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Sissons, C. H.; Wong, L.; Cutress, T. W. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
Subjects:
The regulation of plaque urease activity by urea, ammonia and arginine was examined using microcosm dental plaques cultured in a multi-plaque ‘artificial mouth‘ from the mixed oral microbiota. The plaques were supplied periodically with sucrose and continuously (3.6 ml/h/plaque) with a much containing medium (BMM) supplemented either with urea at concentrations found in saliva, or with arginine, or ammonia. Urease levels in plaque range from 400 to 1500 nmol NH3/min/mg plaque protein. In the absence of urea supplementation, microcosm plaque urease levels were usually less than 100 nmol NH3/min/mg protein. They decreased after 2 to 3 wks growth and were not restored by subsequent urea application. BMM containing 1, 5 and 20 mmol/l urea throughout growth produced a proportional increase in urease levels initially, similar to those observed in plaques in vivo. Subsequently, urease levels decreased as the plaques aged. BMM supplemented with arginine (1 mmol/l) or ammonia (2 mmoU1) reduced urease levels. The high urease levels found in supragingival plaque can be explained by the availability of urea at concentrations secreted in saliva. Ammonia and arginine lowered plaque urease levels.Keywords: microcosm plaque; plaque urease; urea; ammonia; arginine.
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