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Guliang, Huang; Tefu, Lin (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
M. tuberculosis L-forms researches done in China during the recent years were reviewed in this article. M. tuberculosis L-forms could be produced spontaneously or induced by isoniazid or D-cyclic serine, that influenced the synthesis of cell walls. Among the acid-fast organisms isolated from the patients, more L-forms were found than vegetative forms. In extrapulmonary tuberculosis, M. tuberculosis disseminated through the blood mainly in L-forms, L-forms could adhere on the surface or harbor inside the red cells. With the loss of cell walls, L-forms of M. tuberculosis lost some of their pathogenic features, specific for diagnosis, e.g. OT reaction remained negative and no tuberculosis tubercles formed. In most of these patients only interstitial inflammation could be seen in different organs, similar to that of virus or mycoplasma infection. Unless acid-fast, immunohistochemical staining or isolation was made, it often led to misdiagnosis in histological examination. According to clinical examinations and animal experiments, M. tuberculosis L-forms were found to be closely related to pulmonary carcinoma, especially in adenocarcinoma L-forms were isolated in 34.38% of the cases. M. tuberculosis L-forms were low in pathogenicity, the diseases caused were often escaped from being noticed. M. tuberculosis L-forms were found in 86% of abnormal feeling in nasopharynx, in 32% of chronic prostatitis. M. tuberculosis L-forms were also found in semen of infertile male, the adhesion of L-forms on the sperms inhibited their motility. M. tuberculosis L-forms grew better and faster in liquid than on solid media. The most sensitive method of detection was the using of immunohistochemical technique. In acid-fast staining IK method was better than ZN method. In compensation to the loss of cell walls, the cell membranes became greatly thickened, that increased the difficulty in DNA extraction for PCR examination. Physical grinding could be used to increase the positive rate in detection. M. tuberculosis vegetative forms as well as L-forms proved to be equally important in recognition and elimination of M. tuberculosis. Further study in L-forms of M. tuberculosis is necessary for controlling tuberculosis.Keywords: M. tuberculosis, L-forms, biological characters, pathogenicity.
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    • 1. Anon. Tuberculosis control and research strategies for the 1990's: memorandum from a WHO meeting. Bull WHO 1992; 70:17.
    • 2. Marston BJ, Shinnick TB. Differentially expressed genes of M. tuberculosis. Ann NY Acad Sci 1996; 297: 32.
    • 3. Ma XL, Huang GL, Lin TF. Isolation of M. tuberculosis L-forms from clinical specimens. Acta Acad Med Bengbu 1989; 14 (4): 229 - 31.
    • 4. Mattman LH, Tunstall LH, Methew WW, et al. L-variation in mycobacteria. Am Res Respir Dis 1960; 82 (2): 202 - 11.
    • 5. Ma XL, Huang GL, Lin TF. Induction of mycobacteria L-forms. Chin J Microecol 1989; 1 (2): 8 - 12.
    • 6. Ma XL, Huang GL, Lin TF. Experimental studies on pathogenicity of Mycobacterium tuberculosis L-forms. Chin J Microecol 1995; 7 (1): 1 - 3.
    • 7. Zhang SF, Yao M, Hong XZ, et al. Pathological and clinical misdiagnosis in acid-fast L-forms infection. Chin J Tuberc Repir Dis 1994; 17 (3): 159 - 61.
    • 8. Gong PY, Pan JH, Zhang SF. Clinical infection of M. tuberculosis L-forms in children. Chin J Microecol 1994; 6 (3): 18 - 9.
    • 9. Xiu XR, Cheng JL, Ge H. Misdiagnosis of malignant lymphadenitis in children. Guizhou Med J 1995; 19: 281 - 2.
    • 10. Xu SX, Jiang YM. Abnormal feeling in nasopharynx and L-forms infection. Chin J Microecol 1998; 10 (4A): 30.
    • 11. Xu SX, Yao M, Jiang YM, et al. Misdiagnosis of acid-fast L-forms infection in nasopharynx. Chin J Microecol 1996; 8 (2): 36 - 7.
    • 12. Wang LJ, Wu SB. L-forms of acid-fast organisms and their relation to chronic prostatitis and infertility. Chin J Microecol 1998; 10 (4A): 44.
    • 13. Zhang W, Gao YT. Comparative study on lung cancer and tuberculosis. Chin J Tuberc Respir Dis 1988; 11 (3): 125.
    • 14. Dai YH, Lin TF, Huang GL. A serial study on mycobacteria L-forms. Zhongguo Fanglao Zazhi 1996; (Suppl): 45 - 46.
    • 15. Wang AC, Xie JZ. Significance of M. tuberculosis L-forms in cancer of lungs. Guizhou Med J 1995; 19: 279 - 81.
    • 16. Ho J, Zhang SF, Yao M, et al. Influence of M. tuberculosis L-forms on tumor formation in mice. Guizhou Med J 1995; 19: 278.
    • 17. Zhang SF, Yao M, Wang WY, et al. Acid-fast L-forms in malignant tumor. Chin J Microecol 1994; 6 (6): 57 - 9.
    • 18. Wang WY, Ho J, Zhang SF. Mycobacterium tuberculosis L-forms in malignant lymphoma. Chin J Microecol 1995; 7 (3): 47.
    • 19. Ho MW, Tappeser B. Potential contribution of horizontal gene transfer to transboundary movement of living modified organisms resulting from modern biotechnology. Proceeding of workshop on transboundary movement of living modified organisms, Geneva 1997: 171 - 93.
    • 20. Gao XP, Song XY, Liu MJ. Mycobacterium tuberculosis and its L-forms in blood. Guizhou Med J 1995; 19: 288.
    • 21. Zhu ML, Zhang YH. (Personal communication).
    • 22. Dai YH, Li MJ, Lin TF. Clinical isolation of M. tuberculosis and its L-forms in liquid media. Guizhou Med J 1995; 19: 320 - 1.
    • 23. Guo AH, Cheng H, Zhang GC. Factors in fluencing detection of acid-fast L-forms in tuberculosis lesion. Chin J Microecol 1998; 10 (4A): 29.
    • 24. Liu Y, Lin TF. Studies on morphology and electronmicrographic analysis of M. tuberculosis filamentous L-forms. Chinese J Microbiol Immunobiol 1996; 16 (Suppl 2): 49.
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