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Kirk Ruth S; Davies Angela J; Zahoor Zahida; Rollinson David; Walker Anthony J (2009)
Publisher: BioMed Central
Journal: Parasites & Vectors
Languages: English
Types: Article
Subjects: RC109-216, Infectious and parasitic diseases, biological, Research

Classified by OpenAIRE into

mesheuropmc: parasitic diseases, biochemical phenomena, metabolism, and nutrition

Abstract

Background

Schistosoma mansoni uses Biomphalaria glabrata as an intermediate host during its complex life cycle. In the snail, the parasite initially transforms from a miracidium into a mother sporocyst and during this process excretory-secretory products (ESPs) are released. Nitric oxide (NO) and its reactive intermediates play an important role in host defence responses against pathogens. This study therefore aimed to determine the effects of S. mansoni ESPs on NO production in defence cells (haemocytes) from schistosome-susceptible and schistosome-resistant B. glabrata strains. As S. mansoni ESPs have previously been shown to inhibit extracellular signal-regulated kinase (ERK) phosphorylation (activation) in haemocytes from susceptible, but not resistant, B. glabrata the regulation of NO output by ERK in these cells was also investigated.

Results

Haemocytes from resistant snails challenged with S. mansoni ESPs (20 μg/ml) over 5 h displayed an increase in NO production that was 3.3 times greater than that observed for unchallenged haemocytes; lower concentrations of ESPs (0.1–10 μg/ml) did not significantly increase NO output. In contrast, haemocytes from susceptible snails showed no significant change in NO output following challenge with ESPs at any concentration used (0.1–20 μg/ml). Western blotting revealed that U0126 (1 μM or 10 μM) blocked the phosphorylation (activation) status of ERK in haemocytes from both snail strains. Inhibition of ERK signalling by U0126 attenuated considerably intracellular NO production in haemocytes from both susceptible and resistant B. glabrata strains, identifying ERK as a key regulator of NO output in these cells.

Conclusion

S. mansoni ESPs differentially influence intracellular NO levels in susceptible and resistant B. glabrata haemocytes, possibly through modulation of the ERK signalling pathway. Such effects might facilitate survival of S. mansoni in its intermediate host.

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    • 1. Raines KW, Bonini MG, Campbell SL: Nitric oxide cell signaling: S-nitrosation of ras superfamily GTPases. Cardiovasc Res 2007, 75:229-239.
    • 2. Kleinert H, Pautz A, Linkker K, Schwarz MP: Regulation of the expression of inducible nitric oxide synthase. Euro J Pharmacol 2004, 500:255-266.
    • 3. Rivero A: Nitric oxide: An antiparasitic molecule of invertebrates. Trends Parasitol 2006, 22:219-225.
    • 4. Matsuo R, Misawa K, Ito E: Genomic structure of nitric oxide synthase in the terrestrial slug is highly conserved. Gene 2008, 415:74-81.
    • 5. Wright B, Lacchini AH, Davies AJ, Walker AJ: Regulation of nitric oxide production in snail (Lymnaea stagnalis) defence cells: A role for PKC and ERK signalling pathways. Biol Cell 2006, 98:265-278.
    • 6. Novas A, Cao A, Barcia R, Ramos-Martinez JI: Nitric oxide release by hemocytes of the mussel Mytilus galloprovincialis Lmk was provoked by interleukin-2 but not by lipopolysaccharide. Int J Biochem Cell Biol 2004, 36:390-394.
    • 7. Hahn UK, Bender RC, Bayne CJ: Involvement of nitric oxide in killing of Schistosoma mansoni sporocysts by hemocytes from resistant Biomphalaria glabrata. J Parasitol 2001, 187:778-785.
    • 8. Korneev SA, Piper MR, Picot J, Phillips R, Korneeva EI, O'Shea M: Molecular characterization of NOS in a mollusc: Expression in a giant modulatory neuron. J Neurobiol 1998, 35:65-76.
    • 9. Bodnarova M, Martasek P, Moroz LL: Calcium/calmodulindependent nitric oxide synthase activity in the CNS of Aplysia californica : Biochemical characterization and link to cGMP pathways. J Inorg Biochem 2005, 99:922-928.
    • 10. Moroz L, Winlow W, Turner RW, Bulloch AG, Lukowiak K, Syed NI: Nitric oxide synthase-immunoreactive cells in the CNS and periphery of Lymnaea. Neuroreport 1994, 5:1277-1280.
    • 11. Elofsson R, Carlberg M, Moroz L, Nezlin L, Sakharov D: Is nitric oxide (NO) produced by invertebrate neurones? Neuroreport 1993, 4:279-282.
    • 12. Bayne CJ: Successful parasitism of vector snail Biomphalaria glabrata by the human blood fluke (trematode) Schistosoma mansoni: a 2009 assessment. Mol Biochem Parasitol 2009, 165:8-18.
    • 13. Humphries JE, Yoshino TP: Cellular receptors and signal transduction in molluscan hemocytes: Connections with the innate immune system of vertebrates. Integr Comp Biol 2003, 43:305-312.
    • 14. Yoshino TP, Boyle JP, Humphries JE: Receptor-ligand interactions and cellular signalling at the host-parasite interface. Parasitology 2001, 123 Suppl:S143-S157.
    • 15. Lacchini AH, Davies AJ, Mackintosh D, Walker AJ: Beta-1, 3-glucan modulates PKC signalling in Lymnaea stagnalis defence cells: A role for PKC in H2O2 production and downstream ERK activation. J Exp Biol 2006, 209:4829-4840.
    • 16. Zahoor Z, Davies AJ, Kirk RS, Rollinson D, Walker AJ: Disruption of ERK signalling in Biomphalaria glabrata defence cells by Schistosoma mansoni: Implications for parasite survival in the snail host. Dev Comp Immunol 2008, 32:1561-1571.
    • 17. Walker AJ, Rollinson D: Specific tyrosine phosphorylation induced in Schistosoma mansoni miracidia by haemolymph from schistosome susceptible, but not resistant, Biomphalaria glabrata. Parasitology 2008, 135:337-345.
    • 18. Plows LD, Cook RT, Davies AJ, Walker AJ: Activation of extracellular signal-regulated kinase is required for phagocytosis by Lymnaea stagnalis haemocytes. Biochim Biophys Acta 2004, 1692:25-33.
    • 19. Collmann C, Carlsson MA, Hansson BS, Nighorn A: Odorantevoked nitric oxide signals in the antennal lobe of Manduca sexta. J Neurosci 2004, 24:6070-6077.
    • 20. Davidson SK, Koropatnick TA, Kossmehl R, Sycuro L, McFall-Ngai MJ: NO means 'yes' in the squid-vibrio symbiosis: Nitric oxide (NO) during the initial stages of a beneficial association. Cell Microbiol 2004, 6:1139-1151.
    • 21. Comes S, Locascio A, Silvestre F, d'Ischia M, Russo GL, Tosti E, Branno M, Palumbo A: Regulatory roles of nitric oxide during larval development and metamorphosis in Ciona intestinalis. Dev Biol 2007, 306:772-784.
    • 22. Franchini A, Conte A, Ottaviani E: Nitric oxide: An ancestral immunocyte effector molecule. Adv Neuroimmunol 1995, 5:463-478.
    • 23. Arumugam M, Romestand B, Torreilles J, Roch P: In vitro production of superoxide and nitric oxide (as nitrite and nitrate) by Mytilus galloprovincialis haemocytes upon incubation with PMA or laminarin or during yeast phagocytosis. Eur J Cell Biol 2000, 79:513-519.
    • 24. Tafalla C, Gomez-Leon J, Novoa B, Figueras A: Nitric oxide production by carpet shell clam (Ruditapes decussatus) hemocytes. Dev Comp Immunol 2003, 27:197-205.
    • 25. Hahn UK, Bender RC, Bayne CJ: Killing of Schistosoma mansoni sporocysts by hemocytes from resistant Biomphalaria glabrata: Role of reactive oxygen species. J Parasitol 2001, 87:292-299.
    • 26. Hahn UK, Bender RC, Bayne CJ: Production of reactive oxygen species by hemocytes of Biomphalaria glabrata : Carbohydrate-specific stimulation. Dev Comp Immunol 2000, 24:531-541.
    • 27. Bayne CJ: Phagocytosis and non-self recognition in invertebrates. Bioscience 1990, 40:723-731.
    • 28. Connors VA, Yoshino TP: In vitro effect of larval Schistosoma mansoni excretory-secretory products on phagocytosis-stimulated superoxide production in hemocytes from Biomphalaria glabrata. J Parasitol 1990, 76:895-902.
    • 29. Lodes MJ, Yoshino TP: The effect of schistosome excretorysecretory products on Biomphalaria glabrata hemocyte motility. J Invert Pathol 1990, 56:75-85.
    • 30. Connors VA, Lodes MJ, Yoshino TP: Identification of a Schistosoma mansoni sporocyst excretory-secretory antioxidant molecule and its effect on superoxide production by Biomphalaria glabrata hemocytes. J Invert Pathol 1991, 58:387-395.
    • 31. Bender RC, Broderick EJ, Goodall CP, Bayne CJ: Respiratory burst of Biomphalaria glabrata hemocytes: Schistosoma mansoniresistant snails produce more extracellular H2O2 than susceptible snails. J Parasitol 2005, 91:275-279.
    • 32. Humphries JE, Yoshino TP: Regulation of hydrogen peroxide release in circulating hemocytes of the planorbid snail Biomphalaria glabrata. Dev Comp Immunol 2008, 32:554-562.
    • 33. Zelck UE, Gege BE, Schmid S: Specific inhibitors of mitogen-activated protein kinase and PI3-K pathways impair immune responses by hemocytes of trematode intermediate host snails. Dev Comp Immunol 2007, 31:321-331.
    • 34. Lüder CG, Algner M, Lang C, Bleicher N, Gross U: Reduced expression of the inducible nitric oxide synthase after infection with Toxoplasma gondii facilitates parasite replication in activated murine macrophages. Int J Parasitol 2003, 33:833-844.
    • 35. Becker K, Tilley L, Vennerstrom JL, Roberts D, Rogerson S, Ginsburg H: Oxidative stress in malaria parasite-infected erythrocytes: Host-parasite interactions. Int J Parasitol 2004, 34:163-189.
    • 36. Nagy G, Clark JM, Buzas EI, Gorman CL, Cope AP: Nitric oxide, chronic inflammation and autoimmunity. Immunol Lett 2007, 111:1-5.
    • 37. Veal EA, Day AM, Morgan BA: Hydrogen peroxide sensing and signaling. Mol Cell 2007, 26:1-14.
    • 38. Sminia T: Structure and function of blood and connective tissue cells of the fresh water pulmonate Lymnaea stagnalis studied by electron microscopy and enzyme histochemistry. Z Zellforsch Mikrosk Anat 1972, 130:497-526.
    • 39. Plows LD, Cook RT, Davies AJ, Walker AJ: Carbohydrates that mimic schistosome surface coat components affect ERK and PKC signalling in Lymnaea stagnalis haemocytes. Int J Parasitol 2005, 35:293-302.
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