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fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Clarke, Thomas Charles
Languages: English
Types: Doctoral thesis
Subjects: R1

Classified by OpenAIRE into

mesheuropmc: cardiovascular system, sense organs
In coronary heart disease, the blood supply to the myocardium is insufficient for its needs and leads to cardiac ischaemia, which is often accompanied by the onset of lethal cardiac arrhythmias. Electrical communication between cardiac myocytes occurs across gap junctions located in intercalated discs. Gap junctions are specialised structures of the plasma membrane which facilitate direct and rapid communication between adjacent cells. Connexin 43 (Cx43) is the most widely expressed of the connexin family and is the main connexin in the heart. A cardiac ischaemia model system was developed to study the various aspects of cardiac myocyte biology. Simulated ischaemia caused neonatal cardiac myocytes to cease synchronous contractions after 3-4 hours and was accompanied by the reversible dephosphorylation of Cx43 after 5 hours. Cx43 dephosphorylation was then reversed by reoxygenating the cells for 30 minutes. In hypoxia the myocytes continued to beat and Cx43 remained phosphorylated. Rotigaptide, an antiairhythmic peptide with protracted action, increased intercellular dye transfer in cardiac myocytes, atrial HL-1 cells and HeLa cells expressing Cx43. The communication-modifying effect of rotigaptide was confined to cells expressing Cx43 since the peptide had no effect on dye transfer in HeLa cells expressing Cx32 and Cx26. Rotigaptide had little effect on cell beating rate or Cx43 expression. Phosphorylation of Cx43 in normoxic and ischaemic cells was also unaffected. Simulated ischaemia induced cardiac myocytes to release a peak of ATP after 80 minutes that was blocked by the connexin mimetic peptide GAP 26 and the gap junction inhibitor 18-a glyccyrrhetinic acid. This suggested that the release of ATP occurred through connexin hemichannels (CxHcs). The rotigaptide analogue AAP10 increased the transient peak of ATP release caused by ischaemia. The model developed has provided a platform to study the effects of simulated ischaemia on Cx43 dependent functions in the heart. Rotigaptide and AAP10 emerge as novel peptides with therapeutic potential for treating heart arrhythmias.
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    • Gomes, P., Srinivas, S. P., Van, D. W., Vereecke, J., & Himpens, B. 2005, "ATP release through connexin hemichannels in corneal endothelial cells", Invest Ophthalmol. Vis.Sci., vol. 46, no. 4, pp. 1208-1218.
    • Gomez-Hemandez, J. M., de, M. M., Larrosa, B., Gonzalez, D., & Barrio, L. C. 2003, "Molecular basis of calcium regulation in connexin-32 hemichannels", Proc.Natl.Acad.Sci.U.S.A, vol. 100, no. 26, pp. 16030-16035.
    • Goodenough, D. A. & Paul, D. L. 2003, "Beyond the gap: functions o f unpaired connexon channels", Nat.Rev.Mol.CellBiol., vol. 4, no. 4, pp. 285-294.
    • Goodenough, D. A., Dick, J. S., & Lyons, J. E. 1980, "Lens metabolic cooperation: a study of mouse lens transport and permeability visualized with ffeeze-substitution autoradiography and electron microscopy", J.Cell Biol., vol. 86, no. 2, pp. 576-589.
    • Goodenough, D. A. & Revel, J. P. 1970, "A fine structural analysis o f intercellular junctions in the mouse liver", J. Cell Biol., vol. 45, no. 2, pp. 272-290.
    • 1993, "The spatial distribution and relative abundance of gap-junctional connexin40 and connexin43 correlate to functional properties o f components o f the cardiac atrioventricular conduction system", J.Cell Sci., vol. 105 ( Pt 4), pp. 985-991.
    • Gourdie, R. G., Green, C. R., & Severs, N. J. 1991, "Gap junction distribution in adult mammalian myocardium revealed by an anti-peptide antibody and laser scanning confocal microscopy", J.Cell Sci., vol. 99 ( Pt 1), pp. 41-55.
    • Green, C. R. & Severs, N. J. 1993, "Robert Feulgen Prize Lecture. Distribution and role of gap junctions in normal myocardium and human ischaemic heart disease", Histochemistry, vol. 99, no. 2, pp. 105-120.
    • Griffin, B. A., Adams, S. R., & Tsien, R. Y. 1998, "Specific covalent labeling of recombinant protein molecules inside live cells", Science, vol. 281, no. 5374, pp. 269- 272.
    • Gros, D., Jarry-Guichard, T., Ten, V., I, de, M. A., van Kempen, M. J., Davoust, J., Briand, J. P., Moorman, A. F., & Jongsma, H. J. 1994, "Restricted distribution of connexin40, a gap junctional protein, in mammalian heart", Circ.Res., vol. 74, no. 5, pp.
    • Grover, R. & Dhein, S. 2001, "Structure-activity relationships o f novel peptides related to the antiarrhythmic peptide AAP 10 which reduce the dispersion o f epicardial action potential duration", Peptides, vol. 22, no. 7, pp. 1011-1021.
    • Guerrero, P. A., Schuessler, R. B., Davis, L. M., Beyer, E. C., Johnson, C. M., Yamada, K. A., & Saffitz, J. E. 1997, "Slow ventricular conduction in mice heterozygous for a connexin43 null mutation", J.Clin.Invest, vol. 99, no. 8, pp. 1991-1998.
    • Gutstein, D. E., Morley, G. E., Tamaddon, H., Vaidya, D., Schneider, M. D., Chen, J., Chien, K. R., Stuhlmann, H., & Fishman, G. I. 2001, "Conduction slowing and sudden arrhythmic death in mice with cardiac-restricted inactivation o f connexin43", Circ.Res., vol. 88, no. 3, pp. 333-339.
    • Gutstein, D. E., Morley, G. E., Vaidya, D., Liu, F., Chen, F. L., Stuhlmann, H., & Fishman, G. I. 2001, "Heterogeneous expression o f Gap junction channels in the heart leads to conduction defects and ventricular dysfunction", Circulation, vol. 104, no. 10, pp. 1194-1199.
    • Haefliger, J. A. & Meda, P. 2000, "Chronic hypertension alters the expression o f Cx43 in cardiovascular muscle cells", Braz.J.Med.Biol.Res., vol. 33, no. 4, pp. 431-438.
    • Haissaguerre, M., Jais, P., Shah, D. C., Takahashi, A., Hocini, M., Quiniou, G., Garrigue, S., Le, M. A., Le, M. P., & Clementy, J. 1998, "Spontaneous initiation o f atrial fibrillation by ectopic beats originating in the pulmonary veins", N.Engl. J.Med., vol. 339, no. 10, pp. 659-666.
    • Halestrap, A. P., Clarke, S. J., & Javadov, S. A. 2004, "Mitochondrial permeability transition pore opening during myocardial reperfusion-a target for cardioprotection", Cardiovasc.Res., vol. 61, no. 3, pp. 372-385.
    • Hall, D. G., Morley, G. E., Vaidya, D., Ard, M., Kimball, T. R., Witt, S. A., & Colbert, M. C. 2000, "Early onset heart failure in transgenic mice with dilated cardiomyopathy", Pediatr.Res., vol. 48, no. 1, pp. 36-42.
    • Harris, A. L. 2001, "Emerging issues o f connexin channels: biophysics fills the gap", Q.Rev.Biophys., vol. 34, no. 3, pp. 325-472.
    • K., & Nielsen, M. S. 2005, "The antiarrhythmic peptide analog ZP123 prevents atrial conduction slowing during metabolic stress", J.Cardiovasc.Electrophysiol., vol. 16, no. 5, pp. 537-545.
    • Herman, J. K., Swillo, R. E., Morgan, G. A., Keith, J. C., Jr., Schaub, R. G., Smith, R. P., Feldman, H. S., Haugan, K., Kantrowitz, J., Wang, P. J., bu-Qare, A., Butera, J., Larsen, B. D., & Crandall, D. L. 2006, "Rotigaptide (ZP123) prevents spontaneous ventricular arrhythmias and reduces infarct size during myocardial ischemia/reperfusion injury in open-chest dogs", J.Pharmacol.Exp.Ther., vol. 317, no. 1, pp. 236-243.
    • Hernandez, J. & Ribeiro, J. A. 1996, "Excitatory actions o f adenosine on ventricular automaticity", Trends Pharmacol.Sci., vol. 17, no. 4, pp. 141-144.
    • Hernandez, O. M., Discher, D. J., Bishopric, N. H., & Webster, K. A. 2000, "Rapid activation of neutral sphingomyelinase by hypoxia-reoxygenation o f cardiac myocytes", Circ.Res., vol. 86, no. 2, pp. 198-204.
    • Heusch, G. 1998, "Hibernating myocardium", Physiol Rev., vol. 78, no. 4, pp. 1055-1085.
    • Hill, C. E., Rummery, N., Hickey, H., & Sandow, S. L. 2002, "Heterogeneity in the distribution o f vascular gap junctions and connexins: implications for function", Clin.Exp.Pharmacol.Physiol, vol. 29, no. 7, pp. 620-625.
    • Hirschi, K. K., Minnich, B. N., Moore, L. K., & Burt, J. M. 1993, "Oleic acid differentially affects gap junction-mediated communication in heart and vascular smooth muscle cells", AmJ.Physiol, vol. 265, no. 6 Pt 1, p. C1517-C1526.
    • Hoh, J. H., John, S. A., & Revel, J. P. 1991, "Molecular cloning and characterization o f a new member of the gap junction gene family, connexin-31", J.Biol.Chem., vol. 266, no.
    • 10, pp. 6524-6531.
    • Honjo, H., Boyett, M. R., Coppen, S. R., Takagishi, Y., Opthof, T., Severs, N. J., & Kodama, I. 2002, "Heterogeneous expression o f connexins in rabbit sinoatrial node cells: correlation between connexin isotype and cell size", Cardiovasc.Res., vol. 53, no. 1, pp.
    • Hu, X. & Dahl, G. 1999, "Exchange o f conductance and gating properties between gap junction hemichannels", FEBSLett., vol. 451, no. 2, pp. 113-117.
    • Hurtley, S. M. & Helenius, A. 1989, "Protein oligomerization in the endoplasmic reticulum", Annu.Rev.CellBiol., vol. 5, pp. 277-307.
    • Hwan, S. K. & Beyer, E. C. 2000, "Heterogeneous localization o f connexin40 in the renal vasculature", Microvasc.Res., vol. 59, no. 1, pp. 140-148.
    • Isakson, B. E., Evans, W. H., & Boitano, S. 2001, "Intercellular Ca2+ signaling in alveolar epithelial cells through gap junctions and by extracellular ATP", Am J.Physiol Lung Cell Mol.Physiol, vol. 280, no. 2, p. L221-L228.
    • John, S., Cesario, D., & Weiss, J. N. 2003, "Gap junctional hemichannels in the heart", Acta Physiol Scand., vol. 179, no. 1, pp. 23-31.
    • John, S. A., Kondo, R., Wang, S. Y., Goldhaber, J. I., & Weiss, J. N. 1999, "Connexin-43 hemichannels opened by metabolic inhibition", J.Biol.Chem., vol. 274, no. 1, pp. 236- 240.
    • Johnson, R. G., Meyer, R. A., Li, X. R., Preus, D. M., Tan, L., Grunenwald, H., Paulson, A. F., Laird, D. W., & Sheridan, J. D. 2002, "Gap junctions assemble in the presence o f cytoskeletal inhibitors, but enhanced assembly requires microtubules", Exp. Cell Res., vol.
    • 275, no. 1, pp. 67-80.
    • Jongsma, H. J. & Wilders, R. 2000, "Gap junctions in cardiovascular disease", Circ.Res., vol. 86, no. 12, pp. 1193-1197.
    • Jordan, K., Chodock, R., Hand, A. R., & Laird, D. W. 2001, "The origin o f annular junctions: a mechanism o f gap junction internalization", J Cell Sci., vol. 114, no. Pt 4, pp.
    • 1999, "Trafficking, assembly, and function o f a connexin43-green fluorescent protein chimera in live mammalian cells", Afol.Biol.Cell, vol. 10, no. 6, pp. 2033-2050.
    • Jung, F., Palmer, L. A., Zhou, N., & Johns, R. A. 2000, "Hypoxic regulation o f inducible nitric oxide synthase via hypoxia inducible factor-1 in cardiac myocytes", Circ.Res., vol.
    • 86, no. 3, pp. 319-325.
    • R , Yacoub, M. H., Rothery, S., & Severs, N. J. 2001, "Comparison o f connexin 43, 40 and 45 expression patterns in the developing human and mouse hearts", Cell Commun.Adhes., vol. 8, no. 4-6, pp. 339-343.
    • Kallio, P. J., Wilson, W. J., O'Brien, S., Makino, Y., & Poellinger, L. 1999, "Regulation of the hypoxia-inducible transcription factor 1alpha by the ubiquitin-proteasome pathway", J.Biol.Chem., vol. 274, no. 10, pp. 6519-6525.
    • Kanno, S., Kovacs, A., Yamada, K. A., & Saffitz, J. E. 2003, "Connexin43 as a determinant of myocardial infarct size following coronary occlusion in mice", J.Am.Coll.Cardiol., vol. 41, no. 4, pp. 681-686.
    • 1998, "Downregulation o f immunodetectable connexin43 and decreased gap junction size in the pathogenesis of chronic hibernation in the human left ventricle", Circulation, vol.
    • 97, no. 7, pp. 651-660.
    • Keeley, E. C., Boura, J. A., & Grines, C. L. 2003, "Primary angioplasty versus intravenous thrombolytic therapy for acute myocardial infarction: a quantitative review of 23 randomised trials", Lancet, vol. 361, no. 9351, pp. 13-20.
    • F., & Leigh, I. M. 1997, "Connexin 26 mutations in hereditary non-syndromic sensorineural deafness", Nature, vol. 387, no. 6628, pp. 80-83.
    • Kwak, B. R., Mulhaupt, F., Veillard, N., Gros, D. B., & Mach, F. 2002, "Altered pattern of vascular connexin expression in atherosclerotic plaques", Arterioscler.Thromb. Vasc.Biol., vol. 22, no. 2, pp. 225-230.
    • 1999, "Connexin expression in cultured neonatal rat myocytes reflects the pattern o f the intact ventricle", Cardiovasc.Res., vol. 44, no. 2, pp. 370-380.
    • Kwak, B. R. & Jongsma, H. J. 1996, "Regulation o f cardiac gap junction channel permeability and conductance by several phosphorylating conditions", Mol.Cell Biochem., vol. 157, no. 1-2, pp. 93-99.
    • Kwak, B. R., Saez, J. C., Wilders, R., Chanson, M., Fishman, G. I., Hertzberg, E. L., Spray, D. C., & Jongsma, H. J. 1995, "Effects o f cGMP-dependent phosphorylation on rat and human connexin43 gap junction channels", Pflugers Arch., vol. 430, no. 5, pp.
    • Kwak, B. R., van Veen, T. A., Analbers, L. J., & Jongsma, H. J. 1995, "TPA increases conductance but decreases permeability in neonatal rat cardiomyocyte gap junction channels", Exp.Cell Res., vol. 220, no. 2, pp. 456-463.
    • Laemmli, U. K. 1970, "Cleavage o f structural proteins during the assembly of the head of bacteriophage T4", Nature, vol. 227, no. 5259, pp. 680-685.
    • Lai, A., Le, D. N., Paznekas, W. A., Gifford, W. D., Jabs, E. W., & Charles, A. C. 2006, "Oculodentodigital dysplasia connexin43 mutations result in non-functional connexin hemichannels and gap junctions in C6 glioma cells", J.Cell Sci., vol. 119, no. Pt 3, pp.
    • Laing, J. G., Tadros, P. N., Westphale, E. M., & Beyer, E. C. 1997, "Degradation of connexin43 gap junctions involves both the proteasome and the lysosome", Exp. Cell Res., vol. 236, no. 2, pp. 482-492.
    • Laird, D. W. 2006, "Life cycle of connexins in health and disease", Biochem.J., vol. 394, no. Pt 3, pp. 527-543.
    • Laird, D. W. 2005, "Connexin phosphorylation as a regulatory event linked to gap junction internalization and degradation", Biochim.Biophys.Acta, vol. 1711, no. 2, pp.
    • Laird, D. W., Castillo, M., & Kasprzak, L. 1995, "Gap junction turnover, intracellular trafficking, and phosphorylation o f connexin43 in brefeldin A-treated rat mammary tumor cells", J.Cell Biol., vol. 131, no. 5, pp. 1193-1203.
    • Liang, G. S., de, M. M., Gomez-Hemandez, J. M., Glass, J. D., Scherer, S. S., Mintz, M., Barrio, L. C., & Fischbeck, K. H. 2005, "Severe neuropathy with leaky connexin32 hemichannels", Ann.Neurol, vol. 57, no. 5, pp. 749-754.
    • Lin, L. C., Wu, C. C., Yeh, H. I., Lu, L. S., Liu, Y. B., Lin, S. F., & Lee, Y. T. 2005, "Downregulated myocardial connexin 43 and suppressed contractility in rabbits subjected to a cholesterol-enriched diet", Lab Invest, vol. 85, no. 10, pp. 1224-1237.
    • Lin, R., Wam-Cramer, B. J., Kurata, W. E., & Lau, A. F. 2001, "v-Src phosphorylation o f connexin 43 on Tyr247 and Tyr265 disrupts gap junctional communication", J.Cell Biol., vol. 154, no. 4, pp. 815-827.
    • Little, T. L., Beyer, E. C., & Duling, B. R. 1995, "Connexin 43 and connexin 40 gap junctional proteins are present in arteriolar smooth muscle and endothelium in vivo", AmJ.Physiol, vol. 268, no. 2 Pt 2, p. H729-H739.
    • Litvin, O., Tiunova, A., Connell-Alberts, Y., Panchin, Y., & Baranova, A. 2006, "What is hidden in the pannexin treasure trove: the sneak peek and the guesswork", J.Cell Mol.Med., vol. 10, no. 3, pp. 613-634.
    • Locke, D., Liu, J., & Harris, A. L. 2005, "Lipid rafts prepared by different methods contain different connexin channels, but gap junctions are not lipid rafts", Biochemistry, vol. 44, no. 39, pp. 13027-13042.
    • Loddenkemper, T., Grote, K., Evers, S., Oelerich, M., & Stogbauer, F. 2002, "Neurological manifestations o f the oculodentodigital dysplasia syndrome", J.Neurol., vol. 249, no. 5, pp. 584-595.
    • Loewenstein, W. R. 1981, "Junctional intercellular communication: the cell-to-cell membrane channel", Physiol Rev., vol. 61, no. 4, pp. 829-913.
    • Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. 1951, "Protein measurement with the Folin phenol reagent", J.Biol.Chem., vol. 193, no. 1, pp. 265-275.
    • Luke, R. A. & Saffitz, J. E. 1991, "Remodeling o f ventricular conduction pathways in healed canine infarct border zones", J.Clin.Invest, vol. 87, no. 5, pp. 1594-1602.
    • Makowski, L., Caspar, D. L., Phillips, W. C., & Goodenough, D. A. 1977, "Gap junction structures. II. Analysis of the x-ray diffraction data", J.Cell Biol., vol. 74, no. 2, pp. 629- 645.
    • Malchow, R. P., Qian, H., & Ripps, H. 1993, "Evidence for hemi-gap junctional channels in isolated horizontal cells o f the skate retina", J.Neurosci.Res., vol. 35, no. 3, pp. 237- 245.
    • Martin, P. E. & Evans, W. H. 2004, "Incorporation of connexins into plasma membranes and gap junctions", Cardiovasc.Res., vol. 62, no. 2, pp. 378-387.
    • Martin, P. E., Hill, N. S., Kristensen, B., Errington, R. J., & Rachael, J. T. 2004, "Ouabain exerts biphasic effects on connexin functionality and expression in vascular smooth muscle cells", Br.J.Pharmacol., vol. 141, no. 2, pp. 374-384.
    • Martin, P. E., Blundell, G., Ahmad, S., Errington, R. J., & Evans, W. H. 2001, "Multiple pathways in the trafficking and assembly o f connexin 26, 32 and 43 into gap junction intercellular communication channels", J.Cell Sci., vol. 114, no. Pt 21, pp. 3845-3855.
    • Martin, P. E., Errington, R. J., & Evans, W. H. 2001, "Gap junction assembly: multiple connexin fluorophores identify complex trafficking pathways", Cell Commun.Adhes., vol.
    • 8, no. 4-6, pp. 243-248.
    • Martin, P. E., Mambetisaeva, E. T., Archer, D. A., George, C. H., & Evans, W. H. 2000, "Analysis of gap junction assembly using mutated connexins detected in Charcot-MarieTooth X-linked disease", J.Neurochem., vol. 74, no. 2, pp. 711-720.
    • Martin, P. E., Steggles, J., Wilson, C., Ahmad, S., & Evans, W. H. 2000, "Targeting motifs and functional parameters governing the assembly o f connexins into gap junctions", Biochem.J., vol. 349, no. Pt 1, pp. 281-287.
    • Martin, P. E., Coleman, S. L., Casalotti, S. O., Forge, A., & Evans, W. H. 1999, "Properties of connexin26 gap junctional proteins derived from mutations associated with non-syndromal heriditary deafness", Hum.Mol.Genet., vol. 8, no. 13, pp. 2369-2376.
    • Martin, P. E., George, C. H., Castro, C., Kendall, J. M., Capel, J., Campbell, A. K., Revilla, A., Barrio, L. C., & Evans, W. H. 1998, "Assembly of chimeric connexinaequorin proteins into functional gap junction channels. Reporting intracellular and plasma membrane calcium environments", J.Biol.Chem., vol. 273, no. 3, pp. 1719-1726.
    • Milks, L. C., Kumar, N. M., Houghten, R., Unwin, N., & Gilula, N. B. 1988, "Topology of the 32-kd liver gap junction protein determined by site-directed antibody localizations", EMBOJ., vol. 7, no. 10, pp. 2967-2975.
    • Mizuguchi, H. & Kay, M. A. 1998, "Efficient construction o f a recombinant adenovirus vector by an improved in vitro ligation method", Hum.Gene Ther., vol. 9, no. 17, pp.
    • Moreno, A. P. 2005, "Connexin phosphorylation as a regulatory event linked to channel gating", Biochim.Biophys.Acta, vol. 1711, no. 2, pp. 164-171.
    • Reaume, A. G., De Sousa, P. A., Kulkami, S., Langille, B. L., Zhu, D., Davies, T. C., Juneja, S. C., Kidder, G. M., & Rossant, J. 1995, "Cardiac malformation in neonatal mice lacking connexin43", Science, vol. 267, no. 5205, pp. 1831-1834.
    • C. 1993, "Molecular cloning and functional expression of human connexin37, an endothelial cell gap junction protein", J.Clin.Invest, vol. 91, no. 3, pp. 997-1004.
    • Retamal, M. A., Cortes, C. J., Reuss, L., Bennett, M. V., & Saez, J. C. 2006, "Snitrosylation and permeation through connexin 43 hemichannels in astrocytes: induction by oxidant stress and reversal by reducing agents", Proc.Natl.Acad.Sci. U.S.A, vol. 103, no. 12, pp. 4475-4480.
    • Revel, J. P. & Kamovsky, M. J. 1967, "Hexagonal array o f subunits in intercellular junctions of the mouse heart and liver", J.Cell Biol., vol. 33, no. 3, p. C7-C12.
    • Reynhout, J. K., Lampe, P. D., & Johnson, R. G. 1992, "An activator o f protein kinase C inhibits gap junction communication between cultured bovine lens cells", Exp.Cell Res., vol. 198, no. 2, pp. 337-342.
    • Ripps, H., Qian, H., & Zakevicius, J. 2004, "Properties o f connexin26 hemichannels expressed in Xenopus oocytes", Cell Mol.Neurobiol., vol. 24, no. 5, pp. 647-665.
    • Rivard, A., Berthou-Soulie, L., Principe, N., Kearney, M., Curry, C., Branellec, D., Semenza, G. L., & Isner, J. M. 2000, "Age-dependent defect in vascular endothelial growth factor expression is associated with reduced hypoxia-inducible factor 1 activity", J.Biol.Chem., vol. 275, no. 38, pp. 29643-29647.
    • Rivedal, E., Mollerup, S., Haugen, A., & Vikhamar, G. 1996, "Modulation o f gap junctional intercellular communication by EGF in human kidney epithelial cells", Carcinogenesis, vol. 17, no. 11, pp. 2321-2328.
    • B., & Erlich, H. A. 1988, "Primer-directed enzymatic amplification o f DNA with a thermostable DNA polymerase", Science, vol. 239, no. 4839, pp. 487-491.
    • Spray, D. C., Ye, Z. C., & Ransom, B. R. 2006, "Functional connexin "hemichannels": A critical appraisal", Glia, vol. 54, no. 7, pp. 758-773.
    • Spray, D. C. & Burt, J. M. 1990, "Structure-activity relations o f the cardiac gap junction channel", Am.J.Physiol, vol. 258, no. 2 Pt 1, p. C195-C205.
    • Srinivas, M., Calderon, D. P., Kronengold, J., & Verselis, V. K. 2006, "Regulation o f connexin hemichannels by monovalent cations", J.Gen.Physiol, vol. 127, no. 1, pp. 67- 75.
    • Srinivas, M., Kronengold, J., Bukauskas, F. F., Bargiello, T. A., & Verselis, V. K. 2005, "Correlative studies o f gating in Cx46 and Cx50 hemichannels and gap junction channels", Biophys.J., vol. 88, no. 3, pp. 1725-1739.
    • Starich, T., Sheehan, M., Jadrich, J., & Shaw, J. 2001, "Innexins in C. elegans", Cell Commun.Adhes., vol. 8, no. 4-6, pp. 311-314.
    • Stebbings, L. A., Todman, M. G., Phillips, R., Greer, C. E., Tam, J., Phelan, P., Jacobs, K., Bacon, J. P., & Davies, J. A. 2002, "Gap junctions in Drosophila: developmental expression of the entire innexin gene family", Mech.Dev., vol. 113, no. 2, pp. 197-205.
    • Steel, K. P. & Kros, C. J. 2001, "A genetic approach to understanding auditory function", Nat.Genet., vol. 27, no. 2, pp. 143-149.
    • Stewart, W. W. 1981, "Lucifer dyes-highly fluorescent dyes for biological tracing", Nature, vol. 292, no. 5818, pp. 17-21.
    • Stout, C., Goodenough, D. A., & Paul, D. L. 2004, "Connexins: functions without junctions", Curr.Opin.CellBiol., vol. 16, no. 5, pp. 507-512.
    • Stout, C. E., Costantin, J. L., Naus, C. C., & Charles, A. C. 2002, "Intercellular calcium signaling in astrocytes via ATP release through connexin hemichannels", J.Biol.Chem., vol. 277, no. 12, pp. 10482-10488.
    • Suchyna, T. M., Xu, L. X., Gao, F., Fourtner, C. R., & Nicholson, B. J. 1993, "Identification of a proline residue as a transduction element involved in voltage gating of gap junctions", Nature, vol. 365, no. 6449, pp. 847-849.
    • White, T. W., Deans, M. R., Kelsell, D. P., & Paul, D. L. 1998, "Connexin mutations in deafness", Nature, vol. 394, no. 6694, pp. 630-631.
    • Wijffels, M. C., Kirchhof, C. J., Dorland, R., & Allessie, M. A. 1995, "Atrial fibrillation begets atrial fibrillation. A study in awake chronically instrumented goats", Circulation, vol. 92, no. 7, pp. 1954-1968.
    • Willecke, K., Eiberger, J., Degen, J., Eckardt, D., Romualdi, A., Guldenagel, M., Deutsch, U., & Sohl, G. 2002, "Structural and functional diversity of connexin genes in the mouse and human genome", Biol.Chem., vol. 383, no. 5, pp. 725-737.
    • Yin, X., Gu, S., & Jiang, J. X. 2001, "The development-associated cleavage o f lens connexin 45.6 by caspase-3-like protease is regulated by casein kinase II-mediated phosphorylation", J.Biol.Chem., vol. 276, no. 37, pp. 34567-34572.
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