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Van Der Hoeven, J. S.; De Jong, M. H.; Van Nieuw Amerongen, A. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article

Classified by OpenAIRE into

mesheuropmc: stomatognathic system, stomatognathic diseases, fluids and secretions
In this study we have investigated the growth of batch-wise enrichment cultures of supragingival plaque on parotid, (PAR) submandibular-sublingual (SM-SL) and clarified whole (CHW) saliva from one volunteer, who also donated the plaque sample. All three salivas supported rapid growth of a mixed oral microflora. Salivary glycoproteins were completely deglycosylated during growth, while most proteins were left intact. The enrichment cultures on PAR and SM-SL salivas had different microbial compositions. B. oralis was dominant in the culture on PAR saliva, but absent in the culture on SM-SL saliva. In contrast E. lentum was found in a high proportion in SM-SL saliva, but was absent in the culture on PAR saliva. S. mitis was a dominant organism in both cultures, but the isolates from PAR and SM-SL saliva belonged to different biotypes. The enrichment on CHW saliva appeared to have an intermediate composition and most species and biotypes isolated either from the culture on PAR or from SM-SL saliva were found in CHW saliva. The various glycosidase and peptidase activities produced in the enrichment were largely cell-associated and found to be the same as those normally found in saliva and dental plaque in vivo. Taken together the results support the contention that the normal oral microflora maintains itself in the oral cavity by using salivary glycoproteins as a substrate. Moreover saliva seems to act as a selective force in the oral cavity, since different types of glycoproteins as found in PAR and SM-SL saliva supported the growth of different microfloras.Keywords: Saliva; Glycoproteins; Glycosidases; Peptidases; Oral microflora; Streptococci; Bacteroides; Adhesion.
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    • Bayer EA, Lamed R. (1986). Ultrastructure of the cell surface cellulosomeof Clostridium thermocellum and its interaction with cellulose. Journal of Bacteriology 167,828-836.
    • Beckers HJA, Van der Hoeven JS (1982). Growth rates of Actinomyces viscosus and Streptococcus mutans during early colonization of tooth surfaces in gnotobiotic rats. Infection and Immunity 35, 583-5 87.
    • Beighton D, Smith K, Hayday H (1986). The growth of bacteria and the production of exoglycosidic enzymes in the dental plaque of macaque monkeys. Archives of Oral Biology 31,829-835.
    • 4. Beighton D, Smith K, Glenister DA, Salamon K, Keevil CW (1988). Increased degradative enzyme production by dental plaque bacteria in mucinlimited continuous culture. Microbial Ecology in Health and Disease 1,85-94.
    • 5. Brock TD, Smith DW, Madigan MT (1984). Biology of Microorganisms. p. 641442. Prentice-Hall Int., London.
    • 6. Cowman RA, Schaefer SJ, Fitzgerald RJ (1979). Specificityof utilization of human salivary proteins for growth by oral streptococci. Caries Research 13, 181-1 89.
    • 7. De Jong MH, Van der Hoeven JS, Van 0 s JH, Olijve JH (1984). Growth of oral Streptococcus speciesand Actinomyces viscosus in human saliva. Applied and Environmental Microbiology 47,90 1-904.
    • 8. De Jong MH, Van der Hoeven JS, Lukassen JAM (1986). Competition for dietary carbohydrates between streptococci in dental plaque. FEMS Microbiology Ecology 38,341-345.
    • 9. De Jong MH, Van der Hoeven JS, Van 0 s JH (1986). Growth of micro-organisms from supragingival dental plaque on saliva agar. Journal of Dental Research 65,85-88.
    • 10. De Jong MH, Van der Hoeven JS (1987). The growth of oral bacteria on saliva. Journal of Dental Research 66,498-505.
    • 1 1 . Douglas CWI (1983). The binding of human salivary amylase by oral strains of streptococcal bacteria. Archs Oral Biol28,567-573.
    • 12. Frandsen EVG, Theilade E, Ellegaard B, Kilian M. (1986). Proportions and identity of IgAl-degrading bacteria in periodontal pockets from patients with juvenile and rapidly progressive periodontitis. Journal of Periodental Research 21,613-623.
    • 13. Holdeman LV, Cat0 EP, Moore WEC (eds). (1977). Anaerobe Laboratory Manual. 4th ed. Blacksburg Virginia Polytechnic Institute State University Anaerobe Laboratory.
    • 14. Hoskins LC, Boulding ET (1976). A gene interaction in man that affects the fecal population density of certain enteric bacteria. Journal of Clinical Investigation 57,74-82.
    • 15. Laemmli UK (1970). Cleavageof structural proteins during the assembly of the head of bacteriophage T4. Nature 277,68M85.
    • 16. Leach SA,Critchely P. (1966). Bacterial degradation of glycoprotein sugars in human saliva. Nature 209, 506.
    • 17. Leach SA, Hayes ML. (1967). Isolation in pure culture of human oral micoorganisms capable of producing neuraminidase. Nature 216,559-560.
    • 18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951). Protein measurements with the fohn phenol reagent. Journal of Biological Chemistry 193, 265-275.
    • 19. Malamud D (1985). Influenceof salivary proteins on the fate of oral bacteria. In: S.E. Mergenhagen and B. Rosan (eds). Molecular Bases of Oral Microbial Adhesion. Washington, DC: American Society for Microbiology, pp. 117-124.
    • 20. Nakamura M, Slots J (1983). Salivary enzymes. Journal of Periodontal Research 18,559-569.
    • 21. Nieuw Amerongen A van, Vreugdenhill AP, Roukema PA (1977). Electrophoretic isolation and partial characterization of a glycoprotein of the submandibular glands of the mouse. Biochimica Biophysica Acta 495,324-335.
    • 22. Nieuw Amerongen A van, Oderkerk CH, Vreugdenhill AP, Roukema PA (1980).Biochemical and immunochemical Studies of a-amylase from the salivary glands of the mouse. Archives of Oral Biology 24,945-953.
    • 23. Nieuw Amerongen A van, Oderkerk CH, Driessen AA (1987). The role of mucins from human whole saliva in the protection of tooth enamel against demineralization in vitro. Caries Research 21, 297-309.
    • 24. Potier M, Mameli L, Belisle M, Dallaire L, Melancon SB (1979). Fluorometric assay of neuraminidase with a sodium 4-methylumbelliferyla-D-N-acetylneuraminate substrate. Analytical Biochemistry 94,287-296.
    • 25. Reddy MS, Levine MJ, Tabak LA (1982). Structure of the carbohydratechains of the proline-rich glycoprotein from human parotid saliva. Biochemical Biophysical Research Communications 104,882-888.
    • 26. Reddy MS, Levine MJ, Prakobphol A (1985). Oligosaccharide structures of the low-molecularJ. S. VAN DER HOEVEN E T AL. weight salivary mucin from a normal individual and one with cystic fibrosis. Journal of Dental Research 64,33-36.
    • 27. Russell MW (1985). Streptococcus mutans adhesins and salivary proteins. Journal of Dental Research, Spec Suppl64,17 1.
    • 28. Smith K, Beighton D (1986). The effects of the availability of diet on the levels of exoglycosidases in the supragingival plaque of macaque monkeys. Journal of Dental Research 65,1349-1352.
    • 29. Van der Hoeven JS, De Jong MH, Rogers AH (1985). Effect of utilization of substrates on the composition of dental plaque. FEMS Microbiology Ecology 31,129-133.
    • 30. Van der Hoeven JS, De Jong MH, Camp PJM, Van den Kieboom CWA (1985). Competition between oral Streptococcus species in the chemostat under alternating conditions of glucose limitation and excess. FEMS Microbiology Ecology 31, 373-379.
    • 31. Veerman ECI, Suppers RJF, Klein CPAT, De Groot K, Nieuw Amerongen A van. (1987). SDSPAGE analysis of the protein layers absorbing in vivo and in vitro to bone-substituting materials. Biomaterials 8,442448.
    • 32. Warren L. (1959). The thiobarbituric acid assay of sialic acids. Journal of Biological Chemistry 234, 1971-1975.
    • 33. Zacharius RM, Zell TE, Morrison JH, Woodlock JJ (1969). Glycoprotein staining following electrophoresis on acrylamide gels. Analytical Biochemistry 30,148-152.
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