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Smith, G.; Reynolds, M.; Burton, F.L.; Kemi, O.J. (2010)
Publisher: Academic Press
Languages: English
Types: Article
Subjects: QH301

Classified by OpenAIRE into

ACM Ref: TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY
This chapter compares the imaging capabilities of a range of systems including multiphoton microscopy in regard to measurements of intracellular Ca2+ within living cells. In particular, the excitation spectra of popular fluorescent Ca2+ indicators are shown during 1P and 2P excitation. The strengths and limitations of the current indicators are discussed along with error analysis which highlights the value of matching the Ca2+ affinity of the dye to a particular aspect of Ca2+ signaling. Finally, the combined emission spectra of Ca2+ and voltage sensitive dyes are compared to allow the choice of the optimum combination to allow simultaneous intracellular Ca2+ and membrane voltage measurement.
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