LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Mallett, A. K.; Rowland, I. R.; Bearne, C. A.; Flynn, J. C.; Fehilly, B. J.; Udeen, S.; Farthing, M. J. G. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
Subjects:

Classified by OpenAIRE into

mesheuropmc: digestive, oral, and skin physiology, food and beverages
The effect of dietary manipulation on faecal enzyme activities was studied in six healthy volunteers who consumed a mixed, free choice diet (control) or that diet with additional apple pectin (18 g/day), wheat bran (30 g/day) or fat (total greater than 150 g/day) for periods of up to 3 weeks. Control and test diets were alternated over a continuous period of 5 months. Food consumption was recorded and showed that in control periods each subject maintained a high fat (approximately 40 per cent of daily calories) and moderate plant fibre (approximately 18 g/day) intake. Pectin and bran decreased faecal bacterial ß-glucosidase and ß-glucuronidase activities, and pectin increased the stool concentration and rate of formation of ammonia by faecal incubations in vitro, but fat supplementation was without consistent effect. The results demonstrate that plant cell-wall components, but not dietary fat, significantly influenced the enzymic activity of the faecal microflora from subjects maintaining a mixed dietary intake typical of the UK.Keywords: Gut flora; Bran; Pectin; Fat; Ammonia; ß-glucosidase; ß-glucuronidase
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1. Altman D. G., Gore S. M., Gardner M. J., Pocock, S. J. (1983). Statistical guidelines for contributors to medical journals. British Medical Journal 286, 1489-1493.
    • 2. Alvey N., Galwey N., Lane P. (1982). An Introduction to Genstat. Academic Press, London.
    • 3. Attebery H.R., Sutter V. L. ,Finegold S. M. (1 972). Effect of a partially defined diet on normal human fecalflora. American Journal of Clinical Nutrition 25, 1391-1 398.
    • 4. Black A. E. (1982). The logistics of dietary surveys. Human Nutrition and Applied Nutrition MA, 85-89.
    • 5. Bornside G. H., Cohn I. (1965). Stability of normal human fecal flora during a chemically defined, low residue liquid diet. Annals of Surgery 181,58-60.
    • 6. Cole C. B., Fuller R., Mallett A. K., Rowland I. R. (1985).The influenceof the host on the expression of microbial enzyme activities involved in the metabolism of foreign compounds. Journal of Applied Bacteriology 59,549-553.
    • 7. Dawson A. M. (1978). Regulation of blood ammonia. Gut 19,504-509.
    • 8. DeBethizy J. D., Sherril J. M., Rickert D. E., Hamm T. E. (1983). Effects of pectin-containing diets on hepatic macromolecular covalent binding of 2,6- dinitro-[H3]toluene in Fischer F344 rats. Toxicology and Applied Pharmacology 69,369-376.
    • 9. Domellof L., Darby L., Hanson D., Mathews L., Simi B., Reddy B. S. (1982). Fecal sterols and bacterial P-glucuronidase activity: a preliminary metabolic epidemiology study of healthy volunteers from UmeB, Sweden, and metropolitan New York. Nutrition and Cancer 4, 120-127.
    • 10. Drasar B. S., Hill M. J. (eds) (1976). Human Intestinal Flora, 1st edition. Academic Press, London.
    • 11. Drasar B. S., Jenkins D. J. A., Cummings J. H. (1976). The influence of a diet rich in wheat fibre on the human faecal flora. Journal of Medical Microbiology 9,423-43 1.
    • 12. Goldin B. R. (1986). In situ bacterial metabolism and colon mutagens. In: Ornston L. N., Balows A., Bauman P. (eds) Annual Review of Microbiology 40 Annual Reviews Inc., Palo Alto 367-393.
    • 13. Goldin B. R., Adlercreutz H . , Dwyer J . T., Swenson L., Warram J . H., Gorbach S. L. (1981). Effect of diet on excretion of estrogens in pre- and post-menopausal women. Cancer Research 41, 3771-3773.
    • 14. Goldin B. R., Swenson L., Dwyer J., Sexton M., Gorbach S. L. (1980). Effect of diet and Lactobacillus acidophilus supplement on human fecal bacterial enzymes. Journal of the National Cancer Institutes 64,255-260.
    • 15. Holdeman L. V . , Moore W. E. C. (1973). Anaerobe Laboratory Manual, 2nd edn. VPI Anaerobe Laboratory, Blacksburg, VA.
    • 16. Holdeman L. V., Good I . J., Moore W. E. C. (1 976). Human fecal flora: variation in bacterial composition within individuals and a possible effect of emotional stress. Applied and Environmental Microbiology 31,359-375.
    • 17. HMSO (1981). Household Food Consumption and Expenditure. HMSO, London.
    • 18. MacFarlane G. T., Cummings J. H., Allison C. (1986). Protein degradation by human intestinal bacteria. Journal of General Microbiology 132, 1647-1656.
    • 19. Moore W. E. C., Cat0 E. P., Good 1. J., Holdeman L. V. (1981). The effect of diet on the human fecal flora. In: Bruce W. R., Correa P., Lipkin M., Tannenbaum S. R., Wilkins T. D. (eds). Banbury Report 7: Gastrointestinal Cancer-Endogenous Factors. Cold Spring Harbor Laboratory, New York, 11-21.
    • 20. Moore W. E. C., Holdeman L. V. (1974). Special problems associated with the isolation and identification of intestinal bacteria in fecal flora studies. American Journal of Clinical Nutrition 27, 1450-1455.
    • 21. Nanno M., Morotomi M., Takayama H . , Kuroshima T., Tanaka R., Mutai M. (1986). Mutagenic activation of biliary metabolites of benzo[a]pyrene by P-glucuronidase-positive bacteria in human faeces. Journal of Medical Microbiology 22,351-355.
    • 22. Paul A. A., SouthgateD. A. T. (1978). McCance and Widdowson's The Composition of Foods. HMSO, London.
    • 23. Reddy B. S., Hedges A. R., Laaso K., Wynder E. L. (1978). Metabolic epidemiology of large bowel cancer: fecal bulk and constituents of high-risk North American populations and low-risk Finnish population. Cancer 42,2832-2838.
    • 24. Reddy B. S., Wynder E. L. (1973). Large bowel carcinogenesis: fecal constituents of populations with diverse incidence rates of colon cancer. Journal of the National Cancer Institutes 50,1437-1441.
    • 25. Ross J. K . , Leklem J. E. (1981). The effect of dietary citrus pectin on the excretion of human fecal neutral and acid steroids and the activity of 7a-dehydroxylase and P-glucuronidase. American Journal of Clinical Nutrition 34,2068-2017.
    • 26. Rowland I. R., Maliett A. K., Wise A. (1985). The effect of diet on the mammalian gut flora and its metabolic activities. C R C Critical Reviews in Toxicology 16,31-103.
    • 27. Sigma Chemical Co. (1974). The Colorimetric Determination of Urea Nitrogen. Sigma Technical Bulletin no. 640.
    • 28. Snedecor G. W., Cochran W. G. (1980). Statistical Methods, 7th edn. Iowa State University Press, Ames, IA.
    • 29. Stephen A. M., Cummings J. H. (1980). Mechanisms of action of dietary fibre in the human colon. Nature 284,283-284.
    • 30. Takada H., Hirooka T., Hiramatsu Y., Yamamoto M . (1982). Effect of P-glucuronidase inhibitor on azoxymethane-induced colonic carcinogenesis in rats. Cancer Research 42,331-334.
    • 31. Van Soest P. J. (1984). Some physical characteristics of dietary fibres and their influence on the microbial ecology of the human colon. Proceedings of the Nutrition Society 43,25-33.
    • 32. Visek W. J., Kolodny G . M., Gross P. R. (1972). Ammonia effects in cultures of normal and transformed 3T3 cells. Journal of Cellular Physiology 74, 544-548.
    • 33. Wise A,, Mallett A. K., Rowland I. R. (1981). Dietary fibre bacterial metabolism and toxicity of nitrate in the rat. Xenobiotica 12, 111-1 18.
    • 34. Wrong 0. M., Edwards C. J., Chadwick V. S. (1981). The Large Intestine-Its Role in Mammalian Nutrition and Homeostasis. MTP Press, Lancaster.
  • No related research data.
  • No similar publications.

Share - Bookmark

Funded by projects

  • WT

Cite this article

Collected from