LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Parascandolo, James S.; Havemann, Judith; Potter, Helen K.; Huang, Fanglu; Riva, Elena; Connolly, Jack; Wilkening, Ina; Song, Lijiang; Leadlay, P. F.; Tosin, Manuela (2016)
Publisher: Wiley - V C H Verlag GmbH & Co. KGaA
Journal: Angewandte Chemie (International Ed. in English)
Languages: English
Types: Article
Subjects: Iterative Polyketidkatalyse, Communications, Zuschrift, Zuschriften, iterative polyketide catalysis, chemical probes, Chemische Sonden, Communication, dehydratase, QP
Abstract Chemical probes capable of reacting with KS (ketosynthase)‐bound biosynthetic intermediates were utilized for the investigation of the model type I iterative polyketide synthase 6‐methylsalicylic acid synthase (6‐MSAS) in vivo and in vitro. From the fermentation of fungal and bacterial 6‐MSAS hosts in the presence of chain termination probes, a full range of biosynthetic intermediates was isolated and characterized for the first time. Meanwhile, in vitro studies of recombinant 6‐MSA synthases with both nonhydrolyzable and hydrolyzable substrate mimics have provided additional insights into substrate recognition, providing the basis for further exploration of the enzyme catalytic activities.

Share - Bookmark

Funded by projects

  • RCUK | Synthetic probes of natura...

Cite this article