Remember Me
Or use your Academic/Social account:


Or use your Academic/Social account:


You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.


Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message


Verify Password:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Narushima, Seiko; Itoh, Kikuji; Kuruma, Kazuo; Uchida, Kiyohisa (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article

Classified by OpenAIRE into

mesheuropmc: digestive system
Germfree mice were orally inoculated with human intestinal bacteria for which the ability to transform bile acids was confirmed by in vitro screening. Three weeks after inoculation, their caecal bile acids were examined. Free-form bile acids were detected in the caecal contents of gnotobiotic mice associated with deconjugating bacteria, Clostridium ramosum R-18 (above 10%) or extremely oxygen sensitive Clostridium M-7 (3.6%). Deoxycholic acid was observed only in the caecal contents of gnotobiotic mice associated with a combination of deconjugating and 7a-dehydroxylating bacteria, i. e. strain R-18 and Eubacterium lentum-like c-25 (4.3%) or a combination of strain R-18 and unidentified Gram-positive rod strain HD-17 (1.1%). 7-Oxo-deoxycholic acid was detected in the caecal contents of gnotobiotic mice associated with strain M-7 (7α-dehydrogenating in vitro) (1.3%) or strain R-18 plus strain M-7 (2.4%). These results suggest that caecal bile acid composition in gnotobiotic mice reflected the results of bacterial activity in vitro, but bacterial transforming ability itself is insufficient for normal bile acid transformation comparable to that of conventional mice.Key words: caecal bile acids, gnotobiotic mice, human intestinal bacteria.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1. Takano S, Matsushima M, Ertu¨ rk E, Bryan GT. Early induction of rat colonic epithelial ornithine and S-adenosyl-Lmethionine decarboxylase activities by N-methyl-N%-nitroN-nitrosoguanidine or bile salts. Cancer Res 1981; 41: 624 - 8.
    • 2. Rafter JJ, Child P, Anderson AM, Alder R, Eng V, Bruce WR. Cellular Toxicity of fecal water depends on diet. Am J Clin Nutr 1987; 45: 559 - 63.
    • 3. Reddy BS. Nutritional factors and colon cancer. Critical reviews in food science and nutrition 1995; 35: 175 - 90.
    • 4. Reddy BS, Simi B, Patel N, Aliaga C, Rao CV. Effects of amount and types of dietary fat on intestinal bacterial 7a-dehydroxylase and phosphatidylinositol-specific phospholipase C and colonic mucosal diacylglycerol kinase and PKC activities during different stages of colon tumor promotion. Cancer Res 1996; 56: 2314 - 20.
    • 5. Stellwag EJ, Hylemon PB. Purification and characterization of bile salt hydrolase from Bacteroides fragilis subsp. fragilis. Biochim Biophys Acta 1976; 452: 165 - 76.
    • 6. Masuda N. Deconjugation of bile salts by Bacteroides and Clostridium. Microbiol Immunol 1980; 25: 1 - 11.
    • 7. Archer RH, Chong R, Maddox IS. Hydrolysis of bile acid conjugates by Clostridium bifermentans. Eur J Applied Microbiol Biotechnol 1982; 14: 41 - 5.
    • 8. Grill J-P, Schneider F, Crociani J, Ballongue J. Purification and characterization of conjugated bile salt hydrolase from Bifidobacterium longum BB536. Appl Environ Microbiol 1995; 61: 2577 - 82.
    • 9. Takamine F, Imamura T. Isolation and characterization of bile acid 7-dehydroxylating bacteria from human feces. Microbiol Immunol 1995; 39: 11 - 8.
    • 10. Hayakawa S, Hattori T. 7a-dehydroxylation of cholic acid by Clostridium bifermentans strain ATCC 9714 and Clostridium sordellii strain NCIB 6929. FEBS Lett 1970; 6: 131 - 3.
    • 11. Ferrari A, Scolasstico C, Beretta L. On the mechanism of cholic acid 7a-dehydroxylation by a Clostridium bifermentans cell-free extract. FEBS Lett 1977; 75: 166 - 8.
    • 12. Stellwag EJ, Hylemon PB. 7a-dehydroxylation of cholic acid and chenodeoxycholic acid by Clostridium leptum. J Lipid Res 1979; 20: 325 - 33.
    • 13. Hylemon PB, Cacciapuoti AF, White BA, Whitehead TR, Fricke RJ. 7a-dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708. Am J Clin Nutr 1980; 33: 2507 - 10.
    • 14. Archer RH, Maddox IS, Chong R. 7a-dehydroxylation of cholic acid by Clostridium bifermentans. Eur J Appl Microbiol Biotechnol 1981; 12: 46 - 52.
    • 15. Hirano S, Masuda N. Transformation of bile acids by Eubacterium lentum. Appl Environ Microbiol 1981; 42: 912 - 5.
    • 16. Hirano S, Nakamura R, Tamaki M, Masuda N, Oda H. Isolation and characterization of thirteen intestinal microorganisms capable of 7a-dehydroxylating bile acid. Appl Environ Microbiol 1981; 41: 737 - 45.
    • 17. Mitsuoka T, Sega T, Yamamoto S. Eine verbesserte Methodik der qualitativen und quantativen Analyse der Darmflora von Menschen und Tieren. Zentralblatt fu¨ r Bacteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. I. Originale 1965; 195: 455 - 69.
    • 18. Tserng KY, Klein PD. Bile acid sulfates: II. Synthesis of 3-monosulfates of bile acids and their conjugates. Lipids 1979; 13: 479 - 86.
    • 19. Takahashi K, Morotomi M. Absence of cholic acid 7a-dehydroxylase activity in the strains of Lactobacillus and Bifidobacterium. J Dairy Sci 1994; 77: 3275 - 86.
    • 20. Goto J, Hasegawa M, Kato H, Nambara T. A new method for simultaneous determination of bile acids in human bile without hydrolysis. Clin Chim Acta 1978; 87: 141 - 7.
    • 21. Okuyama S, Kokubun N, Higashidate S, Uemura D, Hirata Y. A new analytical method of individual bile acids using high performance liquid chromatography and immobilized 3a-hydroxysteroid dehydrogenase in column form. Chem Lett 1979; 1443 - 6.
    • 22. Batta AK, Salen G, Arora R, Shefer S, Batta M, Person A. Side chain conjugation prevents bacterial 7-dehydroxylation of bile acids. J Biol Chem 1990; 256: 10925 - 8.
    • 23. Gustafsson BE, Midtvedt T, Norman A. Metabolism of cholic acid in germfree animals after the establishment in the intestinal tract of deconjugating and 7a-dehydroxylating bacteria. Acta Pathologica, Microbiologica et Immunologica Scandinavica Section B: Microbiology 1968; 72: 433 - 43.
    • 24. Dickinson AB, Gustafsson BE, Norman A. Determination of bile acid conversion potencies of intestinal bacteria by screening in 6itro and subsequent establishment in germfree rats. Acta Pathologica, Microbiologica et Immunologica Scandinavica Section B: Microbiology 1971; 79: 691 - 8.
    • 25. Kayahara T, Tamura T, Amuro Y, Higashino K, Igimi H, Uchida K. D22b-muricholic acid in monoassociated rats and conventional rats. Lipids 1994; 29: 289 - 96.
    • 26. Koopman JP, Prins RA, Mullink JWMA, Welling GW, Kennis HM, Hectors MPC. Association of germfree mice with the intestinal tract of ''normal'' mice. Zeitschrift fu¨ r Versuchstierkunde 1983; 25: 57 - 62.
    • 27. Koopman JP, Welling GW, Huybregts AWM, Mullink JWMA, Prins RA. Association of germ-free mice with intestinal microfloras. Zeitschrift fu¨ r Versuchstierkunde 1981; 23: 145 - 54.
    • 28. Itoh K, Urano T, Mitsuoka T. Colonization resistance against Pseudomonas aeruginosa in gnotobiotic mice. Lab Anim 1986; 20: 197 - 201.
    • 29. Hirano S, Masuda N. Enhancement of the 7a-dehydroxylase activity of a gram-positive intestinal anaerobe by Bacteroides and its significance in the 7a-dehydroxylation of ursodeoxycholic acid. J Lipid Res 1982; 23: 1152 - 8.
    • 30. Eyssen H, Robben J. The indigenous microflora and the intestinal metabolism of cholesterol, bile acids and steroids. In: Grubb R, Midtvedt T, Norin E, eds. The regulatory and protective role of the normal microflora. Stockholm: Stockton Press, 1989: 71 - 88.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article

Collected from