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Parascandolo, James S.; Havemann, Judith; Potter, Helen K.; Huang, Fanglu; Riva, Elena; Connolly, Jack; Wilkening, Ina; Song, Lijiang; Leadlay, P. F.; Tosin, Manuela (2016)
Publisher: Wiley - V C H Verlag GmbH & Co. KGaA
Languages: English
Types: Article
Subjects: QP
Chemical probes capable of reacting with KS (ketosynthase)-bound biosynthetic intermediates were utilized for the investigation of the model type I iterative polyketide synthase 6-methylsalicylic acid synthase (6-MSAS) in vivo and in vitro. From the fermentation of fungal and bacterial 6-MSAS hosts in the presence of chain termination probes, a full range of biosynthetic intermediates was isolated and characterized for the first time. Meanwhile, in vitro studies of recombinant 6-MSA synthases with both nonhydrolyzable and hydrolyzable substrate mimics have provided additional insights into substrate recognition, providing the basis for further exploration of the enzyme catalytic activities.
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