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Vuyst, Luc De; Makras, Lefteris; Avonts, Lazlo; Holo, Helge; Yi, Qing; Servin, Alain; Fayol-Messaoudi, Domitille; Berger, Cédric; Zoumpopoulou, Georgia; Tsakalidou, Effue; Sgouras, Dionyssios; Martinez-Gonzales, Beatriz; Panayotopoulou, Effie; Mentis, Andreas; Smarandache, Diana; Savu, Lorand; Thonart, Philippe; Nes, Ingolf (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
Subjects:
The EU-funded PROPATH project addresses the important health issue of prevention of gastrointestinal disorders through probiotics and prebiotics. Seven European laboratories are co-operating in this project, which aims to isolate and characterize the relevant antimicrobial agents to combat Gram-negative bacteria including Helicobacter pylori and Salmonella enterica serovar Typhimurium. In this paper, the first results on the screening for probiotic or potentially probiotic lactobacilli that exhibit antimicrobial activity towards these Gram-negative pathogenic bacteria are presented. Spot-on-lawn assays, well-diffusion assays and time-kill studies were performed among the lactic acid bacteria strains that were either collected from fermented foods and faeces (breast-fed babies, infants and animals) or isolated from commercial products to investigate whether any of the collected strains were inhibiting growth of or were killing certain indicator bacteria. Strains inhibiting the gastrointestinal pathogens mentioned above were found. Evidence has been obtained that compounds different from organic acids are produced.Key words: probiotics, antimicrobial agents, gastrointestinal pathogens.

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