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Publisher: PLOS
Languages: English
Types: Article
Subjects: QR

Classified by OpenAIRE into

mesheuropmc: complex mixtures, fungi, bacteria
An attempt was made to verify the observation that Streptomyces griseus was prevalent in soil based on isolation work. A genus-specific PCR was developed for Streptomyces based on the housekeeping gene atpD and used to investigate species diversity within selected soils. The presence of S. griseus was investigated to determine coexistence of resistance-only streptomycin phosphotransferase (strA) in the same soil as streptomycin producers. Two additional PCR-based assays were developed; one specific for strA in association with production, the other for more diverse strA and other related phosphotranferases. Both the S. griseus atpD and strA genes were below the PCR detection limit in all soils examined. A number of more diverse phosphotransferase genes were amplified, a minority of which may be associated with streptomycin production. We conclude that neither streptomycin producers nor S. griseus are prevalent in the fresh or chitin and starch-amended soils examined (less than 0.1% of soil actinobacteria). One of the soil sites had received plantomycin (active ingredient: streptomycin) and diversity studies suggested that this altered the streptomycete populations present in the soil.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1. Euzeby JP (2012) List of Prokaryotic names with Standing in Nomenclature - Genus Streptomyces. Retrieved 20th of February, 2012, from http://www. bacterio.cict.fr/s/streptomycesb.html.
    • 2. Williams ST, Goodfellow M, Alderson G, Wellington EM, Sneath PH, et al. (1983) Numerical classification of Streptomyces and related genera. J Gen Microbiol 129: 1743-1813.
    • 3. Anderson AS, Wellington EM (2001) The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol 51: 797-814.
    • 4. Staley JT (2006) The bacterial species dilemma and the genomic-phylogenetic species concept. Philos Trans R Soc Lond B Biol Sci 361: 1899-1909.
    • 5. Goodfellow M, Kumar Y, Labeda DP, Sembiring L (2007) The Streptomyces violaceusniger clade: a home for Streptomycetes with rugose ornamented spores. Antonie Van Leeuwenhoek 92: 173-199.
    • 6. Kataoka M, Ueda K, Kudo T, Seki T, Yoshida T (1997) Application of the variable region in 16 S rDNA to create an index for rapid species identification in the genus Streptomyces. FEMS Microbiol Lett 151: 249-255.
    • 7. Kumar Y, Goodfellow M (2008) Five new members of the Streptomyces violaceusniger 16 S rRNA gene clade: Streptomyces castelarensis sp. nov., comb. nov., Streptomyces himastatinicus sp. nov., Streptomyces mordarskii sp. nov., Streptomyces rapamycinicus sp. nov. and Streptomyces ruanii sp. nov. Int J Syst Evol Microbiol 58: 1369-1378.
    • 8. Liu Z, Shi Y, Zhang Y, Zhou Z, Lu Z, et al. (2005) Classification of Streptomyces griseus (Krainsky 1914) Waksman and Henrici 1948 and related species and the transfer of 'Microstreptospora cinerea' to the genus Streptomyces as Streptomyces yanii sp. nov. Int J Syst Evol Microbiol 55: 1605-1610.
    • 9. Hain T, Ward-Rainey N, Kroppenstedt RM, Stackebrandt E, Rainey FA (1997) Discrimination of Streptomyces albidoflavus strains based on the size and number of 16 S-23 S ribosomal DNA intergenic spacers. Int J Syst Bacteriol 47: 202-206.
    • 10. Lanoot B, Vancanneyt M, Hoste B, Vandemeulebroecke K, Cnockaert MC, et al. (2005) Grouping of streptomycetes using 16 S-ITS RFLP fingerprinting. Research in Microbiology 156: 755-762.
    • 11. Tamegai H, Kuki T, Udagawa Y, Aoki R, Nagaya A, et al. (2006) Exploration of genes that encode a carbocycle-forming enzyme involved in biosynthesis of aminoglycoside antibiotics from the environmental. Bioscience Biotechnology and Biochemistry 70: 1711-1716.
    • 12. Gharaibeh R, Saadoun I, Mahasneh A (2003) Evaluation of combined 16 S rDNA and strb1 gene targeted PCR to identify and detect streptomycinproducing Streptomyces. J Basic Microbiol 43: 301-311.
    • 13. Kim BJ, Kim CJ, Chun J, Koh YH, Lee SH, et al. (2004) Phylogenetic analysis of the genera Streptomyces and Kitasatospora based on partial RNA polymerase beta-subunit gene (rpoB) sequences. Int J Syst Evol Microbiol 54: 593-598.
    • 14. Mun HS, Oh EJ, Kim HJ, Lee KH, Koh YH, et al. (2007) Differentiation of Streptomyces spp. which cause potato scab disease on the basis of partial rpoB gene sequences. Systematic and Applied Microbiology 30: 401-407.
    • 15. Egan S, Wiener P, Kallifidas D, Wellington EM (2001) Phylogeny of Streptomyces species and evidence for horizontal transfer of entire and partial antibiotic gene clusters. Antonie Van Leeuwenhoek 79: 127-133.
    • 16. Guo Y, Zheng W, Rong X, Huang Y (2008) A multilocus phylogeny of the Streptomyces griseus 16 S rRNA gene clade: use of multilocus sequence analysis for streptomycete systematics. Int J Syst Evol Microbiol 58: 149-159.
    • 17. Laskaris P, Tolba S, Calvo-Bado L, Wellington EM (2010) Coevolution of antibiotic production and counter-resistance in soil bacteria. Environ Microbiol 12: 783-796.
    • 18. Rong X, Huang Y (2009) Taxonomic evaluation of the Streptomyces griseus clade using multilocus sequence analysis and DNA-DNA hybridization, with proposal to combine 29 species and three subspecies as 11 genomic species. Int J Syst Evol Microbiol 60: 696-703.
    • 19. Hanage WP, Fraser C, Spratt BG (2005) Fuzzy species among recombinogenic bacteria. Bmc Biology 3: 6.
    • 20. Babalola OO, Kirby BM, Le Roes-Hill M, Cook AE, Cary SC, et al. (2009) Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils. Environ Microbiol 11: 566-576.
    • 21. Chanal A, Chapon V, Benzerara K, Barakat M, Christen R, et al. (2006) The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria. Environ Microbiol 8: 514-525.
    • 22. Holmes AJ, Bowyer J, Holley MP, O'Donoghue M, Montgomery M, et al. (2000) Diverse, yet-to-be-cultured members of the Rubrobacter subdivision of the Actinobacteria are widespread in Australian arid soils. FEMS Microbiol Ecol 33: 111-120.
    • 23. Smith JJ, Tow LA, Stafford W, Cary C, Cowan DA (2006) Bacterial diversity in three different Antarctic Cold Desert mineral soils. Microb Ecol 51: 413-421.
    • 24. Stach JE, Maldonado LA, Ward AC, Goodfellow M, Bull AT (2003) New primers for the class Actinobacteria: application to marine and terrestrial environments. Environ Microbiol 5: 828-841.
    • 25. Valenzuela-Encinas C, Neria-Gonzalez I, Alcantara-Hernandez RJ, EstradaAlvarado I, Zavala-Diaz de la Serna FJ, et al. (2009) Changes in the bacterial populations of the highly alkaline saline soil of the former lake Texcoco (Mexico) following flooding. Extremophiles 13: 609-621.
    • 26. D'Costa VM, McGrann KM, Hughes DW, Wright GD (2006) Sampling the antibiotic resistome. Science 311: 374-377.
    • 27. Berdy J (2005) Bioactive microbial metabolites. J Antibiot (Tokyo) 58: 1-26.
    • 28. Jones D, Metzger HJ, Schatz A, Waksman SA (1944) Control of Gram-Negative Bacteria in Experimental Animals by Streptomycin. Science 100: 103-105.
    • 29. McManus PS, Stockwell VO, Sundin GW, Jones AL (2002) Antibiotic use in plant agriculture. Annual Review of Phytopathology 40: 443-+.
    • 30. Sundin GW, Monks DE, Bender CL (1995) Distribution of the StreptomycinResistance Transposon Tn5393 among Phylloplane and Soil Bacteria from Managed Agricultural Habitats. Canadian Journal of Microbiology 41: 792-799.
    • 31. Tolba S, Egan S, Kallifidas D, Wellington EM (2002) Distribution of streptomycin resistance and biosynthesis genes in streptomycetes recovered from different soil sites. FEMS Microbiol Ecol 42: 269-276.
    • 32. Garcia C, Hernandez T (2004) Site Description. Retrieved 20th of February 2012, from http://www.soil-index.com/english/documents/dl/index_site_ description.pdf.
    • 33. Baltz RH (2006) Marcel Faber Roundtable: is our antibiotic pipeline unproductive because of starvation, constipation or lack of inspiration? J Ind Microbiol Biotechnol 33: 507-513.
    • 34. Tolba S (2004) Distribution of streptomycin resistance and biosynthesis genes in streptomycetes recovered from different soil sites and the role of horizontal gene transfer in their dissemination. Coventry: University of Warwick.
    • 35. Metcalfe AC, Krsek M, Gooday GW, Prosser JI, Wellington EM (2002) Molecular analysis of a bacterial chitinolytic community in an upland pasture. Appl Environ Microbiol 68: 5042-5050.
    • 36. Pontiroli A, Travis ER, Sweeney FP, Porter D, Gaze WH, et al. (2011) Pathogen quantitation in complex matrices: a multi-operator comparison of DNA extraction methods with a novel assessment of PCR inhibition. PLoS One 6: e17916.
    • 37. Anderson AS, Clark DJ, Gibbons PH, Sigmund JM (2002) The detection of diverse aminoglycoside phosphotransferases within natural populations of actinomycetes. J Ind Microbiol Biotechnol 29: 60-69.
    • 38. Qu X, Wanner LA, Christ BJ (2008) Using the TxtAB operon to quantify pathogenic Streptomyces in potato tubers and soil. Phytopathology 98: 405-412.
    • 39. Manome A, Kageyama A, Kurata S, Yokomaku T, Koyama O, et al. (2008) Quantification of potato common scab pathogens in soil by quantitative competitive PCR with fluorescent quenching-based probes. Plant Pathology 57: 887-896.
    • 40. Schlatter DC, Samac DA, Tesfaye M, Kinkel LL (2010) Rapid and specific method for evaluating Streptomyces competitive dynamics in complex soil communities. Appl Environ Microbiol 76: 2009-2012.
    • 41. Gundlapally SR, Garcia-Pichel F (2006) The community and phylogenetic diversity of biological soil crusts in the Colorado Plateau studied by molecular fingerprinting and intensive cultivation. Microb Ecol 52: 345-357.
    • 42. Davelos AL, Xiao K, Samac DA, Martin AP, Kinkel LL (2004) Spatial variation in Streptomyces genetic composition and diversity in a prairie soil. Microb Ecol 48: 601-612.
    • 43. Wang Y, Zhang ZS, Ruan JS, Wang YM, Ali SM (1999) Investigation of actinomycete diversity in the tropical rainforests of Singapore. Journal of Industrial Microbiology & Biotechnology 23: 178-187.
    • 44. Monciardini P, Sosio M, Cavaletti L, Chiocchini C, Donadio S (2002) New PCR primers for the selective amplification of 16 S rDNA from different groups of actinomycetes. FEMS Microbiol Ecol 42: 419-429.
    • 45. Kormanec J, Farkasovsky M, Potuckova L, Godar S (1992) A gene (hur) from Streptomyces aureofaciens, conferring resistance to hydroxyurea, is related to genes encoding streptomycin phosphotransferase. Gene 114: 133-137.
    • 46. Aoki R, Nagaya A, Arakawa S, Kato C, Tamegai H (2008) Identification and diversity of putative aminoglycoside-biosynthetic aminotransferase genes from deep-sea environmental DNA. Biosci Biotechnol Biochem 72: 1388-1393.
    • 47. Nagaya A, Takeyama S, Tamegai H (2005) Identification of aminotransferase genes for biosynthesis of aminoglycoside antibiotics from soil DNA. Biosci Biotechnol Biochem 69: 1389-1393.
    • 48. Borjesson S, Dienues O, Jarnheimer PA, Olsen B, Matussek A, et al. (2009) Quantification of genes encoding resistance to aminoglycosides, beta-lactams and tetracyclines in wastewater environments by real-time PCR. Int J Environ Health Res 19: 219-230.
    • 49. Riesenfeld CS, Goodman RM, Handelsman J (2004) Uncultured soil bacteria are a reservoir of new antibiotic resistance genes. Environ Microbiol 6: 981-989.
    • 50. Casjens S (1998) The diverse and dynamic structure of bacterial genomes. Annu Rev Genet 32: 339-377.
    • 51. DSMZ (2012) Catalogue of microorganisms. Braunschweig. Available: http:// www.dsmz.de/catalogues/catalogue-microorganisms.html. Retrieved 20th of February 2012, 2012.
    • 52. Wink J (2012) Compendium of Actinobacteria. Braunschweig. Retrieved 20th of February 2012, from http://www.gbif-prokarya.de/microorganisms/wink.html.
    • 53. Calvo-Bado L Personal Communication.
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