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Tuohy, Kieran M.; Ziemer, Cherie J.; Klinder, Annett; Knöbel, Yvonne; Pool-Zobel, Beatrice L.; Gibson, Glenn R. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
The prebiotic effects of lactulose were monitored in a human feeding study. Prebiotics are dietary carbohydrates that have a selective microbial metabolism in the gut, directed towards bacteria seen as beneficial, examples being bifidobacteria and/or lactobacilli. The study was conducted in a double blind, placebo controlled manner. A dose of 10 g per day, half the pharmacological dose, was fed to 10 healthy adult volunteers. In parallel, 10 persons were fed a placebo (glucose/lactose). Both culture based methodologies and genetic probing, based around fluorescent in situ hybridization were used to determine bacterial populations. Faecal water genotoxicity was assessed using the Comet assay to investigate the ability of lactulose to protect against DNA damage. Bifidobacteria showed a statistically significant increase during lactulose intake, whilst genetic probing showed a concomitant decrease in clostridia. Viable plate counts of lactobacilli increased when lactulose was fed, but this was not replicated by the genetic probing. During the trial, none of the recruits experienced any significant adverse gastrointestinal symptoms. Single-cell gel electrophoresis, used to assess faecal water genotoxicity, did not reveal that lactulose intervention reduced genotoxicity. The prebiotic nature of 10 g/day lactulose towards the human gut microbiota has been clearly demonstrated in this study. Lactulose is shown to be an effective food-grade prebiotic for healthy adults particularly in sections of the community with low bi? dobacterial populations. The value of the more direct, culture independent FISH technique in the microbial ecology of the gut has been demonstrated in this study.Keywords: gut microbiota, prebiotic, lactulose, gene probes, faecal water biomarker.
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    • 1. Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 1995; 125: 1401 - 12.
    • 2. Macfarlane GT, Gibson GR. Carbohydrate fermentation, energy transduction and gas metabolism in the human large intestine. In: Mackie RI, White BH, eds. Gastrointestinal microbiology. London: Chapman & Hall, 1997: 269 - 318.
    • 3. Gibson GR, Wang X. Regulatory effects of bi dobacteria on the growth of other colonic bacteria. J Appl Bacteriol 1994; 77: 412 - 20.
    • 4. Hudault S, Lie´vin V, Bernet-Camard M-F, Servin AL. Antogonistic activity exerted in vitro and in vivo by Lactobacillus casei (Strain GG) against Salmonella typhimurium C5 infection. Appl Environ Microbiol 1997; 63: 513 - 8.
    • 5. Jacobsen CN, Rosenfeldt Nielsen V, Hayford AE, Møller PL, Michaelsen KF, Paerregaard A, Sandstro¨ m B, Tvede M, Jakobsen M. Screening of probiotic activities of forty-seven strains of Lactobacillus spp. By in vitro techniques and evaluation of the colonization ability of ve selected strains in humans. Appl Environ Microbiol 1999; 65: 4949 - 56.
    • 6. Lie´vin V, Peiffer I, Hudault S, Rochat F, Brassart D, Neeser J-R, Servin AL. Bi dobacterium strains from resident infant human gastrointestinal microbiota exert antimicrobial activity. Gut 2000; 47: 646 - 52.
    • 7. McCracken VJ, Gaskins HR. Probiotics and the immune system. In: Tannock GW, ed. Probiotics a critical review. Wymondham: Horizon Scienti c Press, 1999: 85 - 111.
    • 8. Tuomola EM, Ouwehand AC, Salminen SJ. The effect of probiotic bacteria on the adhesion of pathogens to human intestinal mucus. FEMS Immunol Med Microbiol 1999; 26: 137 - 42.
    • 9. Forestier C, De Champs C, Vatoux C, Joly B. Probiotic activities of Lactobacillus casei rhamnosus : in vitro adherence to intestinal cells and antimicrobial properties. Res Microbiol 2001; 152: 167 - 73.
    • 10. Conway PL. Microbial ecology of the human large intestine. In: Gibson GR, Macfarlane GT, eds. Human colonic bacteria: role in nutrition, physiology and pathology. Boca Raton: CRC Press, 1995: 1 - 24.
    • 11. Roberfroid MB, Bornet F, Bouley C, Cummings JH. Colonic microbiota: Nutrition and Health Nutr Revs 1995; 53: 127 - 30.
    • 12. Tannock GW. A fresh look at the intestinal microbiota. In: Tannock GW, ed. Probiotics a critical review. Wymondham: Horizon Scienti c Press, 1999: 5 - 14.
    • 13. Steer T, Carpenter H, Tuohy K, Gibson GR. Perspectives on the role of the human gut microbiota in health and disease and its modulation by pro- and prebiotics. Nutr Res Revs 2000; 13: 229 -54.
    • 14. Gibson GR, Berry-Ottaway P, Rastall RA. Prebiotics: new developments in functional foods. Oxford, UK: Chandos Publishing Limited, 2000.
    • 15. Salminen S, Salminen E. Lactulose, lactic acid bacteria, intestinal microecology and mucosal protection. Scand J Gastroenterol 1997; 32 (Suppl 222): 45 - 8.
    • 16. Clausen MR, Mortensen PB. Lactulose, disaccharides and colonic ora. Drugs 53; 930-942.
    • 17. Cummings JH, Bingham SA, Heaton KW, Eastwood MA. Fecal weight, colon cancer risk, and dietary intake of nonstarch polysaccharides (dietary ber). Gastroenterol 1992; 103: 1783 - 9.
    • 18. Terada A, Hara H, Kataoka M, Mitsuoka T. Effect of lactulose on the composition and metabolic activity of the human faecal microbiota. Microb Ecol Health Dis 1992; 5: 43 - 50.
    • 19. Ballongue J, Schumann C, Quignon P. Effects of lactulose and lactitol on colonic microbiota and enzymatic activity. Scand J Gastroenterol 1997; 32: 41 - 4.
    • 20. Hirayama K, Rafter J. The role of lactic acid bacteria in colon cancer prevention: mechanistic considerations. Ant Van Leeuwenhoek 1999; 76: 391 - 4.
    • 21. Bolognani F, Rumney CJ, Rowland IR. In uence of carcinogen binding by lactic acid-producing bacteria on tissue distribution and in vivo mutagenicity of dietary carcinogens. Food Chem Toxicol 1997; 35: 535 -45.
    • 22. Langendijk PS, Schut F, Jansen GJ, Raangs GW, Kamphuis GR, Wilkinson MHF, Welling GW. Quantitative uorescent in situ hybridization of Bi dobacterium spp. with genus-speci c 16S rRNA-targeted probes and its application in faecal samples. Appl Environ Microbiol 1995; 61: 3069 - 75.
    • 23. Hartemink R, Rombouts FM. Comparison of media for the detection of bi dobacteria, lactobacilli and total anaerobes from faecal samples. J Microbiol Methods 1999; 36: 181 - 92.
    • 24. Wilson KH, Blitchington RB. Human colonic bacteria studied by ribosomal DNA sequence analysis. Appl Environ Microbiol 1996; 62: 2273 - 8.
    • 25. Suau A, Bonnet R, Sutren M, Godon J-J, Gibson GR, Collins MD, Dore´ J. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 1999; 65: 4799 - 807.
    • 26. Beerens H. An elective and selective isolation medium for Bi dobacterium spp. Letts. Appl. Microbiol. 1990; 11: 155 - 7.
    • 27. Silvi S, Rumney CJ, Rowland IR. An assessment of three selective media for bi dobacteria in faeces. J Appl Bacteriol 1996; 81: 561 - 4.
    • 28. Hartemink R, Kok BJ, Weenk GH, Rombouts FM. Raf nose-Bi dobacterium (RB) agar, a new selective medium for bi dobacteria. J Microbiol Methods 1996; 27: 33 - 43.
    • 29. Manz W, Amann R, Ludwig W, Vancanneyt M, Schleifer K-H. Application of a suite of 16S rRNA-speci c oligonucleotide probes designed to investigate bacteria of the phylum cytophaga- avobacter-bacteroides in the natural environment. Microbiol 1996; 142: 1097 - 106.
    • 30. Franks AH, Harmsen HJM, Raangs GC, Jansen GJ, Schut F, Welling GW. Variations of bacterial populations in human faeces measured by uorescent in situ hybridization with group-speci c 16S rRNA-targeted oligonucleotide probes. Appl Environ Microbiol 1998; 64: 3336 - 45.
    • 31. Harmsen HJM, Elfferich P, Schut F, Welling GW. A 16S rRNA-targeted probe for detection of lactobacilli and enterococci in faecal samples by uorescent in situ hybridization. Microb Ecol Health Dis 1999a; 11: 3 - 12.
    • 32. Porter KG, Feig YS. The use of DAPI for identifying and counting aquatic microbiota. Limnol Oceanog 1980; 25: 943 - 8.
    • 33. Harmsen HJM, Gibson GR, Elfferich P, Raangs GC, Wideboer-Veloo ACM, Argaiz A, Roberfroid MB, Welling GW. Comparison of viable cell counts and uorescent in situ hybridization using speci c rRNA-based probes for the quanti cation of human faecal bacteria. FEMS Microb Ecol 1999b ; 183: 125 - 9.
    • 34. Tuohy KM, Finlay RK, Wynne AG, Gibson GR. A human volunteer study on the prebiotic effects of HP-inulin - faecal bacteria enumerated using uorescent in situ hybridization (FISH). Anaerobe 2001a; 7: 113 - 8.
    • 35. Osswald K, Becker TW, Grimm M, Jahreis G, Pool-Zobel BL. Inter- and intra-individual variation of faecal watergenotoxicity in human colon cells. Mutat Res 2000; 472: 59 - 70.
    • 36. Augeron C, Laboisse CL. Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. Cancer Res 1984; 44: 3961 - 9.
    • 37. Pool-Zobel BL, Abrahamse SL, Collins AR, Kark W, Gugler R, Oberreuther D, Siegel EG, Treptow-van Lishaut S, Rechkemmer G. Analysis of DNA strand breaks, oxidized bases and glutathione S-transferase P1 in human colon cells. Cancer Epidemiol Biomark Prev 1999; 8: 609 - 14.
    • 38. Rowland IR. Toxicology of the colon: role of the intestinal microbiota. In: Gibson GR, Macfarlane GT, eds. Human colonic bacteria: role in nutrition, physiology and pathology. Boca Raton: CRC Press, 1995: 155 - 74.
    • 39. Pool-Zobel BL, Neudecker C, Domizlaff I, Ji S, Schillinger U, Rumney C, Moretti M, Vilarini I, Scassellati-Sforzolini R, Rowland I. Lactobacillus - and Bi dobacterium -mediated antigenotoxicity in the colon of rats. Nutr Cancer 1996; 26: 365 - 80.
    • 40. Tuohy KM, Kolida S, Lustenberger AM, Gibson GR. The prebiotic effects of biscuits containing partially hydrolyzed guar gum and fructo-oligosaccharides - a human volunteer study. Brit J Nutr 2001b ; 86: 341 - 8.
    • 41. Roberfroid MB, Van Loo JAE, Gibson GR. The bi dogenic nature of chicory inulin and its hydrolysis products. J Nutr 1998; 128: 11 -9.
    • 42. Harmsen HJM, Raangs GC, He T, Degener JE, Welling GW. Extensive set of 16S rRNA-based probes for detection of bacteria in human feces. Appl Environ Microbiol 2002; 68: 2982 - 90.
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