque of macaque monkeys. Archives of Oral Biology 31,829-835.
    • 7. Boas NF. (1953). Method for the determination of hexosamines in tissues. Journal of Biological Chemistry 204,553-563.
    • 8. Bowden GH, Hardie JM. (1971). Anaerobic organismsfrom the human mouth. In: ShaptonDA, Board RG (Eds),Isolation of Anaerobes. Society for Applied Bacteriological Techniques Series No. 5. Academic Press, London. 177-205.
    • 9. Bradshaw DJ, Dowsett AB, Keevil CW. (1986). A chemostat model for formation of bacterial films on acrylic tiles. In: Borriello SP, Hardie JM (Eds), Recent Advances in Anaerobic Bacteriology. Martinus Nijhoff Publishers, Dordrecht, The Netherlands.327-329.
    • 10. Cowman RA, Fitzgerald RJ. (1975). Effects of oral streptococcion electrophoreticproperties of human salivaryanionicproteins.JournafofDental Research 54,298-303.
    • 11. Dische Z, Shettles LB. (1948). A specific color method for methylpentosesand their determination. Journal of Biological Chemistry 175,595403.
    • 12. Fukasawa K, Hiraoka BY, Fukasawa KM, Harada M. (1 982). Arylamidase activities specific for proline, tyrosine and basic amino acid residues in some oral bacteria. Journal of Dental Research 61, 818-820.
    • 13. Fukasawa KM, Harada M. (1981) Purificationand properties of dipeptidyl peptidase IV from Streptococcus mitis ATCC 9811. Archives of Biochemistry and Biophysics 214 230-237.
    • 14. Glenster DA, Salamon KE, Smith K, Beighton D, Keevil CW. (1988). Enhanced growth of complex communities of dental plaque bacteria in mucinlimited continuous culture. Microbial Ecology in Health and Disease 1,31-38.
    • 15. Herp A, Wu AM, Moschera J. (1979). Current concepts of the structure and nature of mammalian salivarymucous glycoproteins.Molecular and Cellular Biochemistry 23,27-44.
    • 16. Hoskins LC, Boulding ET. (1976). Degradation of blood group antigens in human colon ecosystems. I. In vitro production of ABH blood groupdegrading enzymes by enteric bacteria. Journal of Clinical Investigations 57,63-73.
    • 17. de Jong MH, Van Der Hoeven JS. (1985). Growth of plaque micro-organisms on saliva. Caries Research 20,161, (Abstract 37).
    • 18. Keevil CW, Bradshaw DJ, Dowsett AB, Seary TW. (1987a). Microbial film formation: dental plaque deposition on acrylic tiles using continuous culture techniques. Journal of Applied Bacteriology 62, 129-138.
    • 19. Keevil CW, Dowsett AB, Salamon K , Glennister DA. (1987b). Effectofmucin on plaque formation in the continuous culture biofilm model. Journal of Dental Research (in press).
    • 20. Leach SA. (1963). Release and breakdown of sialic acid from human salivary mucin and its role in the formation of dental plaque. Nature 199,486-487.
    • 21. Leach SA, Hayes M. (1967). Isolation in pure culture of human oral organisms capable of producing neuraminidase. Nature 216,599400.
    • 22. Miller RS, Hoskins LC. (1981). Mucin degradation in the human colon. Fecal population densities of mucin-degrading bacteria estimated by a “most probable number method”. Gastroenterology 81, 759-765.
    • 23. Mokrasch LC. (1954).Analysis of sugar phosphates and sugar mixtures with the anthrone reagent. Journal ofBiologica1 Chemistry 208,55-59.
    • 24. Ohia K, Makinen KK, Loesche WJ. (1986). Purification and characterization of an enzyme produced by Treponema denticola capable of hydrolyzing synthetic trypsin substrates. Infection and Immunity 53,213-220.
    • 25. Oya H, Nagatsu T, Kobayashi Y, Takei M. (1971). Arylaminopeptidase activities in human cariogenic and non-cariogenic oral bacteria. Archives of Oral Biology 16,675-680.
    • 26. Riley PS, Behal FJ. (1971). Amino acid b-Naphthylamide hydrolysis by Pseudomonas aeruginosa arylamidase. Journal of Bacteriology 108, 809-8 16.
    • 27. Sandholm L. (1985). Proteases and their inhibitors in chronic inflammatory periodontal disease. Journal of Clinical Periodontology 13, 19-26.
    • 28. Shah HN, Williams RAD, Bowden GH, Hardie JM. (1976).Comparison of the biochemical properties of Bacteroides melaninogenicus from human dental plaque and other sites. Journal of Applied Bacteriology 41,473-492.
    • 29. Sharon N, Lis H. (1982). Glycoproteins. In: Neurath H, Hill RL. (Eds), The Proteins, Vol. 5 . Academic Press, London. 1-144.
    • 30. Shellis RP. (1978). A synthetic saliva for cultural studies ofdental plaque. Archives of Oral Biology 23, 485-489.
    • 31. Slots J. (1981). Enzymatic characterization of some oral and nonoral gram-negative bacteria with the API ZYM system. Journal of Clinical Microbiology 14,288-294.
    • 32. Smith K, Beighton D. (1986). The effects of the availability of diet on the levels of exoglycosidasesin the supragingival plaque of macaque monkeys. Journal of Dental Research 65, 1349-1 352.
    • 33. Smith K, Beighton D. (1987). Proteolytic activities in the supragingival plaque of macaque monkeys. Archives of Oral Biology 32,473-476.
    • 34. Socransky SS, Manganiello AD, Propos D, Oram V, van Houte, J. (1977). Bacteriological studies of developing supragingival dental plaque. Journal of Periodontal Research 12,9&106.
    • 35. Takahashi T, Asari K, Sat0 N, Yamaya S-I, Sugahara T. (1985). Purification and properties of an aminopeptidase from Treponema phagedenis (Reiter strain). Current Microbiology 12,283-288.
    • 36. Warren L. (1963).Thiobarbituricacid assay of sialic acids. Methods in Enzymology 6,463-465.
    • 37. Wilson RS, Woods A, Ashley SP. (1984). Effect of storage in liquid nitrogen on the recovery of human dental plaque bacteria. Archives of Oral Biology 29, 941-944.
    • 38. Yoshimura F, Nishikaia M, Suzuki T, Hoover, CI, Newbrun E. (1984). Characterization of a trypsinlike protease from the bacterium. Bacteroides gingivalis isolated from human dental plaque. Archives of Oral Biology 29,559-564.
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