LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Publisher: Wiley
Journal: LARYNGOSCOPE
Languages: English
Types: Article
Subjects: Article

Classified by OpenAIRE into

mesheuropmc: technology, industry, and agriculture, macromolecular substances
Objectives/Hypothesis: To develop a porous, biodegradable scaffold for mastoid air-cell regeneration.\ud Study Design: In vitro development of a temperature-sensitive poly(DL-lactic acid-co-glycolic acid)/poly(ethylene glycol) (PLGA/PEG) scaffold tailored for this application.\ud Methods: Human mastoid bone microstructure and porosity were investigated using micro-computed tomography.\ud PLGA/PEG-alginate scaffolds were developed, and scaffold porosity was assessed. Human bone marrow mesenchymal stem\ud cells (hBM-MSCs) were cultured on the scaffolds in vitro. Scaffolds were loaded with ciprofloxacin, and release of ciprofloxacin over time in vitro was assessed.\ud Results: Porosity of human mastoid bone was measured at 83% with an average pore size of 1.3 mm. PLGA/PEG-alginate\ud scaffold porosity ranged from 43% to 78% depending on the alginate bead content. The hBM-MSCs proliferate on the\ud scaffolds in vitro, and release of ciprofloxacin from the scaffolds was demonstrated over 7 to 10 weeks.\ud Conclusions: The PLGA/PEG-alginate scaffolds developed in this study demonstrate similar structural features to human mastoid bone, support cell growth, and display sustained antibiotic release. These scaffolds may be of potential clinical use in mastoid air-cell regeneration. Further in vivo studies to assess the suitability of PLGA/PEG-alginate scaffolds for this application are required.\ud Key Words: Scaffold, poly(DL-lactic acid-co-glycolic acid), alginate, mastoid, ciprofloxacin.\ud Level of Evidence: N/A.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1. Cinamon U, Sade J. Mastoid and tympanic membrane as pressure buffers: a quantitative study in a middle ear cleft model. Otol Neurotol 2003;24:839-842.
    • 2. Doyle WJ, Alper CM, Banks JM, Swarts JD. Rate of nitrous oxide exchange across the middle ear mucosa in monkeys before and after blockage of the mastoid antrum. Otolaryngol Head Neck Surg 2003;128:732-741.
    • 3. Kanemaru S, Nakamura T, Omori K, Magrufov A, Yamashita M, Ito J. Regeneration of mastoid air cells in clinical applications by in situ tissue engineering. Laryngoscope 2005;115:253-258.
    • 4. Howard D, Buttery LD, Shakesheff KM, Roberts SJ. Tissue engineering: strategies, stem cells and scaffolds. J Anat 2008;213:66-72.
    • 5. Dhillon A, Schneider P, Kuhn G, et al. Analysis of sintered polymer scaffolds using concomitant synchrotron computed tomography and in situ mechanical testing. J Mater Sci Mater Med 2011;22:2599-2605.
    • 6. Rahman CV, Ben-David D, Dhillon A, et al. Controlled release of BMP-2 from a sintered polymer scaffold enhances bone repair in a mouse calvarial defect model. J Tissue Eng Regen Med. In press.
    • 7. Bagot d'Arc M, Daculsi G, Emam N. Biphasic ceramics and fibrin sealant for bone reconstruction in ear surgery. Ann Otol Rhinol Laryngol 2004;113:711-720.
    • 8. Jang CH, Cho YB, Kim JS, Lee HJ, Kim GH. Regeneration of mastoid air cells using polycaprolactone/b-tricalcium phosphate biocomposites: an experimental study. Laryngoscope 2012;122:660-664.
    • 9. Silvola JT. Mastoidectomy cavity obliteration with bioactive glass. Otolaryngol Head Neck Surg 2012;147:119-126.
    • 10. Jang CH, Park H, Cho YB, Song CH. Mastoid obliteration using a hyaluronic acid gel to deliver a mesenchymal stem cells-loaded demineralized bone matrix: an experimental study. Int J Pediatr Otorhinolaryngol 2008;72:1627-1632.
    • 11. Nishizaki K, Tsujigiwa H, Takeda Y, et al. Mastoid obliteration by BMP-2/ collagen composites: an experimental study using tissue engineering. Am J Otolaryngol 2003;24:14-18.
    • 12. Puga AM, Rey-Rico A, Magarinos B, Alvarez-Lorenzo C, Concheiro A. Hot melt poly-epsilon-caprolactone/poloxamine implantable matrices for sustained delivery of ciprofloxacin. Acta Biomater 2012;8:1507-1518.
  • No related research data.
  • No similar publications.

Share - Bookmark

Published in

Funded by projects

  • EC | MASC
  • EC | BIODESIGN

Cite this article