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Alqumber, Mohammed A.; Burton, Jeremy P.; Devenish, Celia; Tagg, John R. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
Subjects:
The prevalence of Lactobacillus iners is currently difficult to determine since it cannot be propagated on conventional Lactobacillus-selective media. Based on comparative analysis of 16S rRNA gene sequences a simple L. iners-specific PCR method was developed and then applied to screen vaginal, fecal, and saliva samples for this species. Twenty-one (53%) of 40 vaginal specimens were positive, whereas fecal (n=9) and saliva (n=16) samples were PCR-negative for L. iners. These findings support previous molecular and culture-based ecology studies that have indicated L. iners may be relatively more prevalent in the microflora of the human vagina than that of the oral or gastrointestinal cavities.Key words: Lactobacillus iners, species-specific PCR, vaginal colonization

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