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fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Languages: English
Types: Doctoral thesis
Subjects: UOWSAT
Diabetic foot ulcer (DFU), a major complication of both types 1 and 2 diabetes, develops in about 15–25% of people living with the disease. In Ghana, DFUs contribute to most hospital admissions (53%) among diabetics with high rates of amputation (33.3%) and death (8.8%). Diabetic foot ulcers are predisposed to infections from bacteria in the environment which normally colonise these wounds as multicellular communities called biofilms. Biofilms have been found to have increased resistance to antimicrobial agents probably due to the presence of an extracellular matrix that retards or prevents the entry of antimicrobial agents into the bacterial community, antibiotic resistance genes and/or the presence of persister cells that are unresponsive to antimicrobial agents. The work presented here studied the role of 2 multidrug resistant DFU isolates, Klebsiella pneumoniae and Proteus mirabilis in maintaining the chronicity of diabetic foot ulcers. Using 3 in vitro biofilm models; the conventional microtitre plate and Minimum Biofilm Eradication Concentration (MBEC™) High-Throughput assays and the Quasi–Vivo® continuous flow system, K. pneumoniae and P. mirabilis were found to be positive for acyl–homoserine lactone production, biofilm and persister cell producers and could resist and/or tolerate antibiotics such as ceftazidime and levofloxacin up to 1280 times their minimum inhibitory concentration. K. pneumoniae and P. mirabilis were also found to express the interspecies AI–2 quorum sensing molecules which significantly increased biofilm formation and fold induction of bioluminescence in a luxS mutant V. harveyi reference strain. Quorum sensing (QS) inhibition assays using baicalin hydrate, cinnamaldehyde and 2(5H)–furanone showed considerable inhibition of K. pneumoniae and P. mirabilis biofilm formation but failed to completely inhibit their growth. The combinatorial effects of antibiotics and QS inhibitors/antimicrobial peptides such as polymyxin B and polymyxin B nonapeptide determined as fractional inhibitory concentration (FIC) index suggests that, additive and synergistic effects produced by the combination of two antimicrobial agents have the potential to eradicate biofilms. Data from the FIC indices determined from the combination assays can provide the basis for the formulation of topical treatment for DFUs.
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    • tumefaciens NCIMB 14543 biosensor reporter strain. ........................................................181 Figure 5.11 AI-2 cross-stimulation assay for light production in V. harveyi. .....................182 Figure 5.12 Fold induction of luminescence of V. harveyi NCIMB 1280 reporter strain. ...186 Figure 5.13 Biofilm formation by V. harveyi NCIMB 1280 reference strain before and after mutagenesis and addition of exogenous AI-2. ......................................................................187 Figure 5.14 Specificity of carbohydrate-binding lectin to polysaccharides on whole cells of K. pneumoniae (ODKp), P. mirabilis (ODPm) and S. aureus (ODSa).. ....................................188 Figure 5.15 Effect of wound dressings on biofilm formation by K. pneumoniae and P.
    • mirabilis.. ...............................................................................................................................197 Figure 5.16 Combined effects of wound dressings and antibiotics ......................................198 Figure 5.17 Biofilm phenotypes of P. mirabilis in the presence of ......................................202 Figure 5.18 Combined effects of wound dressings and antibiotics. .....................................203 Figure 5.19 Combined effects of wound dressings and antibiotics. .....................................205 Gyamfi-Brobbey, G., Greenwell, P., Kimmitt, P. (2012). Diabetic foot ulcers in Ghana.
    • Poster presentation at the 2012 Postgraduate Fair, University of Westminster, London, UK Gyamfi-Brobbey, G., Greenwell, P., Kimmitt, P. (2013). Diabetic foot ulcers: prevalence of diabetic foot infections among the diabetic population attending the Komfo Anokye Teaching Hospital, Kumasi, Ghana. Poster presentation at the 2013 Postgraduate Fair, University of Westminster, London, UK Gyamfi-Brobbey, G., Greenwell, P., Kimmitt, P. (2014). The microbiology of diabetic foot ulcers: A Ghanaian perspective. Poster presentation at the 2014 Postgraduate Fair, University of Westminster, London, UK Gyamfi-Brobbey, G., Greenwell, P., Kimmitt, P. (2015). The microbiology of diabetic foot ulcers: A Ghanaian perspective. Oral and poster presentation at the 2015 Postgraduate Fair and 2015 Annual Graduate School Reception - dubbed “Next Generation Research,” University of Westminster, London, UK Omotosho, O., Gyamfi-Brobbey, G., Greenwell, P., Kimmitt, P. (2015). In vitro study of the effect of wound dressings on planktonic cells and biofilms from diabetic foot infections.
    • Poster presentation at the Society for General Microbiology Annual Conference, Birmingham, UK Gyamfi-Brobbey, G., Greenwell, P., Kimmitt, P. (2015). In vitro assessment of the synergistic effects of antibiotics and wound dressings on biofilms from diabetic foot pathogens. Poster presentation at the Society for General Microbiology Annual Conference, Birmingham, UK Gyamfi-Brobbey, G., Greenwell, P., Kimmitt, P. (2015). Cinnamaldehyde increases the susceptibility of quorum-sensing-mediated biofilms to conventional antibiotics. Poster presentation at the Society for General Microbiology Annual Conference, Birmingham, UK Johnson, J. D. and Luciani, D. S. (2010). Mechanisms of pancreatic beta-cell apoptosis in diabetes and its therapies. AdvExp Med Biol. 654, 447-62.
    • Jones, E. M., Cochrane, C. A., Percival, S. L. (2015). The effect of pH on the extracellular matrix and biofilms. Advances in Wound Care, 4(7), 431-439.
    • Jude, E. B., Boulton, A. J. M. (1999). End stage complications of diabetic neuropathy.
    • Diabetes Rev. 7:395- 410.
    • Juhas, M., Eberl, L., Tümmler, B. (2005). Quorum sensing: the power of cooperation in the world of Pseudomonas. Environmental Microbiology, 7(4), 459-471.
    • Kamilar, J. M., Cooper, N. (2013). Phylogenetic signal in primate behaviour, ecology and life history. Philosophical Transactions of the Royal Society B: Biological Sciences, 368(1618), 20120341. Available from [Accessed 21/12/2015] Kamiya, H., Ehara, T., Matsumoto, T. (2012). Inhibitory effects of lactoferrin on biofilm formation in clinical isolates of Pseudomonas aeruginosa. Journal of Infection and Chemotherapy, 18(1), 47-52.
    • Kengne AP, Dzudie AI, Fezeu LL, Mbanya JC. (2006). Impact of secondary foot complications on the inpatient department of the diabetes unit of Yaounde Central Hospital.
    • International Journal of Lower Extremity Wounds. 5(1):64-8.
    • Keren, I., Kaldalu, N., Spoering, A., Wang, Y., Lewis, K. (2004). Persister cells and tolerance to antimicrobials. FEMS Microbiology Letters, 230(1), 13-18.
    • Keren, I., Mulcahy, L. R., Lewis, K. (2012). Persister eradication: lessons from the world of natural products. Methods Enzymol, 517, 387-406.
    • Khaw, K. T., Wareham, N., Luben, R., Bingham, S., Oakes, S., Welch, A., Day, N. (2001).
    • Glycated haemoglobin, diabetes, and mortality in men in Norfolk cohort of European Prospective Investigation of Cancer and Nutrition (EPIC-Norfolk). BMJ, 322(7277), 15.
    • King, H., Aubert, R. E., Herman, W. H. (1998). Global burden of diabetes, 1995-2025; prevalence, numerical estimates, and projections. Diabetes Care. 21, 1414-31 Kint, C. I., Verstraeten, N., Fauvart, M., Michiels, J. (2012). New-found fundamentals of bacterial persistence. Trends in Microbiology, 20(12), 577-585.
    • Kobold, U., Jeppsson, J. O., Dülffer, T., Finke, A., Hoelzel, W., Miedema, K. (1997).
    • Candidate reference methods for hemoglobin A1c based on peptide mapping. Clinical Chemistry, 43 (10), 1944-1951.
    • Kolenbrander, P. E. (2000). Oral microbial communities: biofilms, interactions, and genetic systems 1. Annual Reviews in Microbiology, 54(1), 413-437.
    • Köser, C. U., Ellington, M. J., Peacock, S. J. (2014). Whole-genome sequencing to control antimicrobial resistance. Trends in Genetics, 30(9), 401-407.
    • Kyari, F., Tafida, A., Sivasubramaniam, S., Murthy, G. V., Peto, T., Gilbert, C. E. (2014).
    • Prevalence and risk factors for diabetes and diabetic retinopathy: results from the Nigeria national blindness and visual impairment survey. BMC public health, 14(1), 1299.
    • Lagacé-Wiens, P., Walkty, A., Karlowsky, A. J. (2014). Ceftazidime-avibactam: an evidence-based review of its pharmacology and potential use in the treatment of Gramnegative bacterial infections. Core Evidence. 9 13-25.
    • Landon, M. B. (2010). Is there a benefit to the treatment of mild gestational diabetes mellitus? Am J Obstet Gynecol. 202, 649-53 Lane, M. C., Li, X., Pearson, M. M., Simms, A. N., Mobley, H. L. (2009). Oxygen-limiting conditions enrich for fimbriate cells of uropathogenic Proteus mirabilis and Escherichia coli. Journal of Bacteriology, 191(5), 1382-1392.
    • Largay, J. (2012). Case Study: New-Onset Diabetes: How to Tell the Difference Between Type 1 and Type 2 Diabetes. Clinical Diabetes, 30(1), 25-26.
    • Latasa, C., Solano, C., Penades, J. R., Lasa, I. (2006). Biofilm-associated proteins. C R Biol 329: 849-857.
    • Lavery, L. A., Armstrong, D. G., Vela, S. A., Quebedeaux, T. L., Fleischli, J. G. (1998).
    • Practical criteria for screening patients at high risk for diabetic foot ulceration. Archives of Internal Medicine, 158(2), 157-162.
    • A. (2006). Risk factors for foot infections in individuals with diabetes. Diabetes Care, 29(6), 1288-1293.
    • Lavigne, J.-P., Sotto, A., Dunyach-Remy, C., Lipsky, B. A. (2015). New Molecular Techniques to Study the Skin Microbiota of Diabetic Foot Ulcers. Advances in Wound Care, 4(1), 38-49. (Available from: http://doi.org/10.1089/wound.2014.0532). Accessed 14/10/2015] Leadbetter, J. R., Greenberg, E. P. (2000). Metabolism of acylhomoserine lactone quorumsensing signals by Variovorax paradoxus. J. Bacteriol. 182, 6921-6926 Leibiger, I. B., Brismar, K. and Berggren, P. O. (2010). Novel aspects on pancreatic beta-cell signal-transduction. Biochem Biophys Res Commun. 396, 111-5.
    • Lembre, P., Lorentz, C., Di Martino, P. (2012). Exopolysaccharides of the biofilm matrix: A complex biophysical world. INTECH Open Access Publisher.
    • Lerat, E., Daubin, V., Moran, N. A. (2003). From Gene Trees to Organismal Phylogeny in Prokaryotes: The Case of the γ-Proteobacteria. PLoS Biol 1(1): e19 Lerat, E., Moran, N. A. (2004). The evolutionary history of quorum-sensing systems in bacteria. Molecular Biology and Evolution, 21(5), 903-913.
    • Levin, M. E. (1998). Classification of diabetic foot wounds. Diabetes Care. 21(5), 681-681.
    • Levitt, N. S. (2008). Diabetes in Africa: epidemiology, management and healthcare challenges. Heart. 94:1376-1382.
    • Lewandowski, Z. (2000). Structure and function of biofilms. In: Evans, L. V., (ed.) Biofilms: recent advances in their study and control. Amsterdam: Harwood Academic Publishers. P. 1- 17.
    • Lewis, K. (2001). Riddle of biofilm resistance. Antimicrob. Agens Chemother. 45 (4): 999- 1007.
    • Lewis, K. (2005). Persister cells and the riddle of biofilm survival. Biochemistry (Moscow), 70(2), 267-274.
    • Lewis, K. (2007). Persister cells, dormancy and infectious disease. Nature Reviews Microbiology, 5(1), 48-56.
    • Lewis, K. (2010). Persister cells. Annual Review of Microbiology, 64, 357-372.
    • Lewis, K. 2005. Persister cells and the riddle of biofilm survival. Biochemistry (Moscow) 70: 267-274.
    • Li, Y. H., Tian, X. (2012). Quorum sensing and bacterial social interactions in biofilms. Sensors, 12(3), 2519-2538.
    • Lilley, B. N., Bassler, B. L. (2000). Regulation of quorum sensing in Vibrio harveyi by LuxO and sigma‐54. Molecular Microbiology, 36(4), 940-954.
    • Ling, L. L., Schneider, T., Peoples, A. J., Spoering, A. L., Engels, I., Conlon, B. P., Mueller, A., Schäberle, T. F., Hughes, D. E., Epstein, S Jones, M. (2015). A new antibiotic kills pathogen without detectable resistance. Nature, 517(7535), 455-459.
    • (2012). Infectious Diseases Society of America clinical practice guideline for the diagnosis and treatment of diabetic foot infections. Clinical infectious diseases, 54(12), e132-e173.
    • Lipsky, B. A., Berendt, A. R., Deery, H. G., Embil, J. M., Joseph, W. S., Karchmer, A. W., LeFrock, J. L., Lew, D. P., Mader, J. T., Norden, C., Tan, J. S. (2004). Diagnosis and Treatment of Diabetic Foot Infections. Clinical Infectious Diseases, 39 (7), 885-910 Lipsky, B. A., Richard, J.-L., Lavigne, J.-P. (2013). Diabetic Foot Ulcer Microbiome: One Small Step for Molecular Microbiology . . . One Giant Leap for Understanding Diabetic Foot Ulcers? Diabetes, 62(3), Lis, H., Sharon, N. (1998). Lectins: carbohydrate-specific proteins that mediate cellular recognition. Chemical reviews, 98(2), 637-674.
    • Liu, Y., Tay, J. H. (2001). Metabolic response of biofilm to shear stress in fixed‐film culture. Journal of Applied Microbiology, 90 (3), 337-342.
    • Llamas, I., Keshavan, N., Gonzalez, J. E. (2004) Use of Sinorhizobium meliloti as an indicator for specific detection of long-chain N-acyl homoserine lactones. Appl Environ Microbiol 70:3715-3723.
    • Loeb, G. I., Neihof, R. A. (1975). Marine conditioning films. Advances in Chemistry 145:319-35 Loo, A. E. K., Wong, Y. T., Ho, R., Wasser, M., Du, T., Ng, W. T., Halliwell, B. (2012).
    • Effects of hydrogen peroxide on wound healing in mice in relation to oxidative damage. PloS One, 7 (11), e49215.
    • Lopez, D., Vlamakis, H., Kolter, R. (2010). Biofilms. Cold Spring Harb Perspect Biol 2: a000398.
    • Losos, J. B. (2008). Phylogenetic niche conservatism, phylogenetic signal and the relationship between phylogenetic relatedness and ecological similarity among species. Ecology Letters. 11(10), 995-1003.
    • Low, E. W., Chase, H. A., Milner, M. G., Curtis, T. P. (2000). Uncoupling of metabolism to reduce biomass production in the activated sludge process. Water Research, 34 (12), 3204- 3212.
    • F., Miller, S. I., Kaufmann, G. F., Janda, K. D. (2013). A chemical biology approach to interrogate quorum-sensing regulated behaviors at the molecular and cellular level.
    • Chemistry & Biology, 20(7), 903-911.
    • Lowery, C. A., Park, J., Kaufmann, G. F., Janda, K. D. (2008). An unexpected switch in the modulation of AI-2-based quorum sensing discovered through synthetic 4, 5-dihydroxy-2, 3-pentanedione analogues. Journal of the American Chemical Society, 130(29), 9200-9201.
    • 271 Luidalepp, H., Jõers, A., Kaldalu, N., Tenson, T. (2011). Age of inoculum strongly influences persister frequency and can mask effects of mutations implicated in altered persistence.
    • Journal of Bacteriology, 193(14), 3598-3605.
    • (2009). Cigarette smoking and pancreatic cancer: a pooled analysis from the pancreatic cancer cohort consortium. Am J Epidemiol. 170(4):403-13.
    • E. (2012). Explaining the Increase of Diabetes Prevalence and Plasma Glucose in Mauritius. Diabetes Care, 35(1), 87-91.
    • Magnusson, I., Rothman, D. L., Katz, L. D., Shulman, R. G., Shulman, G. I. (1992).
    • Increased rate of gluconeogenesis in type II diabetes mellitus. A 13C nuclear magnetic resonance study. Journal of Clinical Investigation, 90(4), 1323-1327.
    • Mah, T. F., O'Toole, G. A. (2001). Mechanisms of biofilm resistance to antimicrobial agents.
    • Trends Microbiol 9: 34-39.
    • Maisonneuve, E., Gerdes, K. (2014). Molecular mechanisms underlying bacterial persisters. Cell, 157(3), 539-548.
    • Malik, A., Mohammad, Z., Ahmad, J. (2013). The diabetic foot infections: biofilms and antimicrobial resistance. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 7(2), 101-107.
    • Marsh, P. D. (1995). Dental plaque. In: Lappin-Scott, H. M., Costerton J. W. (ed.) Microbial biofilms. Cambridge: Cambridge University Press, 282-300.
    • Marshall, K. C., Cruickshank, R. H. (1973). Cell surface hydrophobicity and the orientation of certain bacteria at interfaces. Archiv Für Mikrobiologie, 91 (1), 29-40.
    • Marshall, K. C., Stout, R., Mitchell, R. (1971). Mechanism of the initial events in the sorption of marine bacteria to surfaces. Microbiology, 68 (3), 337-348.
    • Martin, J. M., Zenilman, J. M., Lazarus, G. S. (2010). Molecular microbiology: new dimensions for cutaneous biology and wound healing. Journal of Investigative Dermatology, 130(1), 38-48.
    • (2002). Functional blocking of Staphylococcus aureus adhesins following growth in ex vivo media. Infection and Immunity, 70 (10), 5339-5345.
    • Matcher, G. F., Jiwaji, M., de la Mare, J. A., Dorrington, R. A. (2013). Complex pathways for regulation of pyrimidine metabolism by carbon catabolite repression and quorum sensing in Pseudomonas putida RU-KM3S. Applied Microbiology and Biotechnology, 97(13), 5993- 6007.
    • Matsukawa, M., Greenberg, E. P. (2004). Putative exopolysaccharide synthesis genes influence Pseudomonas aeruginosa biofilm development. Journal of Bacteriology, 186(14), 4449-4456.
    • Mayon-White, R. T., Ducel, G., Kereselidze, T., Tikomirov, E. (1988). An international survey of the prevalence of hospital-acquired infection. Journal of Hospital Infection. 11 (suppl A), 43-48.
    • Mayrand, D., McBride, B. C. (1980). Ecological relationships of bacteria involved in a simple, mixed anaerobic infection. Infection and Immunity, 27(1), 44-50.
    • McCane, D. R., Hanson, R. L., Charles, M. A., Jacobsson, L. T., Pettitt, D. D., Bennett, P. H., Knowler, W. C. (1994). Comparison of tests for glycated haemoglobin and fasting and twohour plasma glucose concentrations as diagnostic methods for diabetes. BMJ, 308(6940), 1323-1328.
    • McClean, K. H., Winson, M. K., Fish, L., Taylor, A., Chhabra, S. R., Camara, M., Daykin, M., Lamb, J. H., Swift, S., Bycroft, B. W., Stewart, G. S. A., Williams, P. (1997). Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology, 143 (12), 3703-3711.
    • McDonnell, G., Russell, A. D. (1999). Antiseptics and Disinfectants: Activity, Action, and Resistance. Clin Microbiol Rev. 12 (1): 147-179.
    • McIntire, F. C., Vatter, A. E., Baros, J., Arnold, J. (1978). Mechanism of coaggregation between Actinomyces viscosus T14V and Streptococcus sanguis 34. Infection and Immunity, 21 (3), 978-988.
    • McLean, R. J. C., Pierson, L. S., Fuqua, C. (2004). A simple screening protocol for the identification of quorum sensing antagonists. J Microbiol Methods. 58, 351-60 Meighen, E. A. (1991). Molecular biology of bacterial bioluminescence. Microbiological Reviews, 55(1), 123-142.
    • Meighen, E. A. (1993). Bacterial bioluminescence: organization, regulation, and application of the lux genes. The FASEB Journal, 7(11), 1016-1022.
    • Mellefont, L. A., McMeekin, T. A., Ross, T. (2008). Effect of relative inoculum concentration on Listeria monocytogenes growth in co-culture. International Journal of Food Microbiology, 121(2), 157-168.
    • Merritt, J. H., Kadouri, D. E., O'Toole, G. (2011). Growing and Analyzing Static Biofilms.
    • Current Protocols in Microbiology. Supplement 22, 1B.1.1-1B.1.18 Millar, B. C., Jiru, X., Moore, J. E., Earle, J. A. P. (2000). A simple and sensitive method to extract bacterial, yeast and fungal DNA from blood culture material. Journal of Microbiological Methods 42, 139-147.
    • L., Hughson, F. M. (2004). Salmonella typhimurium recognizes a chemically distinct form of the bacterial quorum-sensing signal AI-2. Molecular Cell, 15(5), 677-687.
    • Milne, S. D., Connolly, P. (2014). The influence of different dressings on the pH of the wound environment. Journal of Wound Care, 23 (2), 53-57.
    • Mingeot-Leclercq, M. P., Glupczynski, Y., Tulkens, P.M., (1999). Aminoglycosides: Activity and Resistance. Antimicrob Agents Chemother 43 (4):727-737.
    • Munoz, A., Alonso, B., Alvarez, O., Llovo, J. (2003). Lectin typing of five medically important Candida species. Mycoses, 46 (3‐4), 85-89.
    • Munoz, A., Alvarez, O., Alonso, B., Llovo, J. (1999). Lectin typing of methicillin-resistant Staphylococcus aureus. Journal of Medical Microbiology, 48(5), 495-499.
    • Murray, B. E. (1991). New aspects of antimicrobial resistance and the resulting therapeutic dilemmas. Journal of Infectious Diseases, 163(6), 1185-1194.
    • Nakagami, G., Sanada, H., Sugama, J., Morohoshi, T., Ikeda, T., Ohta, Y. (2008). Detection of Pseudomonas aeruginosa quorum sensing signals in an infected ischemic wound: an experimental study in rats. Wound Repair and Regeneration, 16 (1), 30-36.
    • Nather, A., Bee, C. S., Huak, C. Y., Chew, J. L., Lin, C. B., Neo, S., Sim, E. Y. (2008).
    • Epidemiology of diabetic foot problems and predictive factors for limb loss. Journal of Diabetes and its Complications, 22(2), 77-82.
    • (1999). Genetic approaches to study of biofilms. Methods Enzymol. 310:91-109.
    • Oates, A., Bowling, F. L., Boulton, A. J., McBain, A. J. (2012). Molecular and culture-based assessment of the microbial diversity of diabetic chronic foot wounds and contralateral skin sites. Journal of Clinical Microbiology, 50(7), 2263-2271.
    • Ochman, H., Lawrence, J. G., Groisman, E. A. (2000). Lateral gene transfer and the nature of bacterial innovation. Nature. 405(6784), 299-304.
    • Ofek, I., Doyle, R. J. (1994). Bacterial adhesion to cells and tissues. 735. New York: Chapman & Hall.
    • Ogbera, A. O., Fasanmade, O., Ohwovoriole, A. E., Adediran, O. (2006). An assessment of the disease burden of foot ulcers in patients with diabetes mellitus attending a teaching hospital in Lagos, Nigeria. The International Journal of Lower Extremity Wounds, 5(4), 244- 249.
    • Olson, M.E., Ceri, H., Morck, D.W., Buret, A.G. and Read, R.R. (2002) Biofilm bacteria: formation and comparative susceptibility to antibiotics. Canadian Journal of Veterinary Research 66, 86-92.
    • O'Meara, S., Cullum, N., Majid, M., Sheldon, T. (2000). Systematic reviews of wound care management: (3) antimicrobial agents for chronic wounds; (4) diabetic foot ulceration.
    • Health Technology Assessment (Winchester, England). 4(21):1-237.
    • Orrett, F. A., Brooks, P. J., Richardson, E. G. (1998). Nosocomial infections in a rural regional hospital in a developing country: infection rates by site, service, cost, and infection control practices. Infection Control. 19(02), 136-140.
    • O'Toole, G. A., Kolter, R. (1998). Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Molecular Microbiology, 30(2), 295-304.
    • O'Toole, G., Kaplan, H. B., Kolter, R. (2000). Biofilm formation as microbial development.
    • Annual Reviews in Microbiology, 54(1), 49-79.
    • Owiredu, W. K. B. A., Adamu, M. S., Amidu, N., Woode, E., Bam, V., Plange-Rhule, J., Opoku-Okrah, C. (2008). Obesity and cardiovascular risk factors in a pentecostal population in Kumasi-Ghana. J Med Sci, 8, 682-690.
    • (2014). A multistep procedure to prepare pre-vascularized cardiac tissue constructs using adult stem cells, dynamic cell cultures, and porous scaffolds. Frontiers in Physiology, 5: 210.
    • Papasian, C. J., Kragel, P. J. (1997). The microbiology laboratory's role in life-threatening infections. Critical Care Nursing Quarterly. 20(3), 44-59.
    • Schreml, S., Landthaler, M., Schäferling, M., Babilas, P. (2011). A new star on the H2O2rizon of wound healing? Experimental Dermatology, 20 (3), 229-231.
    • Schuster, M., Greenberg, E. P. (2006). A network of networks: quorum-sensing gene regulation in Pseudomonas aeruginosa. International Journal of Medical Microbiology, 296(2), 73-81.
    • Seckam, A., Cooper, R. (2013). Understanding how honey impacts on wounds: an update on recent research findings. Wounds International. 4(1): 20-24 Shirtliff, M. E., Mader, J. T., Camper, A. K. (2002). Molecular Interactions in Biofilms.
    • Chemistry and Biology. 9, 859-71 Singh, N., Armstrong, D. G., Lipsky, B. A. (2005). Preventing foot ulcers in patients with diabetes. JAMA. 293(2):217-28 Siripong, P., Chuleekorn, S., Duangporn, P. (2014). Enhanced cellulose production by ultraviolet (UV) irradiation and N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis of an Acetobacter species isolate. African Journal of Biotechnology, 11(6), 1433-1442.
    • Slifkin, M., Doyle, R. J. (1990). Lectins and their application to microbiology. Clinical Microbiology Reviews, 3(3), 197-218.
    • Van Loosdrecht, M. C., Lyklema, J., Norde, W., Schraa, G., Zehnder, A. J. (1987). The role of bacterial cell wall hydrophobicity in adhesion. Applied and Environmental Microbiology, 53 (8), 1893-1897.
    • Wolcott, R. D., Rhoads, D. D., Dowd, S. E. (2008). Biofilms and chronic wound inflammation. J Wound Care, 17 (8), 333-341.
    • Wolf, Y. I., Rogozin, I. B., Grishin, N. V., Tatusov, R. L., Koonin, E. V. (2001). Genome trees constructed using five different approaches suggest new major bacterial clades. BMC Evolutionary Biology, 1(1), 1.
    • Wood, K. T., Knabel, S. J., Kwan, B. W. (2013). Bacterial Persister Cell Formation and Dormancy. Applied and Environmental Microbiology. 79, 7116-7121 Zhu, J., Beaber, J. W., Moré, M. I., Fuqua, C., Eberhard, A., Winans, S. C. (1998). Analogs of the autoinducer 3-oxooctanoyl-homoserine lactone strongly inhibit activity of the TraR protein of Agrobacterium tumefaciens. Journal of Bacteriology, 180(20), 5398-5405.
    • Zhu, J., Dizin, E., Hu, X., Wavreille, A. S., Park, J., Pei, D. (2003). S-Ribosylhomocysteinase (LuxS) is a mononuclear iron protein. Biochemistry, 42(16), 4717-4726.
    • Zhu, J., Mekalanos, J. J. (2003). Quorum sensing-dependent biofilms enhance colonization in Vibrio cholerae. Developmental Cell, 5(4), 647-656.
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