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Honeychurch, K. C. (2016)
Publisher: Scientific & Academic Publishing
Languages: English
Types: Article
The illegal use of 2,4-dinitrophenol (24DNP) as a diet aid has increased markedly resulting in a number of deaths. This paper describes the development of a simple voltammetric method for the measurement of 24DNP in serum at glassy carbon electrode (GCE). It is believed that this is the first report on the voltammetric determination of 24DNP in any biological sample. Initial investigations were undertaken using cyclic voltammetry to characterise the redox behaviour of 24DNP. Over the pH range 2 to 10 four pH dependent reduction peaks were recorded on the initial negative going scan, concluded to result from the reduction of the two nitro groups to the corresponding hydroxylamines. On the return positive going scan two oxidation peaks were recorded, resulting from the oxidation of the hydroxylamine (O1) formed on the initial negative scan and the direct oxidation of the phenol group (O2). At pH 6, the peak potential of the oxidation process O1 occurred at a potential close to 0 V and was chosen for investigation. The optimum voltammetric conditions required were identified to be supporting electrolyte of 0.1 M pH 6.0 phosphate buffer containing 10 % acetonitrile. Using differential pulse voltammetry, the calibration plot was found to be linear from 180 ng/mL to 184 µg/mL (R2= 0.9996), with a detection limit of 98.4 ng/mL (based on a signal-to-noise ratio of 3). The optimised method was evaluated by carrying out 24DNP determinations on spiked and unspiked serum samples. Using an external calibration technique, mean recoveries of 79.2 % were obtained and coefficients of variation of 7.4 % were calculated for a forensically relative con-centration of 36.8 µg/mL. The performance characteristics show that the method holds promise and reliable data may be obtained for 24DNP in forensics and bioanalysis.
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    • Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological Profile for Dinitrophenols. Public Health Service, U.S. Department of Health and Human Services, Atlanta, GA. 1995.
    • Toxicol. Pharmacol. 48 (2007) 115-117.
    • [15] [16] J. Parascandola, Dinitrophenol and bioenergetics: An historical perspective, Mol. Cell. Biochem. 5 (1974) 69-77.
    • A. Petróczi, J.A.V. Ocampo, I. Shah, C. Jenkinson, R. New, R.A. James, G. Taylor, D.P. Naughton, Russian roulette with unlicensed fat-burner drug 2,4-dinitrophenol (DNP): evidence from a multidisciplinary study of the internet, bodybuilding supplements and DNP users, Subst. Abuse Treat.
    • Prev. Policy 10 (2015) 39.
    • Wood, 2,4-Dinitrophenol (DNP): A Weight Loss Agent with Significant Acute Toxicity and Risk of Death, J. Med. Toxicol. 7 (2011) 205-212.
    • H.C.H. Lee, C.Y. Law, M.L. Chen, Y.H. Lam, A.Y.W. Chan, T.W.L. Mak, 2,4-Dinitrophenol: A threat to Chinese body-conscious groups, J. Chin.
    • Med. Assoc. 77 (2014) 443-445.
    • Gary, B.K. Logan, Two deaths attributed to the use of 2,4-dinitrophenol, J. Anal. Toxicol. 30 (2006) 219-222.
    • J. Bartlett, M. Brunner, K. Gough, Deliberate poisoning with dinitrophenol (DNP): an unlicensed weight loss pill, Emerg. Med. J. 27 (2010) 159-160.
    • S. Cotton, 2,4-Dinitrophenol, Chemistry World, 2015, http://www.rsc.org/chemistryworld/2015/08/dinitr ophenol-podcast-weight-loss, accessed 6/11/15.
    • A. Tewari, A. Ali, A. O'Donnell, M.S. Butt, Weight loss and 2,4-dinitrophenol poisoning, BJA 102 (2009) 566-567.
    • Vale, H.K.R. Thanacoody, S. Hill, S.H.L. Thomas, Increasing frequency of severe clinical toxicity after use of 2,4-dinitrophenol in the UK: a report from the National Poisons Information Service, Emerg. Med. J. 32 (2015) 383-386.
    • F. Zack, V. Blaas, C. Goos, D. Rentsch, A. Büttne, Death within 44 days of 2,4-dinitrophenol intake, Int. J. Legal Med. 130 (2016) 1237-1241.
    • B. Hoxha, A. Petróczi, Playing with fire? Factors influencing risk willingness with the unlicensed fat burner drug 2,4-Dinitrophenol (DNP) in young adults, Public Health, 129 (2015) 1519-1522.
    • C. Mathers, D.A McKeown, J. Button, T.D Lee, D.W Holt, 2,4-Dinitrophenol: A dietary supplement that “blows” you away, http://www.the-ltg.org/data/uploads/posters/dnp.pd f accessed 13/1/15.
    • X.-H. Zhao, J.-K. Jiang, Y.-Q. Lu, Evaluation of efficacy of resin hemoperfusion in patients with acute 2,4-dinitrophenol poisoning by dynamic monitoring of plasma toxin concentration, J.
    • Zhejiang Univ. Sci. B. 16 (2015) 720-726.
    • K.C. Honeychurch, G.C. Smith, J.P. Hart, Voltammetric behavior of nitrazepam and its determi[28] [29] [30] [31] [32] [33] [34] [35] [36] [37] [38] 2,4-dinitrophenol in surface water samples based on hydrophilic molecularly imprinted polymers/nickel fiber electrode, Biosens. Bioelectron.
    • Y. Liu, L. Zhu, Y. Zhang, H. Tang, Electrochemical sensoring of 2,4-dinitrophenol by using composites of graphene oxide with surface molecular imprinted polymer, Sensor. Actuat. B-Chem.
    • X. Lü, Z. Wu, J. Shen, J. Feng, Y. Wang, Y. Song, Electrochemical Behavior of 2,4-Dinitrophenol at Multi-Walled Carbon Nanotube-Modified Glassy Carbon Electrode and Its Application, Int. J. Electrochem. Sci. 8 (2013) 2229-2237.
    • X.G. Wang, Y.J. Fan, Z.X. Hao, L.H. Gan, Voltammetric determination of 2,4-dinitrophenol and 2,5-dinitrophenol using a poly-aspartic acid modified electrode, Russ. J. Electrochem. 46 (2010) 1402-1407.
    • H. Yin, Y. Zhou, R. Han, Y. Qiu, S. Ai, L. Zhu, Electrochemical oxidation behavior of 2,4-dinitrophenol at hydroxylapatite film-modified glassy carbon electrode and its determination in water samples, J. Solid State Electrochem. 16 (2012) 75-82.
    • Peckova, V. Vyskocil, The Use of Silver Solid Amalgam Working Electrode for Determination of Nitrophenols by HPLC with Electrochemical Detection, Electroanalysis, 21 (2009) 303-308.
    • J. Karaová, J. Barek, K. Schwarzová-Pecková, Oxidative and Reductive Detection Modes for Determination of Nitrophenols by High-performance Liquid Chromatography with Amperometric Detection at a Boron Doped Diamond Electrode, Anal. Lett. 49 (2016) 66-79.
    • J. Ruana, I. Urbe, F. Borrull, Determination of phenols at the ng/L level in drinking and river waters by liquid chromatography with UV and electrochemical detection, J. Chromatogr. A, 655 (1993) 217-226.
    • E.A. Hutton, B. Ogorevc, M.R. Smyth, Cathodic electrochemical detection of nitrophenols at a bismuth film electrode for use in flow analysis, Electroanalysis, 16 (2004) 1616-1621.
    • Y. Nia, L. Wanga, S. Kokot, Simultaneous determination of nitrobenzene and nitro-substituted phenols by differential pulse voltammetry and chemometrics, Anal. Chim. Acta 431 (2001) 101-113.
    • J. Barek, H. Ebertova, V. Mejstrik, J. Zima, Determination of 2-Nitrophenol, 4-Nitrophenol, 2-Methoxy-5-Nitrophenol, and 2,4-Dinitrophenol by Differential Pulse Voltammetry and Adsorptive Stripping Voltammetry, Collect. Czech. Chem.
    • Commun. 59 (1994) 1761-1771.
    • Chem. 381 (2005) 464-470.
    • J. Musilov, J. Barek, K. Peckov, Determination of Nitrophenols in Drinking and River Water by Differential Pulse Voltammetry at Boron-Doped Diamond Film Electrode, Electroanalysis 23 (2011) 1236-1244.
    • N. Lezi, A. Economou, J. Barek, M. Prodromidis, Screen-Printed Disposable Sensors Modified with Bismuth Precursors for Rapid Voltammetric Determination of 3 Ecotoxic Nitrophenols, Electroanalysis, 26 (2014) 766-775.
    • E.V. Emelyanova, A.N. Reshetilov, Rhodococcus erythropolis as the receptor of cell-based sensor for 2,4-dinitrophenol detection: effect of 'co-oxidation' Process Biochem. 37 (2002) 683-692.
    • S. Bollo, L.J. Nunez-Vergara, M. Bonta, G. Chauviere, J. Perie, J.A. Squella, Cyclic voltammetric studies on nitro radical anion formation from megazol and some related nitroimidazole derivatives J. Electroanal. Chem. 511 (2001) 46-54.
    • F. Taha, H.A. El Shayeb, A.A. Abd El Gaber, A.M. El Roudi, The Polarographic Behaviour of 2,4-Dinitrophenol in Aqueous and non-Aqueous Methanolic Media, Bull. Soc. Chim. Belg. 95 (1986) 229-239.
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