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Lei, Yuan; Garrahan, Nigel John; Hermann, Boris; Becker, David L.; Hernandez, M. Rosario; Boulton, Michael E.; Morgan, James Edwards (2008)
Publisher: Association for Research in Vision and Ophthalmology
Languages: English
Types: Article
Subjects: RE

Classified by OpenAIRE into

mesheuropmc: sense organs, genetic structures, eye diseases
purpose. Glaucoma is presumed to result in the selective loss of retinal ganglion cells. In many neural systems, this loss would initiate a cascade of transneuronal degeneration. The quantification of changes in neuronal populations in the middle layers of the retina can be difficult with conventional histologic techniques. A method was developed based on multiphoton imaging of 4′,6′-diamino-2-phenylindole (DAPI)–stained tissue to quantify neuron loss in postmortem human glaucomatous retinas. methods. Retinas from normal and glaucomatous eyes fixed in 4% paraformaldehyde were incubated at 4°C overnight in DAPI solution. DAPI-labeled neurons at different levels of the retina were imaged by multiphoton confocal microscopy. Algorithms were developed for the automated identification of neurons in the retinal ganglion cell layer (RGCL), inner nucleus layer (INL), and outer nuclear layer (ONL). results. In glaucomatous retinas, the mean density of RGCs within 4 mm eccentricity was reduced by approximately 45%, with the greatest RGC loss occurring in a region that corresponds to the central 6° to 14° of vision. Significant neuron loss in the INL and ONL was also seen at 2 to 4 mm and 2 to 3 mm eccentricities, respectively. The ratios of neuron densities in the INL and ONL relative to the RGCL (INL/RGC and ONL/RGC, respectively) were found to increase significantly at 3 to 4 mm eccentricity. conclusions. The data confirm that the greatest neuronal loss occurs in the RGCL in human glaucoma. Neuronal loss was also observed in the outer retinal layers (INL and ONL) that correlated spatially with changes in the RGCL. Further work is necessary to confirm whether these changes arise from transneuronal degeneration.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1. Janssen P, Naskar R, Moore S, Thanos S, Thiel H. Evidence for glaucoma-induced horizontal cell alterations in the human retina. Ger J Ophthalmol. 1996;5:378 -385.
    • 2. May CA, Mittag T. Neuronal nitric oxide synthase (nNOS) positive retinal amacrine cells are altered in the DBA/2NNia mouse, a murine model for angle-closure glaucoma. J Glaucoma. 2004;13: 496 - 499.
    • 3. Moon JI, Kim IB, Gwon JS, et al. Changes in retinal neuronal populations in the DBA/2J mouse. Cell Tissue Res. 2005;320:51- 59.
    • 4. Wang X, Ng YK, Tay SS. Factors contributing to neuronal degeneration in retinas of experimental glaucomatous rats. J Neurosci Res. 2005;82:674 - 689.
    • 5. Dkhissi O, Chanut E, Versaux-Botteri C, et al. Changes in retinal dopaminergic cells and dopamine rhythmic metabolism during the development of a glaucoma-like disorder in quails. Invest Ophthalmol Vis Sci. 1996;37:2335-2344.
    • 6. Osborne NN, Ugarte M, Chao M, et al. Neuroprotection in relation to retinal ischemia and relevance to glaucoma. Surv Ophthalmol. 1999;43(suppl 1):S102-S128.
    • 7. Nork TM, Ver Hoeve JN, Poulsen GL, et al. Swelling and loss of photoreceptors in chronic human and experimental glaucomas. Arch Ophthalmol. 2000;118:235-245.
    • 8. Panda S, Jonas JB. Decreased photoreceptor count in human eyes with secondary angle-closure glaucoma. Invest Ophthalmol Vis Sci. 1992;33:2532-2536.
    • 9. Nork TM. Acquired color vision loss and a possible mechanism of ganglion cell death in glaucoma. Trans Am Ophthalmol Soc. 2000; 98:331-363.
    • 10. Raz D, Perlman I, Percicot CL, Lambrou GN, Ofri R. Functional damage to inner and outer retinal cells in experimental glaucoma. Invest Ophthalmol Vis Sci. 2003;44:3675-3684.
    • 11. Bayer AU, Neuhardt T, May AC, et al. Retinal morphology and ERG response in the DBA/2NNia mouse model of angle-closure glaucoma. Invest Ophthalmol Vis Sci. 2001;42:1258 -1265.
    • 12. Curcio CA, Allen KA. Topography of ganglion cells in human retina. J Comp Neurol. 1990;300:5-25.
    • 13. Curcio CA, Drucker DN. Retinal ganglion cells in Alzheimer's disease and aging. Ann Neurol. 1993;33:248 -257.
    • 14. Tauer U. Advantages and risks of multiphoton microscopy in physiology. Exp Physiol. 2002;87:709 -714.
    • 15. Kerrigan-Baumrind LA, Quigley HA, Pease ME, Kerrigan DF, Mitchell RS. Number of ganglion cells in glaucoma eyes compared with threshold visual field tests in the same persons. Invest Ophthalmol Vis Sci. 2000;41:741-748.
    • 16. Morgan JE, Uchida H, Caprioli J. Retinal ganglion cell death in experimental glaucoma. Br J Ophthalmol. 2000;84:303-310.
    • 17. Drasdo N, Fowler CW. Non-linear projection of the retinal image in a wide-angle schematic eye. Br J Ophthalmol. 1974;58:709 -714.
    • 18. Byun J, Verardo MR, Sumengen B, et al. Automated tool for the detection of cell nuclei in digital microscopic images: application to retinal images. Mol Vis. 2006;12:949 -960.
    • 19. Rasband WS. ImageJ, U. S. National Institutes of Health, Bethesda, MD; 1997-2006. Available at http://rsb.info.nih.gov/ij/.
    • 20. Curcio CA. Photoreceptor topography in ageing and age-related maculopathy. Eye. 2001;15:376 -383.
    • 21. Pelzel HR, Schlamp CL, Poulsen GL, et al. Decrease of cone opsin mRNA in experimental ocular hypertension. Mol Vis. 2006;12: 1272-1282.
    • 22. Honkanen RA, Baruah S, Zimmerman MB, et al. Vitreous amino acid concentrations in patients with glaucoma undergoing vitrectomy. Arch Ophthalmol. 2003;121:183-188.
    • 23. Carter-Dawson L, Shen FF, Harwerth RS, et al. Glutathione content is altered in Muller cells of monkey eyes with experimental glaucoma. Neurosci Lett. 2004;364:7-10.
    • 24. Hartwick AT, Zhang X, Chauhan BC, Baldridge WH. Functional assessment of glutamate clearance mechanisms in a chronic rat glaucoma model using retinal ganglion cell calcium imaging. J Neurochem. 2005;94:794 - 807.
    • 25. Lipton SA. The molecular basis of memantine action in Alzheimer's disease and other neurologic disorders: low-affinity, uncompetitive antagonism. Curr Alzheimer Res. 2005;2:155-165.
    • 26. Lin MT, Beal MF. Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature. 2006;443:787-795.
    • 27. Levin LA. Relevance of the site of injury of glaucoma to neuroprotective strategies. Surv Ophthalmol. 2001;45(suppl 3):S243-S249; discussion S273-S246.
    • 28. Nickells RW. From ocular hypertension to ganglion cell death: a theoretical sequence of events leading to glaucoma. Can J Ophthalmol. 2007;42:278 -287.
    • 29. Levkovitch-Verbin H, Quigley HA, Kerrigan-Baumrind LA, et al. Optic nerve transection in monkeys may result in secondary degeneration of retinal ganglion cells. Invest Ophthalmol Vis Sci. 2001;42:975-982.
    • 30. Levkovitch-Verbin H, Quigley HA, Martin KRG, et al. A model to study differences between primary and secondary degeneration of retinal ganglion cells in rats by partial optic nerve transection. Invest Ophthalmol Vis Sci. 2003;44:3388 -3393.
    • 31. Drasdo N, Aldebasi YH, Chiti Z, et al. The s-cone PHNR and pattern ERG in primary open angle glaucoma. Invest Ophthalmol Vis Sci. 2001;42:1266 -1272.
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