Remember Me
Or use your Academic/Social account:


Or use your Academic/Social account:


You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.


Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message


Verify Password:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Dias, Irundika H.K.; Mistry, Jayna; Fell, Shaun; Reis, Ana; Spickett, Corinne M.; Polidori, Maria C; Lip, Greg Y.H.; Griffiths, Helen R. (2014)
Publisher: Elsevier Science
Journal: Free Radical Biology & Medicine
Languages: English
Types: Article
Subjects: CTB, cholera toxin B, PBS, phosphate-buffered saline, SM, sphingomyelin, GSH, glutathione, BCA, bicinchoninic acid, BSO, buthionine sulfoximine, GSSG, oxidized glutathione, AD, Alzheimer disease, BHT, butylated hydroxytoluene, Physiology (medical), ASMase, acid sphingomyelinase, EGTA, ethylene glycol tetraacetic acid, Redox, MDA, malondialdehyde, oxLDL, oxidized low-density lipoprotein, PBST, phosphate-buffered saline with 1% Tween 20, APP, amyloid precursor protein, SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, Original Contribution, EDTA, ethylenediamine tetraacetic acid, Cholesterol, 27OH-C, 27-hydroxycholesterol, PVDF, polyvinylidene fluoride, BACE1, Free radicals, Lipid oxidation, Aβ, β-amyloid, BBB, blood–brain barrier, NAC, N-acetylcysteine, LDL, low-density lipoprotein, Low-density lipoprotein, CSF, cerebrospinal fluid, Lipid raft, Biochemistry, BACE, β-secretase β-site amyloid cleaving enzyme, E-64, proteinase inhibitor E-64, GCL, glutamate–cysteinyl ligase, Aging, ESI–MS, electrospray ionization mass spectrometry, GSH, ELISA, enzyme-linked immunosorbent assay, OxLDL

Classified by OpenAIRE into

mesheuropmc: lipids (amino acids, peptides, and proteins)
Elevated total cholesterol in midlife has been associated with increased risk of dementia in later life. We have previously shown that low-density lipoprotein (LDL) is more oxidized in the plasma of dementia patients, although total cholesterol levels are not different from those of age-matched controls. β-Amyloid (Aβ) peptide, which accumulates in Alzheimer disease (AD), arises from the initial cleavage of amyloid precursor protein by β-secretase-1 (BACE1). BACE1 activity is regulated by membrane lipids and raft formation. Given the evidence for altered lipid metabolism in AD, we have investigated a mechanism for enhanced Aβ production by SH-SY5Y neuronal-like cells exposed to oxidized LDL (oxLDL). The viability of SH-SY5Y cells exposed to 4 μg oxLDL and 25 μM 27-hydroxycholesterol (27OH-C) was decreased significantly. Lipids, but not proteins, extracted from oxLDL were more cytotoxic than oxLDL. In parallel, the ratio of reduced glutathione (GSH) to oxidized glutathione was decreased at sublethal concentrations of lipids extracted from native and oxLDL. GSH loss was associated with an increase in acid sphingomyelinase (ASMase) activity and lipid raft formation, which could be inhibited by the ASMase inhibitor desipramine. 27OH-C and total lipids from LDL and oxLDL independently increased Aβ production by SH-SY5Y cells, and Aβ accumulation could be inhibited by desipramine and by N-acetylcysteine. These data suggest a mechanism whereby oxLDL lipids and 27OH-C can drive Aβ production by GSH depletion, ASMase-driven membrane remodeling, and BACE1 activation in neuronal cells. © 2014 The Authors.

Share - Bookmark

Funded by projects


Cite this article