LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Bisby, RH; Arvanitidis, M; Botchway, CW; Clark, IP
Publisher: Royal Society of Chemistry
Languages: English
Types: Article
Subjects: other, QD
Identifiers:doi:10.1039/b206848f
It is reported (Shear et al, Photochem.Photobiol. 65, 931 (1997) ) that multiphoton near infrared excitation of 5-hydroxytryptophan results in a transient product with green fluorescence. Visible fluorescence from multiphoton excitation enables detection of 5 hydroxytryptophan with extremely high sensitivity and also has potential applications in imaging of biological systems and investigation of protein dynamics. The characteristic fluorescence at 500 nm has now also been observed on one-photon photolysis of solutions at 308 nm, followed by excitation of fluorescence at 430 nm. Fluorescence was observed in aerated and deaerated solutions and in the presence of ascorbate. Enhancement of fluorescence was observed on addition of ethanol. Transient absorption experiments with 308 nm photolysis showed the formation of three transient species. In the presence of ascorbate the radical formed by photoionisation was removed, revealing a long-lived species (τ > 1 ms)) with a similar absorption spectrum, which is ascribed to the fluorescing species. Fluorescence induced by multiphoton excitation had a lifetime of 910 ± 10 ps and was also unaffected by ascorbate. In the presence of organic solvents there was an increase in fluorescence lifetime, but a decrease in overall fluorescence intensity. The fluorescence intensity and fluorescence lifetime both decreased in acidic solution (pH < 3). It is suggested that the fluorescence originates not from the 5-indoxyl radical, but from a transient product formed by intramolecular rearrangement.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1. C.Xu, W.Zipfel, J.B.Shear, R.M.Williams and W.W.Webb, Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy, Proc.Natl.Acad,Sci. USA, 1996, 93, 10763-10768.
    • 2. B.R.Masters, P.T.C.So, K.H.Kim, C.Beuhler and E.Gratton, Multiphoton excitation microscopy, confocal microscopy, and spectroscopy of living cells and tissues, Adv.Enzymol., 1999, 307, 513-536.
    • 3. D.J.S.Birch, Multiphoton excited fluorescence spectroscopy of biomolecular systems, Spectrochim. Acta A, 2001, 57, 2313-2336.
    • 4. J.B.S.Shear, C.Xu and W.W.Webb, Multiphoton-excited visible emission by serotonin solutions, Photochem.Photobiol., 1997, 65, 931-936.
    • 5. a) M.L.Gostkowski, J.Wei and J.B.Shear, Measurements of serotonin and related indoles using capillary electrophoresis with multiphoton-induced hyperluminescence, Anal.Biochem., 1998, 260, 244-250. b) M.L.Gostkowski, J.B.McDoniel, J.Wei, T.E.Curey and J.B.Shear, Characterizing spectrally diverse biological chromophore using capillary electrophoresis with multiphoton-excited fluorescence, J.Amer.Chem.Soc., 1998, 120, 18-22.
    • 6. S.W.Botchway, A.W.Parker, I.Barba and K.Brindle, Development of a time-correlated single photon counting multiphoton laser scanning confocal microscope, Central Laser Facility Annual Report, 2000-2001, RAL-TR-2001-030:pp. 170-171.
    • 7. M.L.Gostkowski, T.E.Curey, E.Okerberg, T.J.Kang, D.A.V.Bout and J.B.Shear, Effects of molecular oxygen on multiphoton-excited photochemical analysis of hydroxyindoles, Anal.Chem., 2000, 72, 3821-3825.
    • 8. S.Udenfriend, D.F.Bogdanski and H.Weissbach, Fluorescence characteristics of 5- hydroxytryptamine (serotonin), Science, 1955, 122, 972-973.
    • 9. S.V.Jovanovic, S.Steenken and M.G.Simic, One-electron reduction potentials of 5- indoxyl radicals, J.Phys.Chem., 1990, 94, 3583-3588.
    • 10. S.V.Jovanovic and M.G.Simic, Tryptophan metabolites as antioxidants, Life Chem.Rep., 1985, 3, 124-130.
    • 12. S.V.Jovanovic, S.Steenken and M.G.Simic, Kinetics and energetics of one-electron transfer reactions involving tryptophan neutral and cation radicals, J.Phys.Chem., 1991, 95, 684-687.
    • 13. A.T.Al-Kawini, P.O'Neill, G.E.Admas, R.B.Cundall, A.Junino and J.Maignan, Characterisation of the intermediates produced on one-electron oxidation of 4-, 5-, 6-, and 7-hydroxyindoles by the azide radical, J.Chem.Soc.Perkin Trans. 2, 1992, 657-661.
    • 14. R.H.Bisby and A.W.Parker, Reactions of triplet duroquinone with -tocopherol and ascorbate: a nanosecond laser flash photolysis and time-resolved resonance Raman investigation, J.Amer.Chem. Soc., 1995, 117, 5664-5670.
    • 15. G.E.Walrafen, Raman spectral studies of water structure, J.Chem.Phys., 1964, 40, 3249- 3256.
    • 16. R.H.Schuler, Oxidation of ascorbate anion by electron transfer to phenoxyl radicals, Radiat.Res., 1977, 69, 417-433.
    • 17. M.C.Depew, M.T.Craw, K.MacCormick and J.K.S.Wan, A CIDEP and fluorescence study of the oxidation of vitamin E: the vitamin E radical cation, Journal of Photochemistry and Photobiology, B: Biology, 1987, 1, 229-239
    • 18. N.Ichinose, S.Tojo and T.Majima, Fluorescence measurement of 3,5-dimethoxyphenol radical cation generated by pulse radiolysis in 1,2-dichloromethane, Chemistry Letters 2000, 1126-1127.
    • 19. M.J.Gordon, E.Okerberg, M.L.Gostkowski and J.B.Shear, Electrophoretic characterization of transient photochemical reaction products, J.Amer.Chem.Soc., 2001, 123, 10780-10781.
    • 20. D.A.Armstrong and P.S.Surdar, Reduction potentials and exchange-reactions of thiyl radicals and disulfide radical anions, J.Phys.Chem., 1987, 91, 6532-6537.
    • 21. R.H.Bisby and N.Tabassum, Properties of the radicals formed by one-electron oxidation of acetaminophen, Biochem.Pharmacol., 1988, 37, 2731-2738.
    • 22. R.M.Williams, J.B.Shear, W.R.Zipfel, S.Maiti and W.W.Webb, Mucosal mast cell secretion processes imaged using three-photon microscopy of 5-hydroxytryptamine autofluorescence, Biophys.J., 1999, 76, 1835-1846.
    • 23. S.Maiti, J.B.Shear, R.M.Williams, W.R.Zipfel and W.W.Webb, Measuring serotonin distribution in live cells with three-photon excitation, Science, 1997, 275, 530-532.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article