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Publisher: American Society of Plant Biologists
Languages: English
Types: Article
Photomorphogenic responses triggered by low fluence rates of ultraviolet B radiation (UV-B; 280–315 nm) are mediated by the UV-B photoreceptor UV RESISTANCE LOCUS8 (UVR8). Beyond our understanding of the molecular mechanisms of UV-B perception by UVR8, there is still limited information on how the UVR8 pathway functions under natural sunlight. Here, wild-type Arabidopsis (Arabidopsis thaliana) and the uvr8-2 mutant were used in an experiment outdoors where UV-A (315–400 nm) and UV-B irradiances were attenuated using plastic films. Gene expression, PYRIDOXINE BIOSYNTHESIS1 (PDX1) accumulation, and leaf metabolite signatures were analyzed. The results show that UVR8 is required for transcript accumulation of genes involved in UV protection, oxidative stress, hormone signal transduction, and defense against herbivores under solar UV. Under natural UV-A irradiance, UVR8 is likely to interact with UV-A/blue light signaling pathways to moderate UV-B-driven transcript and PDX1 accumulation. UVR8 both positively and negatively affects UV-A-regulated gene expression and metabolite accumulation but is required for the UV-B induction of phenolics. Moreover, UVR8-dependent UV-B acclimation during the early stages of plant development may enhance normal growth under long-term exposure to solar UV.
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    • Plant Physiology , February 2013, Vol. 161, pp. 744-759, www.plantphysiol.org
    • 2012 American Society of Plant Biologists. All Rights Reserved. Two-color microarrays were used to identify genes regulated by solar UV
    • radiation in the wild type and uvr8-2 after 12 h of exposure outdoors. Two
    • Boccalandro HE, Rossi MC, Saijo Y, Deng XW, Casal JJ (2004) Promotion of photomorphogenesis by COP1. Plant Mol Biol 56: 905-915
    • Brosché M, Schuler MA, Kalbina I, Connor L, Strid Å (2002) Gene regulation by low level UV-B radiation: identification by DNA array analysis. Photochem Photobiol Sci 1: 656-664
    • Brown BA, Cloix C, Jiang GH, Kaiserli E, Herzyk P, Kliebenstein DJ, Jenkins GI (2005) A UV-B-specific signaling component orchestrates plant UV protection. Proc Natl Acad Sci USA 102: 18225-18230
    • Brown BA, Headland LR, Jenkins GI (2009) UV-B action spectrum for UVR8-mediated HY5 transcript accumulation in Arabidopsis. Photochem Photobiol 85: 1147-1155
    • Brown BA, Jenkins GI (2008) UV-B signaling pathways with different fluence-rate response profiles are distinguished in mature Arabidopsis leaf tissue by requirement for UVR8, HY5, and HYH. Plant Physiol 146: 576-588
    • Caldwell MM (1971) Solar UV irradiation and the growth and development of higher plants. In AC Giese, ed, Photophysiology. Vol VI. Current Topics in Photobiology and Photochemistry. Academic Press, New York, pp 131-177
    • Casati P, Campi M, Morrow DJ, Fernandes J, Walbot V (2011) Transcriptomic, proteomic and metabolomic analysis of maize responses to UV-B: comparison of greenhouse and field growth conditions. Plant Signal Behav 6: 1146-1153
    • Casati P, Walbot V (2003) Gene expression profiling in response to ultraviolet radiation in maize genotypes with varying flavonoid content. Plant Physiol 132: 1739-1754
    • Chen H, Xiong L (2005) Pyridoxine is required for post-embryonic root development and tolerance to osmotic and oxidative stresses. Plant J 44: 396-408
    • Christie JM, Arvai AS, Baxter KJ, Heilmann M, Pratt AJ, O'Hara A, Kelly SM, Hothorn M, Smith BO, Hitomi K, et al (2012) Plant UVR8 photoreceptor senses UV-B by tryptophan-mediated disruption of cross-dimer salt bridges. Science 335: 1492-1496
    • Cloix C, Kaiserli E, Heilmann M, Baxter KJ, Brown BA, O'Hara A, Smith BO, Christie JM, Jenkins GI (2012) C-terminal region of the UV-B photoreceptor UVR8 initiates signaling through interaction with the COP1 protein. Proc Natl Acad Sci USA 109: 16366-16370
    • Demkura PV, Abdala G, Baldwin IT, Ballaré CL (2010) Jasmonatedependent and -independent pathways mediate specific effects of solar ultraviolet B radiation on leaf phenolics and antiherbivore defense. Plant Physiol 152: 1084-1095
    • Demkura PV, Ballaré CL (2012) UVR8 mediates UV-B-induced Arabidopsis defense responses against Botrytis cinerea by controlling sinapate accumulation. Mol Plant 5: 642-652
    • Denslow SA, Rueschhoff EE, Daub ME (2007) Regulation of the Arabidopsis thaliana vitamin B6 biosynthesis genes by abiotic stress. Plant Physiol Biochem 45: 152-161
    • Favory JJ, Stec A, Gruber H, Rizzini L, Oravecz A, Funk M, Albert A, Cloix C, Jenkins GI, Oakeley EJ, et al (2009) Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. EMBO J 28: 591-601
    • Flint SD, Caldwell MM (2003) A biological spectral weighting function for ozone depletion research with higher plants. Physiol Plant 117: 137-144
    • Fuglevand G, Jackson JA, Jenkins GI (1996) UV-B, UV-A, and blue light signal transduction pathways interact synergistically to regulate chalcone synthase gene expression in Arabidopsis. Plant Cell 8: 2347-2357
    • González Besteiro MA, Bartels S, Albert A, Ulm R (2011) Arabidopsis MAP kinase phosphatase 1 and its target MAP kinases 3 and 6 antagonistically determine UV-B stress tolerance, independent of the UVR8 photoreceptor pathway. Plant J 68: 727-737
    • Götz M, Albert A, Stich S, Heller W, Scherb H, Krins A, Langebartels C, Seidlitz HK, Ernst D (2010) PAR modulation of the UV-dependent levels of flavonoid metabolites in Arabidopsis thaliana (L.) Heynh. leaf rosettes: cumulative effects after a whole vegetative growth period. Protoplasma 243: 95-103
    • Goulas Y, Cerovic ZG, Cartelat A, Moya I (2004) Dualex: a new instrument for field measurements of epidermal ultraviolet absorbance by chlorophyll fluorescence. Appl Opt 43: 4488-4496
    • Green AES, Sawada T, Shettle EP (1974) The middle ultraviolet reaching the ground. Photochem Photobiol 19: 251-259
    • Gruber H, Heijde M, Heller W, Albert A, Seidlitz HK, Ulm R (2010) Negative feedback regulation of UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. Proc Natl Acad Sci USA 107: 20132-20137
    • Heijde M, Ulm R (2012) UV-B photoreceptor-mediated signalling in plants. Trends Plant Sci 17: 230-237
    • Ibdah M, Krins A, Seidlitz HK, Heller W, Strack D, Vogt T (2002) Spectral dependence of flavonol and betacyanin accumulation in Mesembryanthemum crystallinum under enhanced ultraviolet radiation. Plant Cell Environ 25: 1145-1154
    • Izaguirre MM, Scopel AL, Baldwin IT, Ballaré CL (2003) Convergent responses to stress: solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora. Plant Physiol 132: 1755-1767
    • Jaspers P, Blomster T, Brosché M, Salojärvi J, Ahlfors R, Vainonen JP, Reddy RA, Immink R, Angenent G, Turck F, et al (2009) Unequally redundant RCD1 and SRO1 mediate stress and developmental responses and interact with transcription factors. Plant J 60: 268-279
    • Jenkins GI (2009) Signal transduction in responses to UV-B radiation. Annu Rev Plant Biol 60: 407-431
    • Kalbina I, Li S, Kalbin G, Björn LO, Strid Å (2008) Two separate UV-B radiation wavelength regions control expression of different molecular markers in Arabidopsis thaliana. Funct Plant Biol 35: 222-227
    • Kleine T, Kindgren P, Benedict C, Hendrickson L, Strand Å (2007) Genome-wide gene expression analysis reveals a critical role for CRYPTOCHROME1 in the response of Arabidopsis to high irradiance. Plant Physiol 144: 1391-1406
    • Kliebenstein DJ, Lim JE, Landry LG, Last RL (2002) Arabidopsis UVR8 regulates ultraviolet-B signal transduction and tolerance and contains sequence similarity to human regulator of chromatin condensation 1. Plant Physiol 130: 234-243
    • Kotilainen T, Venäläinen T, Tegelberg R, Lindfors A, Julkunen-Tiitto R, Sutinen S, O'Hara RB, Aphalo PJ (2009) Assessment of UV biological spectral weighting functions for phenolic metabolites and growth responses in silver birch seedlings. Photochem Photobiol 85: 1346-1355
    • Kreuter A, Blumthaler M (2009) Stray light correction for solar measurements using array spectrometers. Rev Sci Instrum 80: 096108
    • Lindfors A, Heikkilä A, Kaurola J, Koskela T, Lakkala K (2009) Reconstruction of solar spectral surface UV irradiances using radiative transfer simulations. Photochem Photobiol 85: 1233-1239
    • Mackerness SAH, Surplus SL, Blake P, John CF, Buchanan-Wollaston V, Jordan BR, Thomas B (1999) Ultraviolet-B-induced stress and changes in gene expression in Arabidopsis thaliana: role of signalling pathways controlled by jasmonic acid, ethylene and reactive oxygen species. Plant Cell Environ 22: 1413-1423
    • Malitsky S, Blum E, Less H, Venger I, Elbaz M, Morin S, Eshed Y, Aharoni A (2008) The transcript and metabolite networks affected by the two clades of Arabidopsis glucosinolate biosynthesis regulators. Plant Physiol 148: 2021-2049
    • Morales LO, Tegelberg R, Brosché M, Keinänen M, Lindfors A, Aphalo PJ (2010) Effects of solar UV-A and UV-B radiation on gene expression and phenolic accumulation in Betula pendula leaves. Tree Physiol 30: 923-934
    • Onda Y, Yagi Y, Saito Y, Takenaka N, Toyoshima Y (2008) Light induction of Arabidopsis SIG1 and SIG5 transcripts in mature leaves: differential roles of cryptochrome 1 and cryptochrome 2 and dual function of SIG5 in the recognition of plastid promoters. Plant J 55: 968-978
    • Oravecz A, Baumann A, Máté Z, Brzezinska A, Molinier J, Oakeley EJ, Adám E, Schäfer E, Nagy F, Ulm R (2006) CONSTITUTIVELY PHOTOMORPHOGENIC1 is required for the UV-B response in Arabidopsis. Plant Cell 18: 1975-1990
    • Pinheiro JC, Bates DM (2000) Mixed-Effects Models in S and S-Plus. Springer, New York
    • R Development Core Team (2010) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna. http://www.R-project.org (October 15, 2010)
    • Ristilä M, Strid H, Eriksson LA, Strid A, Sävenstrand H (2011) The role of the pyridoxine (vitamin B6) biosynthesis enzyme PDX1 in ultraviolet-B radiation responses in plants. Plant Physiol Biochem 49: 284-292
    • Rizzini L, Favory JJ, Cloix C, Faggionato D, O'Hara A, Kaiserli E, Baumeister R, Schäfer E, Nagy F, Jenkins GI, et al (2011) Perception of UV-B by the Arabidopsis UVR8 protein. Science 332: 103-106
    • Sarkar D (2008) Lattice: Multivariate Data Visualization with R. Springer, New York
    • Thimijan RW, Carns HR, Campbell LE (1978) Radiation Sources and Related Environmental Control for Biological and Climatic Effects UV Research (BACER). Final report (EPA-IAG-DG-0168). Environmental Protection Agency, Washington, DC
    • Ulm R, Baumann A, Oravecz A, Máté Z, Adám E, Oakeley EJ, Schäfer E, Nagy F (2004) Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis. Proc Natl Acad Sci USA 101: 1397-1402
    • Wade HK, Bibikova TN, Valentine WJ, Jenkins GI (2001) Interactions within a network of phytochrome, cryptochrome and UV-B phototransduction pathways regulate chalcone synthase gene expression in Arabidopsis leaf tissue. Plant J 25: 675-685
    • Wargent JJ, Gegas VC, Jenkins GI, Doonan JH, Paul ND (2009a) UVR8 in Arabidopsis thaliana regulates multiple aspects of cellular differentiation during leaf development in response to ultraviolet B radiation. New Phytol 183: 315-326
    • Wargent JJ, Moore JP, Roland Ennos A, Paul ND (2009b) Ultraviolet radiation as a limiting factor in leaf expansion and development. Photochem Photobiol 85: 279-286
    • Warnes GR (2005) gregmisc: Greg's Miscellaneous Functions. R package version 2.1.1. http://cran.r-project.org/package=gregmisc (October 15, 2010)
    • Wettenhall JM, Smyth GK (2004) limmaGUI: a graphical user interface for linear modeling of microarray data. Bioinformatics 20: 3705-3706
    • Wrzaczek M, Brosché M, Salojärvi J, Kangasjärvi S, Idänheimo N, Mersmann S, Robatzek S, Karpinski S, Karpinska B, Kangasjärvi J (2010) Transcriptional regulation of the CRK/DUF26 group of receptorlike protein kinases by ozone and plant hormones in Arabidopsis. BMC Plant Biol 10: 95
    • Wu D, Hu Q, Yan Z, Chen W, Yan C, Huang X, Zhang J, Yang P, Deng H, Wang J, et al (2012) Structural basis of ultraviolet-B perception by UVR8. Nature 484: 214-219
    • Wu M, Grahn E, Eriksson LA, Strid Å (2011) Computational evidence for the role of Arabidopsis thaliana UVR8 as UV-B photoreceptor and identification of its chromophore amino acids. J Chem Inf Model 51: 1287-1295
    • Ylianttila L, Visuri R, Huurto L, Jokela K (2005) Evaluation of a singlemonochromator diode array spectroradiometer for sunbed UV-radiation measurements. Photochem Photobiol 81: 333-341
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