Remember Me
Or use your Academic/Social account:


Or use your Academic/Social account:


You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.


Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message


Verify Password:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Isoshima, O.; Ohta, H.; Kurikara, H.; Kato, K.; Fukui, K.; Murayama, Y. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article

Classified by OpenAIRE into

mesheuropmc: stomatognathic diseases, stomatognathic system
The distribution of Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella denticola, Prevotella intermedia, Prevotella loescheii, and Prevotella melaninogenica in periodontally healthy and diseased sites of patients was investigated. The periodontal pockets of two moderate periodontitis patients were scored clinically and sampled microbiologically at their first visit to Okayama University Dental Hospital. A total of 427 isolates of black-pigmented, anaerobic gram-negative rods (BPARs) were identified using a quick method of DNA-DNA hybridisation. In both subjects, all six BPAR species examined were distributed in clusters in certain areas of the dentition. P. gingivalis and Pr. intermedia were the most prevalent species identified. The DNA fingerprinting analysis of P. gingivalis isolates demonstrated that such a cluster was composed of one or two clonal types of the same species, and that five clonal types of the species were present in the whole dentition. For one subject, clinical and microbiological examinations were performed again 3 months later. P. gingivalis and Pr. intermedia were still prevalent and distributed in clusters. Among the five preexisting clonal types of P. gingivalis, four were still found, and six other types were recognised. Site-to-site and time-to-time variations in the species profile of BPAR and the clonal profile of P. gingivalis in individual sites may explain the uneven progression of disease in the dentition.Keywords: periodontal microflora, periodontitis, Porphyromonas gingivalis, Prevotella intermedia, DNA fingerprinting
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1 . Barua PK, Dyer DW, Neiders ME. (1990). Effect of iron limitation on Bacteroides gingivalis. Oral Microbiology and Immunology 5, 263-268.
    • 2. Bramanti TE, Holt SC. (1991). Roles of porphyrins and host iron transport proteins in regulation of growth of Porphyromonas gingivalis W50. Journal of Bacteriology 173, 7330-7339.
    • 3. Carman RJ, Ramakrishnan MD, Harper FH. (1990). Hemin levels in culture medium of Porphyromonas (Bacteroides) gingivalis regulate both hemin binding and trypsinlike protease production. Infection and Immunity 58, 4016-40 19.
    • 4. Chen C-K, Sunday GJ, Zambon JJ, Wilson ME. (1 990). Restriction endonuclease analysis of Eikenella corrodens. Journal of Clinical Microbiology 28, 1265-1270.
    • 5. Ezaki T, Hashimoto Y, Takeuchi N, Yamamoto H, Liu S, Miura H, Matsui K, Yabuuchi E. (1988). Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. Journal of Clinical Microbiology 26, 1708-1713.
    • 6. Ezaki T, Takeuchi N, Liu S, Kai A, Yamamoto H, Yabuuchi E. (1988). Small-scale DNA preparation for rapid genetic identification of Campylobacter the growth and virulence of P. g i n g i v a l i ~ . ~ , ~ ~ ~sp~e,c~ies~ without radioisotope. Microbiology and Moreover, Shah and Gharbia41 and Kay et a1.I4 have demonstrated that some P. gingivalis strains produce haemolysins which might function in the release of haem from erythrocytes. Thus, in the case of P. gingivalis, the ability to produce haemolysins may confer a selective advantage over other haemrequiring bacteria and account for the observed predominance of this species in bleeding pockets. It has been noted by several investigators that there is considerable variability in the virulence of different P. gingivalis strains when they are injected into mice.9.30,4I9f this heterogeneity is also true for the P.gingivalis strains harboured in subject 1, the irregular pattern of active periodontal destruction in that subject may be explained by the distribution of such virulent strains. Further studies on the ability of different clonal types to produce abscesses in the mouse model are currently in progress in our laboratory. Immunology 32, 141-150.
    • 7. Ezaki T, Hashimoto Y, Yabuuchi E. (1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. International Journal of Systematic Bacteriology 39, 224-229.
    • 8. Grenier D, Mayrand D. (1986). Nutritional relationships between oral bacteria. Infection and Immunity 53, 6 16-620.
    • 9. Grenier D, Mayrand D. (1987). Selected characteristics of pathogenic and nonpathogenic strains of Bacteroides gingivalis. Journal of Clinical Microbiology 25, 738-740.
    • 10. Hamilton IR, McKee AS, Bowden GH. (1989). Growth and metabolic properties of Bacteroides intermedius in anaerobic continuous culture. Oral Microbiology and Immunology 4, 89-97.
    • 1 1 . Holdeman LV, Cat0 EP, Moore WEC. (1977). Anaerobe Laboratory Manual., 4th edn. Anaerobe Laboratory, Virginia Polytechnic Institute and State University, Blacksburg, Virginia.
    • 12. Johnson JL, Holdeman LV. (1983). Bacteroides intermedius comb. nov. and descriptions of Bacteroides corporis sp. nov. and Bacteroides levii sp. nov. International Journal of Systematic Bacteriology 33, 15-25.
    • 13. Johnson JL. (1984). Nucleic acids in bacterial classification. In: Krieg NR, Holt JG (eds) Bergey's Manual of Systematic Bacteriology. Williams and Wilkins, Baltimore, vol. 1, pp. 8-11.
    • 14. Kay HM, Birss AJ, Smalley JW.(1990). Haemagglutinating and haemolytic activity of the extracellular vesicles of Bacteroides gingivalis W50. Oral Microbiology and Immunology 5, 269-274.
    • 15. Kornman KS, Loesche WJ. (1982). Effects of estradiol and progesterone on Bacteroides rnelaninogenicus and Bacteroides gingivalis. Infection and Immunity 35, 256-263.
    • 16. Loesche WJ, Syed SA. (1978). Bacteriology of human experimental gingivitis 11. Effect of plaque and gingivitis score. Infection and Immunity 21, 830-839.
    • 17. Loesche WJ, Gusberti F, Mettraux G, Higgins T, Syed S. (1983). Relationships between oxygen tension and subgingival flora in untreated human periodontal pockets. Infection and Immunity 42, 659-667.
    • 18. Loesche WJ. (1988). Ecology of the oral flora. In: Newman MG, Nisengard R (eds) Oral Microbiology and Immunology. WB Saunders Company, Philadelphia, pp. 351-366.
    • 19. Loos BG, Mayrand D, Genco RJ, Dickinson DP. (1990). Genetic heterogeneity of Porphyromonas (Bacteroides) gingivalis by genomic DNA fingerprinting. Journal of Dental Research 69, 1488- 1493.
    • 20. Loos BG, Van Winkelhoff AJ, Dunford RG, Genco RJ, De Graaff J, Dickinson DP, Dyer DW. (1992). A statistical approach to the ecology of Porphyromonas gingivalis. Journal of Dental Research 71, 353-358.
    • 21. Mayrand D, Holt SC. (1988). Biology of asaccharolytic black-pigmented Bacteroides species. Microbiological Reviews 52, 1 3 4 152.
    • 22. McDermid AS, McKee AS, Ellwood DC, Marsh PD. (1986). The effect of lowering the pH on the composition and metabolism of a community of nine oral bacteria grown in a chemostat. Journal of General Microbiology 132, 1205-1214.
    • 23. McDermid AS, McKee AS, Marsh PD. (1988). Effect of environmental p H on enzyme activity and growth of Bacteroides gingivalis W50. Infection and Immunity 56, 109&1100.
    • 24. McKee AS, McDermid AS, Baskerville A, Dowsett AB, Ellwood DC, Marsh PD. (1986).Effect of hemin on the physiology and virulence of Bacteroides gingivalis W50. Infection and Immunity 52, 349% 355.
    • 25. Meinkoth J, Wahl G. (1984). Hybridization of nucleic acids immobilized on solid supports. Analytical Chemistry 138, 267-284.
    • 26. Minhas T, Greenman J, Schaffer AG. (1991). Effects of mucin, haemoglobin and collagen on the maximum specific growth rate, biomass and hydrolytic enzyme production of Porphyromonas gingivalis in continuous culture. Microbial Ecology in Health and Disease 4, 31 1-318.
    • 27. Mombelli A, McNabb H, Lang NP. (1991). Blackpigmenting Gram-negative bacteria in periodontal disease. I. Topographic distribution in the human dentition. Journal of Periodontal Research 26, 301- 307.
    • 28. Moore WEC, Holdeman LV, Cat0 EP, Good IJ, Smith EP, Ranney RR, Palcanis KG. (1984). Variation in periodontal floras. Infection and Immunity 46, 720-726.
    • 29. Murayama Y, Isoshima 0, Kurimoto K, Kobayashi Y, Kurihara H, Nomura Y, Ohta H, Kato K. (1991). Antibiotic therapy in crevicular regions for treatment of periodontal disease. In: Hamada S, Holt SC, McGhee JR (eds) Periodontal Disease: Pathogens and Host Immune Responses. Quintessence, Tokyo, pp. 393405.
    • 30. Neiders ME, Chen PB, Suido H, Reynolds HS, Zambon JJ, Shlossman M, Genco RJ. (1989). Heterogeneity of virulence among strains of Bacteroides gingivalis. Journal of Periodontal Research 24, 192-1 98.
    • 31. Nisimura F, Nagai A, Kurimoto K, Isoshima 0, Takashiba S, Kobayashi M, Akutsu I, Kurihara H, Nomura Y, Murayama Y, Ohta H, Kato K. (1990). A family study of a mother and daughter with increased susceptibility to early-onset periodontitis: Microbiological, immunological, host defensive, and genetic analyses. Journal of Peuiodontology 61, 755-765.
    • 32. Ohta H, Kokeguchi S, Fukui K, Kato K. (1986). Actinobacillus (Haemophilus) actinomycetemcomitans in periodontal disease. Microbiology and Immunology 30, 629-643.
    • 33. Ohta H, Gottschal JC, Fukui K, Kato K. (1990). Interrelationships between Wolinella recta and Streptococcus sanguis in mixed continuous cultures. Microbial Ecology in Health and Disease 3, 237- 244.
    • 34. Page RC, Schroeder HE. (1982). Periodontitis in Man and Other Animals: A Comparative Review. Karger, Basel, New York.
    • 35. Rawlinson A, Duerden BI, Goodwin L. (1991). Microbial flora of bleeding and non-bleeding pockets of variable depth in adult periodontitis. Microbial Ecology in Health and Disease 4, 383-389.
    • 36. Roberts MC, Moncla B, Kenny GE. (1987). Chromosomal DNA probes for the identification of Bacteroides species. Journal of General Microbiology 133, 1423-1430.
    • 37. Ronald MA, Lawrence CP. (1985). Handbook of Microbiological Media. CRC Press, Boca Raton, Florida, pp. 203-204.
    • 38. Saito H, Miura K. (1963). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochemica et Biophysica Acta 72, 619- 629.
    • 39. Schei 0, Waerhaug J, Lovdal A, Arno A. (1959). Alveolar bone loss as related to oral hygiene and age. Journal of Periodontology 30, 7-15.
    • 40. Shah HN, Collins DM. (1988). Proposal for reclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis, and Bacteroides endodontalis in a new genus, Porphyromonas. International Journal of Systematic Bacteriology 38, 128-1 31.
    • 41. Shah HN, Gharbia SE. (1989). Lysis of erythrocytes by the secreted cysteine proteinase of Porphyromonas gingivalis W83. FEMS Microbiology Letters 61, 213-218.
    • 42. Shah HN, Collins DM. (1990). Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides. International Journal of Systematic Bacteriology 40, 205-208.
    • 43. Silness J, Loe H. (1964). Periodontal disease in pregnancy. 11. Correlation between oral hygiene and periodontal condition. Acta Odontologica Scandinavica 22, 121-135.
    • 44. Singh S, Cianciola L, Genco RJ. (1977). The suppurative index: an indicator of active periodontal disease. Journal of Dental Research 56 (Special Issue B), B200 (AADR Abstract 593).
    • 45. Slots J. (1982). Importance of black-pigmented Bacteroides in human periodontal disease. In: Genco RJ, Mergenhagen SE (eds) Host-Parasite Interactions in Periodontal Diseases. American Society for Microbiology, Washington, pp. 27-45.
    • 46. Slots J, Listgarten MA. (1988). Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases. Journal of Clinical Periodontology 15, 85-93.
    • 47. Smalley JW, Birss AJ, McKee AS, Marsh PD. (1991). Haemin-restriction influences haeminbinding, haemagglutination and protease activity of cells and extracellular membrane vesicles of Porphyromonas gingivalis W50. FEMS Microbiology Letters 90, 63-68.
    • 48. Ter Steeg PF, Van der Hoeven JS, De Jong MH, Van Munster PJJ, Jansen MJH. (1988). Modelling the gingival pocket by enrichment of subgingival microflora in human serum in chemostats. Microbial Ecology in Health and Disease 1, 73-84.
    • 49. Van Steenbergen TJM, Delemarre FGA, Namavar F, De Graaff J. (1987). Differences in virulence within the species Bacteroides gingivalis. Antonie van Leeuwenhoek 53, 233-244.
    • 50. Van Steenbergen TJM, Van Winkelhoff AJ, De Graaff J. (1991). Black-pigmented oral anaerobic rods: Classification and role in periodontal disease. In: Hamada S, Holt SC, McGhee JR (eds) Periodontal Disease: Pathogens and Host Immune Responses. Quintessence, Tokyo, pp. 41-52.
    • 51. Van Winkelhoff AJ, Van Steenbergen TJM, De Graaff J. (1988). The role of black-pigmented Bacteroides in human oral infections. Journal of Clinical Periodontology 15, 145-1 55.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article

Collected from