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Shah, H. N.; Gharbia, S. E.; Al-Jalili, T. A. R.; Nash, R. A.; Seddon, S. V. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article
Subjects:
Cell free extracts from a wide variety of Gram-negative, anaerobic, non-sporeforming rods were screened for dehydrogenase enzymes of carbohydrate and nitrogen metabolism. Four enzymes, malate dehydrogenase, glutamate dehydrogenase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, were found to be of diagnostic importance. The ‘Bacteroides fragilis’ group possessed all four enzymes whereas the ‘B. melaninogenicus-B. oralis’ group and the asaccharolytic, pigmented species were characterised by the presence of only malate and glutamate dehydrogenases. The saccharolytic and asaccharolytic pigmented species could be differentiated by the wide difference in pH optimum for malate dehydrogenase. Fusobacterium species and Leptotrichia buccalis both possessed only glutamate dehydrogenase, but there were differences in enzyme activity between both taxa. Other genera such as Anaerorhabdus, Megamonas, Mitsuokella. Rikenella, Sebaldella and Tissierella had characteristic enzyme profiles. These enzymatic data are of diagnostic value within the Bacteroidaceae and may serve as useful markers for studying the ecological interrelationships of these bacteria.Keywords: Bacteroidaceae; Dehydrogenases; Enzyme patterns.
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    • 1. Collins MD, Shah HN. (1986). Reclassification of Bacteroides praeacutus Tissier (Holdeman and Moore) in a new genus Tissierella as Tissierella praeacuta comb. nov. Systematic and Applied Microbiology 36,461-463:
    • 2. Collins MD, Shah HN. (1987). Recent advances in the chemotaxonomy of the genus Bacteroides. In: Borriello SP, Hardie JM, Draser BS, Duerden BI, Hudson MJ, Lysons RJ (Eds), Recent Advances in Anaerobic Bacteriology. Martinus Nijhoff, Lancester, pp. 249-258.
    • 3. Collins MD, Shah HN, Mitsuoka T. (1985). Reclassification of Bacteroides microfusus (Kaneuchi and Mitsuoka) in a new genus Rikenella, as Rikenella microfusus comb. nov. Systematic and Applied Microbiology 6,79-8 1 .
    • 4. Gharbia SE, Shah HN. (1986).Biochemical properties of Fusobacterium species. M V International Congress of Microbiology. Abstract P.G. 4-10.
    • 5. Haapasalo M, Ranta H, Shah HN, Ranta K, Lounatmaa K. (1984). Isolation and characterisation of a new variant of black pigmented asaccharolytic Bacteroides. FEMS Microbiology letters 23, 269-274.
    • 6. Haapasalo M, Ranta H, Shah HN, Ranta K , Lounatmaa K, Kroppenstedt RM. (1986a). Biochemical and structural characterisation of an unusual group of gram-negative, anaerobic rods from human periapical osteitis. Journal of General Microbiology 132,417-426
    • 7. Haapasalo M, Ranta H, Shah HN, Ranta K, Lounatmaa K, KroppenstedtRM. (1986b).Description of Mitsuokella dentalis sp. nov from dental root canals. International Journal of Systematic Bacteriology 36,566-568.
    • 8. Hamman RH, Werner H. (1981). Presence of diaminopimelic acid in propionate negative Bacteroides sp. and in some butyric acid producing strains. Journal of Medical Microbiology 14,205-212.
    • 9. Holdeman LV, Kelly RW, Moore WEC. (1984). Genus Bacteroides. In; Krieg NR, Holt J G (Eds), Bergey's manual of systematic bacteriology, vol. 1. The Williams and Wilkins Company, Baltimore, pp. 604-63 1.
    • 10. Hyleman PB, Young JL, Roadcap RF, Phibbs PV. (1977). Uptake and incorporation of glucose and mannose by whole cells of Bacteroides thetaiotamicron. Applied and Environmental Microbiology 34, 488- 494.
    • 11. Jantzen E, Hofstad T. (1981). Fatty acids of Fusobacterium species: taxonomic implication. Journal General Microbiology 123,163-1 71.
    • 12. Macy JM, Ljungdahl LG, Gottschalk G. (1978). Pathway of succinate and propionate formation in Bacteroides fragilis. Journal of Bacteriology 134, 8 4 91.
    • 13. Miller TL, Wolin MJ. (1979). Fermentations by saccharolytic intestinal bacteria. American Journal of Clinicaland Nutritional Supplies 32, 164-172.
    • 14. Miyagawa E, Azuma R, Suto T. (1979). Cellular fatty acid composition in gram-negative obligately anaerobic rods. Journal of Applied Microbiology 25, 41-51.
    • 15. Miyagawa E, Azuma R, Suto T. (1981). Peptidoglycan composition of gram-negative obligately anaerobic rods. Journal of General and Applied Microbiology 27, 199-208.
    • 16. Moore WEC, Holdeman LV, Kelly W. (1984). Genus Fusobacterium (Knorr, 1922).In Krieg MR, Holt JC (Eds), Bergey's manual of systematic bacteriology, vol 1. The Williams and Wilkins Company, Baltimore, pp 631-637.
    • 17. Reeves HC, Rabin R, Wegner WS, Ajl SJ. (1971). Assay of enzymes of the tricarboxylic acid and glyoxylate cycles. Methods in Microbiology 6A, 425-462.
    • 18. Rogemond V, Gurnet RMF. (1985). Antigens and enzymes of Bacteroides of the Bacteroides fragilis group compared by crossed immunoelectrophoresis. International Journal of Systematic Bacteriology 35,327-332.
    • 19. Shah HN, Al-Jalili TAR, Elhag KM, Mundegar ZR. (1987). Glucose-6-phosphate dehydrogenase and malate dehydrogenase enzyme electrophoretic patterns amongst strains of Bacteroides fragilis. Journal of General Microbiology 133, 1975-1981.
    • 20. Shah HN, Bonnet R, Mateen B, Williams RAD. (1979). The porphyrin pigmentation of subspeciesof Bacteroides melaninogenicus. Biochemistry Journal 180,45-50.
    • 21. Shah HN, Collins MD. (1980). Fatty acid and isoprenoid quinone composition in the classification of Bacteroides melaninogenicus and related taxa. Journal of Applied Bacteriology 48,75-84.
    • 22. Shah HN, Collins MD. (1981). Bacteroides buccalis sp nov Bacteroides denticola sp nov and Bacteroides pentosaceus sp nov, new species of the genus Bacteroides from the oral cavity. Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene I . Abteilung Originale C2,235-241.
    • 23. Shah HN, Collins MD. (1982a). Reclassification of Bacteroides hypermegas (Harrison and Hansen) in a new genus Megamonas as Megarnonas hypermegas comb. nov. Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene I . Abteilung Originale C 2, 235-241.
    • 24. Shah HN, Collins MD. (1982b). Reclassification of Bacteroides multiacidus (Mitsuoka, Terada, Watanabe, Uchida) in a new genus Mitsuokella, as Mitsuokella multiacidus comb. nov. Zentralblatt fur Bakteriologie und Hygiene I . Abteilung Originale C 3,491-494.
    • 25. Shah HN, Collins MD. (1983). Genus Bacteroides a chemotaxonomical perspective. Journal of Applied Bacteriology 55,403-416.
    • 26. Shah HN, Collins MD. (1986a). Reclassification of Bacteroides furcosus (Holdeman and Moore) in a new genus Anaerorhabdus, as Anaerorhabdus furcosus. Systematic and Applied Microbiology 8,86-88.
    • 27. Shah HN, Collins MD. (1986b). Reclassification of Bacteroides termitidis (Sebald) in a new genus Sebaldella, as Sebaldella termitidis comb. nov. International Journal of Systematic Bacteriology 36, 349-350.
    • 28. Shah HN, Collins MD. (1987). Biochemical and chemical studies on some poorly characterised, nonfermentative Bacteroides species. In: Borriello SP, Hardie JM, Draser BS, Duerden BI, Hudson MJ, Lysons RJ (eds) Recent Advances in Anaerobic Bacteriology. Martinus Nijhoff, Lancaster, pp. 287-289.
    • 29. Shah HN, Williams RAD. (1982). Dehydrogenase patterns in the taxonomy of Bacteroides. Journal of General Microbiology 128,2955-2965.
    • 30. Shah HN, Nash RA, Gharbia SE. (1987). Bacteroides and Fusobacteria. In: Duerden BI, Draser BS (eds) Anaerobes and Human Disease. Edward Arnold (Publishers) Ltd. (in press).
    • 31. Shah HN, Williams RAD. (1987a). Utilisation of glucose and amino acids by Bacteroides intermedius and Bacteroides gingivalis. Current Microbiology 15, 241-246.
    • 32. Shah HN, Williams RAD. (1987b). Catabolism of aspartate and asparagine in Bacteroides intermedius and Bacteroides gingivalis. Current Microbiology 15, 3 13-3 18.
    • 33. Shah HN, Williams RAD, Bowden GH, Hardie J. (1976). Comparison of the biochemical properties of Bacteroides melaninogenicus from human dental plaque and other sites. Journal of Applied Bacteriology 41,473-492.
    • 34. Van Steenbergen TJM, De Soet JJ, de Graaff J. (1979). DNA base composition of various strains of Bacteroides melaninogenicus. FEMS Microbiology Letters 5, 127-130.
    • 35. Van Steenbergen TJM, Vlaanderen CA, de Graaff J. (1982). Deoxyribonucleic acid homologies among strains of Bacteroides melaninogenicus and related species. Journal of Applied Bacteriology 53, 269-276.
    • 36. Vasstrand EN, Jensen HB, Miron T, Hofstad T. (1982). Composition of peptidoglycans in Bacteroidaceae: determination and distribution of lanthionine. Infection and Immunity 36, 114-122.
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