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Ter Steeg, P. F.; Van Der Hoeven, J. S.; Bakkeren, J. A. J. M. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
Subjects:

Classified by OpenAIRE into

mesheuropmc: food and beverages, stomatognathic diseases, stomatognathic system
Previous experiments have indicated that the enrichment of subgingival plaque in human serum led to specific consortia of micro-organisms. The present study was undertaken to identify the role which particular organisms have in these consortia in serum degradation. Firstly, isolates from consortia were tested for their ability to degrade immunoglobulin G, the major glycoprotein in serum. From the variety of species tested, only Bacteroides intermedius, Bacteroides oralis and Eubacterium nodatum were capable of IgG-cleavage and protein-core attack. Reconstitutions of consortia showed that without 'cleavers', combinations of saccharolytic specialists such as Bifidobacterium adolescentis and Streptococcus sanguis II and/or of (poly-)peptide- and amino acid-fermenting specialists such as Peptostreptococcus micros, Eubacterium brachy and Fusobacterium nucleatum were incapable of extensive growth on serum and of immunoglobulin A and G consumption. The protein cleaving organisms make glycoproteins accessible as substrates to other members of these consortia. Furthermore, these IgG-cleavers may also enhance growth of others by eliminating host-defence mechanisms in vivo. These consortia seem well adapted to their sites of isolation from various anaerobic body infections.Keywords: Immunoglobulin G; Periodontitis; Serum degradation; Bacteroides intermedius; Bacteroides oralis; Eubacterium nodatum; Peptostreptococcus micros.
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    • I . BeightonD,SmithK, Hayday H. (1986). Thegrowth of bacteria and the production of exoglycosidic enzymes in the dental plaque of macaque monkeys. Archives of Oral Biology 31,829-835.
    • 2. Carlsson J, Hofling JF, Sundqvist GK. (1984). Degradation of albumin, haemopexin, haptoglobin and transferrin, by black-pigmented Bacteroides species. Journal of Medical Microbiology 1 8 , 3 9 4 6 .
    • 3. Hill GB, Ayers OM, Kohan AP. (1987). Characterisitics and sites of infection of Eubacterium nodatum, Eubacterium timidum, Eubacterium brachy, and other asaccharolytic Eubacteria. Journal of Clinical Microbiology 25, 1540-1 545.
    • 4. Hodge JE, Hofreiter BT. (1962). Determination of reducing sugars and carbohydrates. In: Whistler RL, Wolframe ML (eds), Methods in Carbohydrate Chemistry Vol 1, Academic Press, New York, pp 388-389.
    • 5 . Holdeman LV, Cat0 EP, Moore WEC. (1977). Anaerobe laboratory manual, 4th ed. Blacksburg, Virginia, Virginia Polytechnic Institute and State University.
    • 6. Jong MH de, Hoeven JS van der. (1987). The growth of oral bacteria on saliva. Journal of Dental Research 66,498-505.
    • 7. Kilian M, Mestecky J, Russell MW. (1988). Defense mechanism involving Fc-dependent functions of immunoglobulin A and their subversion by bacterial immunoglobulin proteases. Microbiological Reviews 52,296-303.
    • 8. Koch AL, Putnam SL. (1971). Sensitive biuret method for determination of protein in an impure system such as whole bacteria. Analytical Biochemistry 44,239-245.
    • 9. Moore WEC. (1987). Microbiology of periodontal disease.JournalofPeriodontaIResearch22,335-341.
    • 10. Murdoch DA, Mitchelmore IJ, Tabaqchali S. (1988). Peptostreptococcus micros in polymicrobial abscesses. The Lancet 8585(I), 594.
    • 11. Newman HN. (1980). Neutrophils and IgG at the host-plaque interface on children's teeth. Journal of Periodontology 51,642-65 1.
    • 12, Nose M, Wigzell H. (1983). Biological significance of carbohydrate chains on monoclonal antibodies. Proceedings of National Academy of Sciences USA 80,66324636.
    • 13. Steeg P F ter, Hoeven JS van der, Jong MH de, Munster PJJ van, Jansen MJH. (1987). Enrichment of subgingival microflora leading to accumulation of Bacteroides species, Peptostreptococci and Fusobacteria. Antonie van Leeuwenhoek 53,261-272.
    • 14. Steeg PF ter, Hoeven JS van der, Jong MH de, Munster PJJ van, and Jansen MJH. (1988). Modelling the gingival pocket by enrichment of subgingival microflora in human serum in chemostats. Microbial Ecology in Health and Disease l, 73-84.
    • 15. Steeg PF ter, Hoeven JS van der. (1989). Development of periodontal microflora. Microbial Ecology in Health and Disease 2, 1-10,
    • 16. Sundqvist G, Carlsson J, Hermann B, Tarnvik A. (1985). Degradation of human immunoglobulins G and M and complement factors C3 and C5 by blackpigmented Bacteroides. Journal of Medical Microbiology 19,85-94.
    • 17. Tew JG, Marshall DR, Moore WEC, Best AM, Palcanis KG, Ranney RR. (1985). Serum antibody reactivity with predominant organisms in the subgingivalflora of young adultswith generalizedsevere peridontitis. Infection and Immunity 48,303-3 11.
    • 18. Tofte RW, Peterson PK, Schmeling D, Bracke J, Quie PG. (1980). Opsonization of four Bacteroides species: role of the classical complement pathway and immunoglobulin. Infection and Immunity 27, 78&792.
    • 19. Tolo K, Brandtzaeg P. (1982). Relation between periodontal disease activity and serum antibody titers to oral bacteria. In: Genco RJ, Mergenhagen SE (eds), Host-parasite interactions in periodontal disease. American Society for Microbiology, Washington DC. pp 270-282.
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