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Dzidzienyo, Daniel K.; Bryan, Glenn J.; Wilde, Gail; Robbins, Timothy P. (2016)
Publisher: Springer Nature
Journal: Theoretical and Applied Genetics
Languages: English
Types: Article
Subjects: Agronomy and Crop Science, Original Article, Biotechnology, Genetics

Classified by OpenAIRE into

mesheuropmc: food and beverages
Key message The S-ribonuclease sequences of 16 S-alleles derived from diploid types of Solanum are presented. A phylogenetic analysis and partial phenotypic analysis support the conclusion that these are functional S-alleles. Abstract S-Ribonucleases (S-RNases) control the pistil specificity of the self-incompatibility (SI) response in the genus Solanum and several other members of the Solanaceae. The nucleotide sequences of S-RNases corresponding to a large number of S-alleles or S-haplotypes have been characterised. However, surprisingly, few S-RNase sequences are available for potato species. The identification of new S-alleles in diploid potato species is desirable as these stocks are important sources of traits such as biotic and abiotic resistance. S-RNase sequences are reported here from three distinct diploid types of potato: cultivated Solanum tuberosum Group Phureja, S. tuberosum Group Stenotomum, and the wild species Solanum okadae. Partial S-RNase sequences were obtained from pistil RNA by RT-PCR or 3′RACE (Rapid Amplification of cDNA Ends) using a degenerate primer. Full-length sequences were obtained for two alleles by 5′RACE. Database searches with these sequences identified 16 S-RNases in total, all of which are novel. The sequence analysis revealed all the expected features of functional S-RNases. Phylogenetic analysis with selected published S-RNase and S-like-RNase sequences from the Solanaceae revealed extensive trans-generic evolution of the S-RNases and a clear distinction from S-like-RNases. Pollination tests were used to confirm the self-incompatibility status and cross-compatibility relationships of the S. okadae accessions. All the S. okadae accessions were found to be self-incompatible as expected with crosses amongst them exhibiting both cross-compatibility and semi-compatibility consistent with the S-genotypes determined from the S-RNase sequence data. The progeny analysis of four semi-compatible crosses examined by allele-specific PCR provided further confirmation that these are functional S-RNases. Electronic supplementary material The online version of this article (doi:10.1007/s00122-016-2754-7) contains supplementary material, which is available to authorized users.
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    • Allen AM, Hiscock SJ (2008) Evolution and phylogeny of selfincompatibility systems in angiosperms. In: Franklin-Tong VE (ed) Self-incompatibility in flowering plants: evolution, diversity and mechanisms. Springer, Berlin, pp 73-101
    • Anderson MA, Cornish EC, Mau SL, Williams EG, Hoggart R, Atkinson A, Bonig I, Grego B, Simpson R, Roche PJ, Haley JD, Penschow JD, Niall HD, Tregear GW, Coughlan JP, Crawford RJ, Clarke AE (1986) Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata. Nature 321:38-44
    • Asquini E, Gerdol M, Gasperini D, Igic B, Graziosi G, Pallavicini A (2011) S-RNase-like sequences in styles of Coffea (Rubiaceae). Evidence for S-RNase based gametophytic self-incompatibility? Trop Plant Biol 4(3):237-249
    • Bredemeijer GM, Blass J (1981) S-specific proteins in styles of selfincompatible Nicotiana alata. Theor Appl Genet 59:185-190
    • Broothaerts W, Vanvinckenroye P, Decock B, Van Damme J, Vendrig J (1991) Petunia hybrida S-proteins: ribonuclease activity and the role of their glycan side chains in self-incompatibility. Sex Plant Reprod 4:258-266
    • Cipar MS, Peloquin SJ, Hougas RW (1964) Inheritance of incompatibility in hybrids between Solanum tuberosum haploids and diploid species. Euphytica 13:163-172
    • de Nettancourt D (1977) Incompatibility in angiosperms. Springer, Berlin
    • de Nettancourt D (1997) Incompatibility in angiosperms. Sex Plant Reprod 10:185-199
    • de Nettancourt D (2001) Incompatibility and incongruity in wild and cultivated plants. Springer, Berlin
    • Despres C, Saba-El-Leil MK, Rivard SR, Morse D, Cappadocia M (1994) Molecular cloning of two Solanum chacoense S-alleles and a hypothesis concerning their evolution. Sex Plant Reprod 7:169-176
    • Dodds KS (1962) Classification of cultivated potatoes. In: Correll DS (ed) The potato and its wild relatives. Texas Research Foundation, Renner, pp 517-539
    • Dodds PN, Clarke AE, Newbigin E (1996) A molecular perspective on pollination in flowering plants. Cell 85:141-144
    • Emerson S (1939) A preliminary survey of the Oenothera organensis population. Genetics 24:524-537
    • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791
    • Franklin-Tong VE, Franklin FCH (2003) Gametophytic self-incompatibility inhibits pollen tube growth using different mechanisms. Trends Plant Sci 12:598-605
    • Hiscock SJ, McInnis SM (2003) The diversity of self-incompatibility systems in flowering plants. Plant Biol 5:23-32
    • Hugot K, Ponchet M, Marais A, Ricci P, Galiana E (2002) A tobacco S-like RNase inhibits hyphal elongation of plant pathogens. Mol Plant Microbe Interact 15:243-250
    • Ida K, Norioka S, Yamamoto M, Kumasaka T, Yamashita E, Newbigin E, Clarke AE, Sakiyama F, Sato M (2001) The 1.55 A resolution structure of Nicotiana alata SF11-RNase associated with gametophytic self-incompatibility. J Mol Biol 314:103-112
    • Igic B, Kohn JR (2001) Evolutionary relationships among self-incompatibility RNases. Proc Natl Acad Sci USA 98:13167-13171
    • Igic B, Bohs L, Kohn JR (2003) Historical inferences from the selfincompatibility locus. New Phytol 161:97-105
    • Ioerger TR, Clark AG, Kao TH (1990) Polymorphism at the selfincompatibility locus in Solanaceae predates speciation. Proc Natl Acad Sci USA 87:9732-9735
    • Ioerger TR, Gohlke JR, Xu B, Kao TH (1991) Primary structural features of the self-incompatibility protein in Solanaceae. Sex Plant Reprod 4:81-87
    • Ishimizu T, Norioka S, Kanai M, Clarke AE, Sakiyama F (1996) Location of cysteine and cystine residues in S-ribonucleases associated with gametophytic self-incompatibility. Eur J Biochem 242:627-635
    • Kao TH, McCubbin AG (1996) How flowering plants discriminate between self and non-self pollen to prevent inbreeding. Proc Natl Acad Sci USA 93:12059-12065
    • Karunanandaa B, Huang S, Kao T (1994) Carbohydrate moiety of the Petunia inflata S3 protein is not required for self-incompatibility interactions between pollen and pistil. Plant Cell 6:1933-1940
    • Kaufmann H, Salamini F, Thompson RD (1991) Sequence variability and gene structure at the self-incompatibility locus of Solanum tuberosum. Mol Gen Genet 226:457-466
    • Kirch HH, Uhrig H, Lottspeich F, Salamini F, Thompson RD (1989) Characterization of proteins with self-incompatibility in Solanum tuberosum. Theor Appl Genet 78:581-588
    • Lee HS, Singh A, Kao TH (1992) RNase X2, a pistil specific ribonuclease from Petunia inflata, shares sequence similarity with solanaceous S proteins. Plant Mol Biol 20:1131-1141
    • Lee HS, Huang S, Kao TH (1994) S proteins control rejection of incompatible pollen in Petunia inflata. Nature 367:560-563
    • Marcellan ON, Acevedo A, Camadro EL (2006) S16, a novel S-RNase allele in the diploid species Solanum chacoense. Genome 49(8):1052-1054
    • McClure BA, Haring V, Ebert PR, Anderson MA, Simpson RJ, Sakiyama F, Clarke AE (1989) Style self-incompatibility gene products of Nicotiana alata are ribonucleases. Nature 342:955-957
    • McCubbin AG, Kao TH (2000) Molecular recognition and response in pollen and pistil interactions. Annu Rev Cell Dev Biol 16:333-364
    • Murfett J, Atherton T, Mou B, Gasser C, McClure B (1994) S-RNase expressed in transgenic Nicotiana causes S-allele-specific pollen rejection. Nature 367:563-566
    • Nowak MD, Davis AP, Anthony F, Yoder AD (2011) Expression and trans-specific polymorphism of self-incompatibility RNases in Coffea (Rubiaceae). PLoS ONE 6:e21019. doi:10.1371/journal. pone.0021019
    • O'Brien M, Kapfer C, Major G, Laurin M, Bertrand C, Kondo K, Kowyama Y, Matton DP (2002) Molecular analysis of the stylar-expressed Solanum chacoense small asparagine-rich protein family related to the HT modifier of gametophytic self-incompatibility in Nicotiana. Plant J 32(6):985-996
    • Oxley D, Munro SL, Craik DJ, Bacic A (1998) Structure and distribution of N-glycans on the S7-allele stylar self-incompatibility ribonuclease of Nicotiana alata. J Biochem 123:978-983
    • Pandey KK (1962) Interspecific incompatibility in Solanum species. Am J Bot 49:874-882
    • Pushkarnath (1942) Studies on sterility in potatoes. 1. The genetics of self- and cross-incompatibilities. Indian J Genet Plant Breed 2:11-36
    • Qi X, Luu D, Yang Q, Maes O, Matton D, Morse D, Cappadocia M (2001) Genotype-dependent differences in S12-RNase expression lead to sporadic self-compatibility in Solanum chacoense. Plant Mol Biol 45:295-305
    • Richman AD, Kohn JR (1999) Self-incompatibility alleles from Physalis: implications for historical inference from balanced genetic polymorphisms. Proc Natl Acad Sci USA 96:168-172
    • Richman AD, Kohn JR (2000) Evolutionary genetics of self-incompatibility in the Solanaceae. Plant Mol Biol 42:169-179
    • Richman AD, Uyenoyama MK, Kohn JR (1996) Allelic diversity and gene genealogy at the self-incompatibility locus in the Solanaceae. Science 273:1212-1216
    • Roalson EH, McCubbin AG (2003) S-RNases and sexual incompatibility: structure, functions, and evolutionary perspectives. Mol Phylogenet Evol 29:490-506
    • Roldan JA, Quiroga R, Goldraij A (2010) Molecular and genetic characterization of novel S-RNases from a natural population of Nicotiana alata. Plant Cell Rep 29:735-746
    • Saba-el-Leil MK, Rivard S, Morse D, Cappadocia M (1994) The S11 and S13 self incompatibility alleles in Solanum chacoense Bitt. are remarkably similar. Plant Mol Biol 24(4):571-583
    • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406-425
    • Silva NF, Goring DR (2001) Mechanisms of self-incompatibility in lfowering plants. Cell Mol Life Sci 58:1988-2007
    • Spooner DM, Ghislain M, Simon R, Jansky SH, Gavrilenko T (2014) Systematics, diversity, genetics, and evolution of wild and cultivated potatoes. Bot Rev 80:283-383
    • Stone JL, Pierce SE (2005) Rapid recent radiation of S-RNase lineages in Witheringia solanacea (Solanaceae). Heredity 94:547-555
    • Sutherland BG, Tobutt K, Robbins TP (2008) Trans-specific S-RNase and SFB alleles in Prunus self-incompatibility haplotypes. Mol Genet Genom 279:95-106
    • Takayama S, Isogai A (2005) Self-incompatibility in plants. Ann Rev Plant Biol 56:467-489
    • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731-2739
    • Thompson J, Higgins D, Gibson T, Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680
    • Wheeler MJ, de Graaf BHJ, Hadjiosif NE, Perry RM, Poulter NS, Osman K, Vatovec S, Harper A, Franklin FCH, Franklin-Tong VE (2009) Identification of the pollen self-incompatibility deter - minant in Papaver rhoeas. Nature 459:992-995
    • Xu BB, Mu JH, Nevins DL, Grun P, T-h Kao (1990) Cloning and sequencing of cDNAs encoding two self-incompatibility associated proteins in Solanum chacoense. Mol Gen Genet 224(3):341-346
    • Xue Y, Carpenter R, Dickinson HG, Coen ES (1996) Origin of allelic diversity in Antirrhinum S Locus RNases. Plant Cell 8(5):805-814
    • Zuckerkandl E, Pauling L (1965) Evolutionary divergence and convergence in proteins. In: Bryson V, Vogel HJ (eds) Evolving genes and proteins. Academic Press, New York, pp 97-166
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