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Yousefi-Mashouf, R.; Duerden, B. I.; Eley, A.; Rawlinson, A.; Goodwin, L. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
Samples ofsubgingival plaque from 40 deep (4-7 mm) periodontal pockets in 20 adult patients with periodontal disease before and after periodontal therapy were examined for the presence of Prevotella spp. Special attention was focused on the non-pigmented species. Ten representative colonies (isolates) were selected for identification from each positive sample. Prevotella spp. were isolated from 17 (85 per cent) of the patients studied; 82 non-pigmented Prevotella isolates were obtained from 30 pockets. The commonest species was Pr. buccae with 37 isolates from 12 patients. Pr. veroralis (I4 isolates) was isolated from eight patients, Pr. oris (7) from five patients, Pr. orulis (6) and zoogleoformuns ( 5 ) from four each, Pr. bivia (4) from two patients and Pr. bucwlis ( I ) and Pr. ouloru (1) from one patient each. Several Prevotella spp. were isolated from each patient and more than one species was isolated from all except seven pockets. In four pockets, three different non-pigmented Prevotellu spp. and in six pockets, two non-pigmented species were found. Of the 82 isolates, 51 were from samples taken before treatment and 31 after treatment. Most strains identified from pretreatment samples, in particular strains of Pr. buccae, Pr. zoogleoformans, Pr. bivia and Pr. oralis, were not re-isolated after treatment.Keywords - Prevotella; Periodontal pockets; Sodium (SDS-PAGE).
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