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Goulhen, Florence; Grenier, Daniel; Mayrand, Denis (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
Actinobacillus actinomycetemcomitans is a periodontopathogenic bacterium associated with active lesions of localized aggressive periodontitis. Cells of A. actinomycetemcomitans grown to mid-log phase, diluted or undiluted, and subjected to a sublethal heat stress demonstrated a partial protection to a subsequent lethal heat stress. On the contrary, stationary phase heat-stressed cells did not exhibit this transient thermal protection. Heat-stressed cells at sublethal temperature showed a higher expression of GroEL and DnaK proteins by immunoblotting, which was in agreement with de novo synthesis. A. actinomycetemcomitans was found to be more sensitive to a pH than to a heat stress. A transient protection to a lethal acid stress was demonstrated when cells were previously subjected to a sublethal acid stress. On the contrary, such transient protection did not occur for alkaline stresses. DnaK but not GroEL proteins made up a high proportion of the total protein synthesized following acid stress. The protection phenomenon is highly complex and heat shock proteins, which may help A. actinomycetemcomitans to support subsequent lethal conditions, are not expressed in the same way during the different stresses investigated.Key words: Actinobacillus actinomycetemcomitans, periodontitis, heat shock protein, stress tolerance.
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    • 1. Meyer DH, Fives-Taylor PM. The role of Actinobacillus actinomycetemcomitans in the pathogenesis of periodontal disease. Trends Microbiol 1997; 5: 224 /8.
    • 2. Fives-Taylor PM, Meyer DH, Mintz KH, Brisette C. Virulence factors of Actinobacillus actinomycetemcomitans. Periodontol 2000(1999); 20: 136 /67.
    • 3. Bowden GHW, Hamilton IR. Survival of oral bacteria. Crit Rev Oral Biol 1998; 9: 54 /85.
    • 4. Ellis RJ. Stress proteins as molecular chaperones. In: Van Eden W, Young DB, eds. Stress Proteins in Medicine. New York: Marcel Decker, 1996: 1 /26.
    • 5. Flynn GC, Campbell TG, Rothman JE. Peptide binding and release by proteins implicated as catalysts of protein assembly. Science 1989; 245: 385 /90.
    • 6. Scorpio A, Johnson P, Laquerre A, Nelson DR. Subcellular localization and chaperone activities of Borrelia burgdorferi HSP60 and HSP70. J Bacteriol 1994; 176: 6449 /56.
    • 7. Tilly K, Georgopoulos C. Evidence that the two Escherichia coli groE morphogenetic gene products interact in vivo. J Bacteriol 1982; 149: 1082 /8.
    • 8. Van Rosmalen M, Saier MH. Structural and evolutionary relationships between two families of bacterial extracytoplasmic chaperone proteins which function cooperatively in fimbrial assembly. Res Microbiol 1993; 144: 507 /27.
    • 9. Koga T, Kusuzaki T, Asakawa H, Senpuku H, Nishihara T, Noguchi T. The 64-kilodalton GroEL-like protein of Actinobacillus actinomycetemcomitans. J Periodont Res 1993; 28: 475 /7.
    • 10. Løkensgard I, Bakken V, Schenck K. Heat shock response in Actinobacillus actinomycetemcomitans. FEMS Immunol Med Microbiol 1994; 8: 321 /8.
    • 11. Nakano Y, Inai Y, Yamashita Y, Nagaoka S, Kusuzaki-Nagira T, Nishihara T, Okahashi N, Koga T. Molecular and immunological characterization of a 64-kDa protein of Actinobacillus actinomycetemcomitans. Oral Microbiol Immunol 1995; 10: 151 /9.
    • 12. Minami J, Matsumoto S, Yamada T. Putative HSP 70 gene from Actinobacillus actinomycetemcomitans molecular cloning and sequence analysis of its gene. Oral Microbiol Immunol 1998; 13: 113 /9.
    • 13. Goulhen F, Hafezi A, Uitto V-J, Hinode D, Nakamura R, Grenier D, Mayrand D. Subcellular localization and cytotoxic activity of the GroEL-like protein isolated from Actinobacillus actinomycetemcomitans. Infect Immun 1998; 66: 5307 /13.
    • 14. Gerner EW, Schneider MJ. Induced thermal resistance in HeLa cells. Nature 1975; 256: 500 /22.
    • 15. Venetianer A, Pirity M, Hever-Szabo A. The function of heatshock proteins in stress tolerance. Cell Biol Int 1994; 18: 605 / 15.
    • 16. Goulhen F, Grenier D, Mayrand D. Stress response in Actinobacillus actinomycetemcomitans : induction of general and stress specific proteins. Res Microbiol 2003; 154: 43 /8.
    • 17. Lowry OH, Rosenbrough NY, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265 /75.
    • 18. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680 /5.
    • 19. Hinode D, Grenier D, Mayrand D. A general procedure for the isolation of HSPs from periodontopathogenic bacteria. J Microbiol Methods 1996; 25: 349 /55.
    • 20. Baab DA, Oberg A, Lundstrom A. Gingival blood flow and temperature changes in young humans with a history of periodontitis. Arch Oral Biol 1990; 2: 95 /101.
    • 21. Marsh P, Martin M. The mouth as a microbial habitat. In: Marsh P, Martin M, eds. Oral Microbiology. New York: Chapman & Hall, 1992: 6 /26.
    • 22. Arguelles JC. Thermotolerance and trehalose accumulation induced by heat-shock in yeast cells of Candida albicans. FEMS Microbiol Lett 1997; 146: 65 /71.
    • 23. Zeuthen ML, Howard DH. Thermotolerance and the heatshock response in Candida albicans. J Gen Microbiol 1989; 135: 2509 /18.
    • 24. Schonfeld SE. Oral microbial ecology. In: Slots J, Taubman MA, eds. Contemporary Oral Microbiology and Immunology. Toronto: Mosby Year Book, 1992: 267 /74.
    • 25. Lu B, McBride BC. Stress response of Porphyromonas gingivalis. Oral Microbiol Immunol 1994; 9: 166 /73.
    • 26. Takahashi N, Yamada T. Acid-induced tolerance and acidogenicity of non-mutans streptococci. Oral Microbiol Immunol 1999; 14: 43 /8.
    • 27. Mayrand D, Gravel C, Grenier D. The response of Fusobacterium nucleatum to heat or pH shock. J Dent Res 2001; 80: 1222.
    • 28. Vayssier C, Mayrand D, Grenier D. Detection of stress proteins in Porphyromonas gingivalis and other oral bacteria by western immunoblotting analysis. FEMS Microbiol Lett 1994; 121: 303 /8.
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