Remember Me
Or use your Academic/Social account:


Or use your Academic/Social account:


You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.


Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message


Verify Password:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Morishita, Y. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article

Classified by OpenAIRE into

mesheuropmc: digestive, oral, and skin physiology, food and beverages, digestive system, fluids and secretions
Food restriction and intestinal bacteria had been reported to have effects on the physiological processes of laboratory animals. Therefore, a study was carried out on the effect of vitamin restriction on the caecal bacteria and short-chain fatty acid (SCFA) concentrations in Wistar rats. In rats fed ad libitum with a purified diet with a 0.3 per cent vitamin mixture, concentrations of bifidobacteria increased significantly (P<0.05) compared with animals fed a 1 per cent and 2 per cent vitamin diet. There was also a trend of increased lactobacilli, but this was not significant. Overgrowth of aerobic bacteria such as enterobacteria and enterococci was not found in the 0.3 per cent group. SCFA concentrations were reduced, but not significantly, in the 0.3 per cent group compared with the 1 per cent and 2 per cent groups. Rats fed a 50 per cent restricted diet containing 1 per cent vitamin mixture had significantly increased concentrations of lactobacilli (P<0.001) and bifidobacteria (P<0.05). In the 2 per cent vitamin regimen only, lactobacilli increased in the 50 per cent restricted group, but not significantly. Significantly decreased concentrations (P<0.01) of staphylococci were found in the animals fed 50 per cent restricted diets compared with the ad libitum groups. SCFA concentrations were reduced (P<0.01) by food restriction with both the 1 and 2 per cent vitamin diets. Growth of caecal lactobacilli was stimulated more by calorie restriction, and growth of bifidobacteria was stimulated more by vitamin restriction. The enhanced growth of fermentative bacteria in the caecal microbiota of rats by vitamin and calorie restriction is discussed in relation to microbial competition or antagonism.Keywords: Vitamin and calorie restriction, Caecal bacteria, Bifidobacteria, Lactobacilli, Short-chain fatty acids, Rat
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1 . Benno Y, Mitsuoka T. (1991). Effect of diet and aging on human fecal microflora. Bijidobacteria and Microjlora 10, 89-96.
    • 2. Cummings JH, Macfarlane GT. (1991). The control and consequences of bacterial fermentation in the human colon. Journal of Applied Bacteriology 70,443459.
    • 3. Donaldson RM. (1964). Normal bacterial populations of the intestine and their relation to intestinal function. New England Journal of Medicine 270, 938-945.
    • 4. Gelmont D, Stein RA, Mead JF. (1981). The bacterial origin of rat breath penthane. Biochemical and Biophysical Research Communications 102, 932-936.
    • 5. Gilman-Sachs A, Kim YB, Pollard M, Snyder DL. (1991). Influence of aging, environmental antigens, and dietary restriction on expression of lymphocyte subsets in germ-free and conventional LobundWistar rats. Journal of Gerontology 46, B101-106.
    • 6. Goldin BR, Gorbach SL. (1992). Probiotics for humans. In: Fuller R (ed) Probiotics. Chapman & Hall, London, pp. 355-376.
    • 7 . Gorbach SL. (1971). Intestinal microflora. Gastroenterology 60, 1 1 10-1 129.
    • 8. Gordon HA, Bruckner-Kardoss E, Wostmann BS. (1966). Aging in germfree mice: life tables and lesions observed at natural death. Journal of Gerontology 21, 380-387.
    • 9. Klurfeld DM, Welch CB, Davis MJ, Kritchevsky D. (1989). Determination of degree of energy restriction necessary to reduce DMBA-induced mammary tumorigenesis in rats during the promotion phase. Journal of Nutrition 119, 286-29 1.
    • 10. Koizumi A, Weindurch R, Walford R. (1987). Influences of dietary restriction and age on liver enzyme activities and lipid peroxidation in mice. Journal of Nutrition 117, 361-367.
    • 11. Laganiere S, Yu BP. (1993). Modulation of membrane phospholipid fatty acid composition by age and food restriction. Gerontology 39, 7-17.
    • 12. Masoro EJ. (1988). Food restriction in rodents: an evaluation of its role in the study of aging. Journal of Gerontology 43, B59-B64.
    • 13. Mata LJ, Mejicanos ML, Jimenez F. (1972). Studies on the indigenous gastro-intestinal flora of Guatemalan children. American Journal of Clinical Nutrition 25, 1380-1390.
    • 14. Mitsuoka T. (1990). Bifidobacteria and their role in human health. Journal of Industrial Microbiology 6, 263-268.
    • 15. Mitsuoka T, Sega T, Yamamoto S. (1965). Eine verbesserte Methodik der qualitativen und quantitativen Analyse der Darmflora von Menschen und Tieren. Zentrlblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abteilung I, Originale A195, 455-469.
    • 16. Morishita Y. (1994). The effect of dietary mannitol on the caecal microflora and short-chain fatty acids in rat. Letters in Applied Microbiology 18, 27-29.
    • 17. Morishita Y, Miyaki K. (1979). Effects of age and starvation on the gastrointestinal microflora and the heat resistance of fecal bacteria in rats. Microbiology and Immunology 23, 455470.
    • 18. Morishita Y, Ohtsuka K , Sawairi Y, Ozawa 0. (1993). Effects of 4'-galactosyllactose on the cecal microflora and short-chain fatty acids in rats. BIFIDUS 7, 21-27 (in Japanase).
    • 19. Morishita Y, Shiromizu K. (1990). Suppressive effect of feeding yoghurt or lactose on N-methylN'-nitro-N-nitrosoguanidine-inducedgastric tumorigenesis in rats. Bijidobacteria and Microjora 9, 135-138.
    • 20. Morishita Y, Yamada H, Shiiba K, Kimura N, Taniguchi H. (1993). Effect of hydrolysate of wheat bran hemicellulose on the cecal microflora and short-chain fatty acid concentrations in rats and mice. Bjfidobacteria and Microjora 12, 19-24.
    • 21. Prins RA. (1977). Biochemical activities of gut micro-organisms. In: Clarke RTJ, Bauchop T (eds) Microbial Ecology of the Gut. Academic Press, London, pp. 73-183.
    • 22. Rowland IR. (1988). Interactions of the gut microflora and the host in toxicology. Toxicologic Pathology 16, 147-153.
    • 23. Smith HW. (1965). Observations on the flora of the alimentary tract of animals and factors affecting its composition. Journal of Pathology and Bacteriology 89, 95-122.
    • 24. Snyder DL, Pollard M, Wostmann BS, Luckert P. (1990). Life span, morphology, and pathology of diet-restricted germ-free and conventional LobundWistar rats. Journal of Gerontology 45, B52-B58.
    • 25. Steinbach G , Kumar SP, Reddy BS, Lipkin M, Holt PR. (1993). Effects of caloric restriction and dietary fat on epithelial cell proliferation in rat colon. Cancer Reseurch 53, 2745-2749.
    • 26. Tannock GW. (1983). Effect of dietary and environmental stress on the gastrointestinal microflora. In: Hentges GJ (ed) Human Intestinal Microjora in Health and Disease. Academic Press, New York, pp. 517-539.
    • 27. Tornqvist M, Gustafsson B, Kautianen A, HarmsRingdahl M, Granath F, Ehrenberg L. (1989). Unsaturated lipids and intestinal bacteria as sources of endogenous production of ethene and ethylene oxide. Carcinogenesis 10, 39-41.
    • 28. Yu BP. (1993). Antioxidant action of dietary restriction in the aging process. Journal of Nutritional Science and Vitaminology 39, S75-S83.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article

Collected from