Remember Me
Or use your Academic/Social account:


Or use your Academic/Social account:


You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.


Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message


Verify Password:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Mao, Y.; Yu, J.-L.; Ljungh, Å.; Molin, G.; Jeppsson, B. (2011)
Publisher: Microbial Ecology in Health and Disease
Journal: Microbial Ecology in Health and Disease
Languages: English
Types: Article

Classified by OpenAIRE into

mesheuropmc: food and beverages, bacteria, digestive, oral, and skin physiology
Dietary administration of lactobacilli and certain fibres significantly reduced the severity of methotrexate-induced enterocolitis in rats by improving intestinal mucosal structure, reversing microbiota disruption, possibly also influencing intestinal mucosal immunity. The purpose of this study was to evaluate the effects of two Lactobacillus strains and fibres, which have been proven to be effective in enterocolitis of rats, on gut immune response. All rats received continuous intragastric infusion of an elemental diet with or without supplementation of pectin, oatbase, and Lactobacillus reuteri R2LC or Lactobacillus plantarum DSM 9843 from the beginning of the study via a gastrostomy. The control group rats had normal chow throughout the study. On day three, animals received intraperitoneal injections of either methotrexate, 20 mg/kg, or normal saline, and the sampling was done on day six. Administration of methotrexate significantly diminished intestinal secretory IgA level and gut lamina propria CD4/CD8 lymphocyte counts. The addition of L. reuteri R2LC, and L. plantarum DSM 9843, but not of pectin and oatbase, significantly increased the ileal and colonic secretory IgA level, both in soluble and insoluble fractions, and elevated CD4 and CD8 numbers compared with control enterocolitis rats. The enhancement of gut immune function by lactobacilli administration may be one of the important processes through which certain strains of lactobacilli facilitated the recovery from enterocolitis.Keywords: intestinal immunity, enterocolitis, Lactobacillus reuteri, Lactobacillus plantarum, pectin, oat.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1 Alverdy JC. (1990). Effects of glutaminesupplemented diets on immunology of the gut. Journal of Parenteral and Enteral Nutrition 14 suppl4, 109-1 13.
    • 2. Alverdy JC, Aoys E, Weiss-Carrington P, Burke DA. (1992). The effect of glutamine-enriched TPN o n gut immune celiularity. Journul of Surgical Research 52, 34-38.
    • 3. Brandtzaeg P, Halstensen TS, Kett K, Krajci P, Kvale D, Rogmun TO, Scott H, Sollid LM. (1989). Immunobiology and immunopathology of human gut mucosa: Humoral immunity and intraepithelial lymphocytes. Gastroenterology 97, 1562- 1584.
    • 4. Burke DJ, Alverdy JC, Aoys E, Moss GS. (1989). Glutamine-supplemented total p a r e n t e d nutrition improves gut immune function. Archives of Surgery 124, 1396-1399.
    • 5. Char S, Farthing MJG. (1994). Bacteria and gut immunity. Current Opinion in Gastroenterology 10, 659-663.
    • 6. Craven DE, Steger KA, La Chapelle R, Allen DM. (1994). Factitious HIV infection: the importance of documenting infection. Annals of Internal Medicine 15,763-766.
    • 7. Fabia R, Ar'Rajab A, Johansson ML, Willen R, Anderson R, Molin G , Bengmark S. (1993). The effect of exogenous administration of Lactobacillus reuteri R2LC and oat fiber on acetic acid-induced colitis in the rat. Scandinavian Journal of Gastroenterology 28, 155-162.
    • 8. Falini B, Taylor CR. (1983). New developments in immunoperoxidase techniques and their application. Archives of Pathology and Laboratory Medicine 107, 105-109.
    • 9. Fox AD, Kripke SA, DePaula J, Berman JM, Settle BG, Rombeau JL. (1988). Effect of a glutamine-supplemented enteral diet on methotrexate-induced enterocolitis. Journal of Parenteral and Enteral Nutrition 12, 325-33 1.
    • LO. Hirvonen M, Koskinen S, Tolo H. (1993). A sensitive enzyme immunoassay for the measurement of low concentrations of IgA. Journal of Immunological Methodr 163, 59-65.
    • 11. Johansson ML, Molin G, Jeppsson B, Nobaek S, Ahrne S, Bengmark S. (1993). Administration of different Lactobacillus strains in fermented oatmeal soup: In vivo colonization of human intestinal mucosa and effect on the indigenous flora. Applied and Environmental Microbiology 59, 15-20.
    • 12. Jones AL, Hunling S , Hradek GT. (1982). Uptake and intracellular deposition of IgA by rat hepatocytes in monolayer cultures. Hepatology 2, 769- 775.
    • 13. Kagnoff MF. (1987). Immunology of the digestive system. In: Johnson LR (ed), Physiology of the Gastrointestinal Tract, 2nd edn. Raven Press, New York, pp. 1699-1728.
    • 14. Mackall CL, Fleisher TA, Brown MR, Andrich MP, Chen CC, Feuerstein IM, Horowitz ME, Magrath IT, Shad AT, Steinberg SM, Wexier LH, Cress RE. (1995). Age, thymopoiesis, and CD4+ T-lymphocyte regeneration after intensive chemotherapy. New England Journal Medicine 332, 143- 149.
    • 15. Mao Y, Kasravi B, Nobaek S, Wang LQ, Adawi D, Roos G, Stenram U, Molin G, Bengmark S, Jeppsson B. (1996). Pectin-supplemented enteral diet reduces the severity of methotrexate-induced enterocolitis in rats. Scantiinaviun Journal of Gastroenterology 31, 558-567.
    • 16. Mao Y, Nobaek S, Ksaravi B, Adawi D, Stenram U, Molin G, Jeppsson B. (1996). The effects of Lactobucillus strains and oat fiber on methotrexateinduced enterocolitis in rats. Gastroenterology 111, 334-344.
    • 17. McGhee JR, Mestecky J, Elson CO, Kiyono H. (1989). Regulation of IgA synthesis and immune response by T-cell and interleukins. Journal of Clinical Immunology 9, 175-1 99.
    • 18. Mestecky J, Russel MW, Jackson S. (1986). The human IgA system: A reassessment. Clinical imrniinopathology 40, 150-1 14.
    • 19. Molin G, Anderson R, Ahrne S, Lonner C, Marklinder I, Johansson M-L, Jeppsson B, Hengmark S . (1992). Effect of fermented oatmeal soup on the cholesterol level and the Lactobacillus colonization of rat intestinal mucosa. Antonie Van Leeuwenhoek International Journal of General and ikiolecular Microbiology 61, 167-1 73.
    • 20. Namba J, Hidaka Y, Taki K, Morimoto T. (1981). Effect of oral administration of lysozyme or digested bacterial cell walls on immunostimulation in guinea pigs. Infection and Immunity 31, 580.
    • 21. Perdigon G, Nader De Macias ME, Alvarez S, Oliver G, Pesce De Ruiz Holgado A. (1988). Systemic augmentation of the immune response in mice by feeding fermented milks with Lactobacillus casei and Lnctobacillus acidophilus. Immunology 63, 17-23.
    • 22. Perdigon G, Alvarez S, Nadar De Macias ME, Roux ME, Pesce De Ruiz Holgado A. (1990). The oral administration of lactic acid bacteria increases the mucosal intestinal immunity in response to enteropathogens. Journal of Food Protection 53, 404-410.
    • 23. Perdigon G, Alvarez S, Pesce De Ruiz Holgado A. (1991). Immunoadjuvant activity of oral Lactobacillus casei: influence of dose on the secretory immune response and protective capacity in intestinal infections. Journal of Dairy Research 58, 485-496.
    • 24. Rule SA, Hooker M, Costello C, Luck W, H o f i r a n d AV. (1994). Serum vitamin B12 and transcobalamin levels in early HIV disease. American Journal of Hemutolcgy 47, 167-17 1.
    • 25. Sat0 K, Saito H, Tomioka H. (1988). Enhancement of host resistance against Listeriu infection by Lactobacillus ccisei: activation of liver macrophages. Microbiology and Immunology 32,689-698.
    • 26. Slobodianik N, Langini S, Rio ME, Sanahuja JE, Lopez MC, Roux ME. (1984). Effect of casein on the recovery of lymphoid organs surface and intracellular markers in growing rats. Coinmimicationes Biologicas 3, 19I .
    • 27. Spaeth G, Gottwald T, Haas W, Holmer M. (1993). Glutamine peptide does not improve gut barrier function and mucosal immunity in total parenteral nutrition. Journal of Parenteral and Enteral Nutrition 17, 317-323.
    • 28. Williams RC, Gibbons RJ. (1972). Inhibition of bacterial adherence by secretory immunoglobulin A: A mechanism of antigen disposal. Science 177, 697-699.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article

Collected from